Antiproliferative Activity of Marrubium persicum Extract in the MCF-7 Human Breast Cancer Cell Line

Abstract

Aim: Developing antitumor drugs from natural products is receiving increasing interest worldwide due to limitations and side effects of therapy strategies for the second leading cause of disease related mortality, cancer. Methods: The antiproliferative activity of a methanolic extract from the aerial parts of Marrubium persicum extract was assessed with the MCF-7 breast cancer cell line using the MTT test for cell viability and cytotoxicity indices. In addition, antioxidant properties of the extract were evaluated by measuring its ability to scavenge free DPPH radicals. Moreover, the total phenolic and flavonoid content of the extract was determined based on Folin-Ciocalteu and colorimetric aluminum chloride methods. Results: The findings of the study for the antiproliferative activity of the methanolic extract of M. persicum showed that growth of MCF-7 cells was inhibited by the extract in a dose and time dependent manner, where a gradual increase of cytotoxicity effect has been achieved setting out on 200 µg/mL concentration of the plant extract. The antioxidant assay revealed that the extract was a strong scavenger of DPPH radicals with an RC 50 value of 52 µg/mL. The total phenolic and flavonoids content of the plant extract was 409.3 mg gallic acid equivalent and 168.9 mg quercetin equivalent per 100g of dry plant material. Conclusion: Overall, M. persicum possesses potential antiproliferative and antioxidant activities on the malignant MCF-7 cell line that could be attributed to the high content of phenolics and flavonoids, and therefore warrants further exploration.

Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

“…(Uddin et al, 2009). Several recent studies have investigated the cytotoxicity of natural plant extracts against human cervical and breast cancer cell lines (MCF-7 and HeLa) (Hamedeyazdan et al, 2012;Zhao et al, 2014). Proliferation and viability of MCF-7 and HeLa cells were decreased after treatment with DOI:http://dx.doi.org/10.7314/APJCP.2014.15.17.7229 Cytotoxicity of Trichoderma spp.…”

“…Culture Filtrates against Human Cervical and Breast Cancer Cell Lines different plant extracts. This decrease in cell proliferation and cell viability can be related not only to the effection of the arresting cell cycle, but also to the induction of apoptosis (Hamedeyazdan et al, 2012). Cytotoxic activities of Trichoderma spp.…”

Doxorubicin Changes Bax /Bcl-xL Ratio, Caspase-8 and 9 in Breast Cancer Cells

“…[30][31][32][33] MCF-7 cell line is an excellent in vitro model for studying the mechanisms of chemoresistance as it is susceptible to apoptosis and applied for various investigations on apoptosis or survival of cancer cells. [34][35][36] Although the extensive clinical application of doxorubicin for cancer patients has been studied, its anti-proliferative and death-inducing signalling cascades are yet unclear. In this study, we investigated the molecular basis of induction of apoptosis by doxorubicin on MCF-7 cells.…”

An investigation on cardioprotective potential of Marrubium vulgare aqueous fraction against ischaemia-reperfusion injury in isolated rat heart

“…This perennial plant is widely distributed in northern and eastern provinces of Iran [10,27]. M. vulgare is a popular medicinal herb and in traditional medicine has been used as a neurosedative, hypotensive, antispasmodic, antinociceptive, antidiabetic and anti-inflammatory agent.…”