Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit as antiaging

Utami, Sri; Sachrowardi, Qomariyah; Damayanti, Ndaru Andri; Wardhana, Arroyan; Syarif, Irfan; Nafik, Said; Arrahman, Betharie Cendera; Kusuma, Hanna Sari Widya; Widowati, Wahyu

doi:10.15171/jhp.2018.15

Cited by 23 publications

(17 citation statements)

References 24 publications

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“…The higher ability of the sample to inhibit elastase activity, the less Sucala3 is formed (yellow was slightly/clear). 12,13,14,20 IC50 values of black soybean extract and daidzein were 93.36 ± 6.39 μg/ml and 57.35±5.64 μg/ml. Budaraga et al reported strong activity around 50-100 µg/ml, the stronger the activity with the smaller the value.…”

Section: Discussionmentioning

confidence: 96%

Antioxidant and Elastase Inhibitor from Black Soybean (Glycine max L.) and Its Compound (Daidzein)

Juliana

Lister

Girsang

et al. 2020

J. Biomed. Transl. Res.

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Background: Skin aging occurs along with age. Genetic, nutrients, hormones, and sun exposure can affect directly and indirectly the structure of the skin. These things will induce an increase in free radicals which disrupts one of the most important enzymes in the extracellular matrix which is elastase enzyme. High enzyme synthesis will cause skin wrinkle. Free radicals can be inhibited by the presence of antioxidants. The plant contains natural phytochemical compounds which act as antioxidants and anti aging. Black soybeans are rich in daidzein compound, which protect against free radicals and prevent premature aging.Objective: This research evaluates the antioxidant and antiaging potential of Black Soybean (Glycine max L.) extract and its compound, daidzein.Methods: Analysis of antioxidants from black soybean extract and daidzein were carried out using ABTS scavenging activity assay. The antiaging assay was carried out through inhibition of elastase enzyme.Results: Black soybean extract had lower IC50 value of ABTS scavenging activity around 77.39±4.05 μg/ml better than daidzein with IC50 of 83.34±3.89 μg/ml. The results of elastase inhibition activity assay showed that daidzein compound has a lower IC50 value, 57.35±5.64 μg/ml compared to black soybean extract with IC50 value, 93.36±6.39 μg/ml.Conclusion: Black soybean extract had higher antioxidant. Daidzein had better elastase inhibition activity compared to black soybean extract.

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Section: Discussionmentioning

confidence: 96%

Antioxidant and Elastase Inhibitor from Black Soybean (Glycine max L.) and Its Compound (Daidzein)

Juliana

Lister

Girsang

et al. 2020

J. Biomed. Transl. Res.

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“…The plates were incubated for 15 minutes at 25 °C using a rotator. Absorbance was measured using a microplate reader at a wavelength of 410 nm (Widowati et al, 2017;Girsang et al, 2020;Utami et al, 2018;Widowati et al, 2018b;Juliana et al, 2020) Ac: control absorbance As: sample absorbance…”

Section: Anti-elastase Inhibition Activity Testmentioning

confidence: 99%

“…Some examples of plants that have antioxidant and antiaging effects are roselle roses (Hisbiscus sabdarifa) (Widowati et al, 2017), jasmine (Jasminum sambac) (Widowati et al, 2018b), ripe sesoot fruit (Garcinia picrorrhiza Miq.) (Utami et al, 2018), black soybeans (Glycine max [L.] Merr) (Asan et al, 2019), dragon fruit peel (Hylocereus polyrhizus) (Liana et al, 2019), pineapple (Vrianty et al, 2019), and zalacca skin (Salacca zalacca (Gaert.) Voss) (Girsang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Elastase Inhibitor Potential of Petals and Receptacle of Rose Flower (Rosa damascena)

