Antipoliovirus Flavonoids from Psiadia Dentata

Abstract

The search for antiviral agents against vesicular stomatitis virus, herpes simplex virus type 1 and poliovirus type 2 in plants extracts, led to the isolation of two antipoliovirus flavonoids from the medicinal plant Psiadia dentata (Cass.) DC, Asteraceae: 3-methylkaempferol and 3,4′-dimethylkaempferol. The antipoliovirus activity of both compounds was estimated by comparison with 3-methylquercetin, guanidine and Ro-090179. The most potent inhibitor of poliovirus replication was 3-methylkaempferol, and therefore we investigated its mechanism of action. We showed, using the inhibition of [ 3 H]uridine incorporation in viral RNA and performing a dot-blot with one RNA probe specific for the poliovirus genomic strand RNA, that 3-methylkaempferol inhibits the genomic RNA synthesis of poliovirus.

Antiplasmodial, anti-inflammatory and cytotoxic activities of various plant extracts from the Mascarene Archipelago

“…Methoxyflavones and coumarins have been isolated from Psiadia dentata (Fortin et al, 2001;Jakobsen et al, 2001). Robin et al (2001) revealed that 3-methylkaempferol inhibited the genomic RNA synthesis of poliovirus. Aside from this aspect, Psiadia dentata needs to be investigated further.…”

Selective inhibitors of picornavirus replication

“…231,233 When added at the time of infection, however, both plus-and minus-strand viral RNA synthesis were blocked, but this might be due to the fact that there is not enough genomic RNA to serve as a template to give rise to significant amounts of negative-stranded RNA, detectable by hybridization. 234 Other flavonoids isolated from medicinal plants include two flavonoids from Psiadia dentata (3-methylkaempferol and 3,4 0 -dimethylkaempferol), which are shown to inhibit the synthesis of poliovirus (þ)RNA, 235 and a flavonoid from Pterocaulon sphacelatum, also exhibiting protection against poliovirus-induced CPE formation. 236 Several synthetic flavonoid and isoflavonoid derivatives were evaluated for their potential antipicornavirus activity.…”

Genistein inhibits the replication of avian leucosis virus subgroup J in DF-1 cells

“…Genistein can inhibit the phosphorylations and activations of ATF-2, NF-B and other transcription factors (Evers et al, 2005;Vela et al, 2008). Genistein can also inhibit the phosphorylation of viral proteins and polypeptides (Yura et al, 1993), inhibit viral gene expression and protein function (Kim et al, 2006;Robin et al, 2001), inhibit the tyrosine phosphorylation of viral glycoproteins, (Akula et al, 2002) etc.…”