Antitumor Activity for Gold (III) Complex by High Content Screening Technique (HCS) and Cell Viability Assay

Abstract

Multipara metric analysis of compound toxicity at the level of individual cells using flow cytometry and cellular imaging-based approaches such as High Content Screening (HCS) have played key roles in the detection of toxicity and classification of compounds based on observed patterns of reversible and irreversible cellular injury. Gold III complex (AuL 2 ) of bidentate ligand derived from the cyclization reaction of Schiff base of 4-amino-5-phenyl-4h-1,2,4-triazole-3-thiol with thioglycolic acid was synthesized and characterized by using melting point, FTIR spectroscopy, 1 HNMR, UV-Visible spectroscopy and elemental analysis. The presence of chloride counter ion in complex was supported by conductivity measurement and the presence of hydrated water was supported by thermal gravimetric studies. We examine the cytotoxic effects of gold complex and its ligand in one cultured cellular models (MCF7 cell line) by High Content Screening (HCS) and analysis and cell viability assay (MMT assay). The inhibitory effect of AuL 2 on breast cancer cell growth was due to induction of apoptosis as evidenced by annexin V staining and cell shrinkage. The study found that AuL 2 -mediated lead to disruption of Mitochondrial Membrane Potential (MMP), cell membrane permeability, nuclear condensation, fragmentation and release of cytochrome c from the mitochondria into the cytosol and also suggesting AuL 2 as a potential MCF7 inhibitor compared to doxorubicin as positive control. In this study, data showed that gold (III) complex AuL 2 may have therapeutic value in breast cancer treatment worthy of further development.

Characterization and acid-catalysed depolymerization of condensed tannins derived from larch bark

“…The weak bands at 699 cm À1 and 668 cm À1 were attributed to C-S bond. 45,46 These bands were not observed in the spectrum of LT but were present in the spectra of DLT-BM and DLT-ML. The presence of these bands conrmed that the mercapto group was added to the avonoid structure of larch tannin, indicating that the larch tannin was successfully depolymerized and the thiol-nucleophiles had a positive effect on the depolymerization of larch tannin.…”

Effect of Anti Diabetic Drugs on Lipid Profile in Patients with Type 2 Diabetes Mellitus

“…Insulin resistance occurs when the cells become less sensitive to the effects of insulin [7]. In continuation of previous studies [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22], herein we are reporting study lipid profile level were quantitatively determining by enzymatic methods, in addition to that fasting plasma glucose (FPG), Glycated hemoglobin (HbA1c%) and body mass index (BMI) were identified.…”

Cytotoxicity anticancer activities of anastrozole against breast, liver hepatocellular, and prostate cancer cells

“…The high-content screening (HCS) assay was performed at the Centre for Natural Product Research and Drug Discovery, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia between November 2015 to February 2016. The MMT and HCS assays were carried out based on standard procedures 22,23…”