Mawarni¹,

Ginting²,

Chiuman³

et al. 2020

psr

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Free radicals can cause damage to cells or tissues, autoimmune diseases, degenerative diseases, or cancer. Therefore, the body needs important substances, namely antioxidants that can help protect the body by reducing negative effect from free radicals. Rose flower (Rosa damascena) has anthocyanin pigment which belongs to flavonoid group which has a function as antioxidant or free radical scavenger. This study aims to determine antioxidant and anti-elastase potentials of rose petals and receptacles. The method used in this study was a qualitative phytochemical test to determine the compounds contained in the Rose Petal Extract (RPE) and Rose Receptacle Extract (RRE), ABTS ((2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid))-reducing assay to determine antioxidant activity, and antiaging test with anti-elastase assay. RPE and RRE contained flavonoids, phenols, tannins and alkaloids, but did not contain saponins. RPE contained triterpenoids and terpenoids, while RRE contained steroids, but did not contain terpenoids. The inhibitory concentration (IC 50) values in the ABTS reducing assay were 4.46 ± 0.34 μg/mL (RPE) and 15.49 ± 0.23 μg/mL (RRE), while the results of the anti-elastase assay were 17.51 ± 1.47 μg/ mL (RPE) and 58.91 ± 2.31 μg/mL (RRE). Both RPE and RRE are potent antioxidant and antielastase, and RPE is more active than RRE in these assays.

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“…Collagenase inhibitory activity assay was conducted based on the method that was elaborated by Wittenauer et al (2015) with some modification (Utami et al, 2018;Widowati et al, 2018Widowati et al, , 2016Wittenauer, 2015). The mixture solution consisting of 30 µL samples with serial concentration (250, 125, 62.5, 31.25, 15.3, and 7.81 µg/ mL), 10 µL collagenase from clostridium histolyticum enzyme ((0.1 mg/mL, sigma C8051), and 60 µL buffer tricine (50 mM tricine, 10 mM calcium chloride, 400 mM Sodium chloride, pH 7.5) were incubated at 37 o C for 20 min.…”

Section: Collagenase Inhibitory Activity Assaymentioning

confidence: 99%

Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin

Geeta

Widodo²,

Widowati

et al. 2019

psr

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Background: Skin aging is a complex natural process caused by both intrinsic or genetically programmed aging and extrinsic aging caused by environmental factors, such as free radical. The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural compounds from plants are one of the sources of antioxidant and anti-collagenase that has ability to prevent aging. Genistein and Epicatechin are the major phenolic compound found in G. max. Objectives: This research was to evaluate the antiaging potential of genistein and epicatechin through antioxidant activity assay (ABTS-reducing activity assay) and collagenase inhibitory activity assay. Methods: Antioxidant analysis of Genistein and Epicatechin was performed by 2,2'-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) reducing activity Assay. Antiaging assay was conducted through inhibitory of collagenase, one of important enzyme in aging process. Results: ABTS-reducing activity assay showed that both compounds had great ABTS-reducing activity in which epicatechin had better activity than genistein. Epicatechin had low value of IC 50 ABTS-reducing activity around 14.39 µg/ml better than genistein with IC 50 about 43.17 µg/ml. In terms of collagenase inhibitory activity assay, epicatechin had lower value of IC 50 (9.08+-3.46 ug/ml), better than genistein (98.74+-4.25 ug/ml). Conclusion: Epicatechin had higher antioxidant and anti-collagenase activity compared to Genistein.

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Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit as antiaging

Abstract: Extract of G. picrorrhiza has potential to increase antioxidant, anticollagenase, and antielastase activities. Therefore, more investigations need for identification of the active compounds in G. picrorrhiza extract involved in antioxidant and antiaging activities.

Cited by 23 publications

References 24 publications

Antioxidant and Elastase Inhibitor from Black Soybean (Glycine max L.) and Its Compound (Daidzein)

Antioxidant and Elastase Inhibitor from Black Soybean (Glycine max L.) and Its Compound (Daidzein)

Antioxidant and Elastase Inhibitor Potential of Petals and Receptacle of Rose Flower (Rosa damascena)

Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin

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