Antiviral activity of agarwood Aquilaria malaccensis lamk and Gyrinops versteegii (Gilg.) Domke leaves ethanolic extract against dengue serotype 3 virus in vitro

Masita, Rahmi; Nuringtyas, Tri Rini; Wijayanti, Nastiti; Hidayati, Lisna

doi:10.1063/5.0002645

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…CITES (The Convention on International Trade in Endangered Species of Wild Flora dan Fauna) juga mengelompokan status konservasi G. versteegii ke dalam daftar appendix II. Padahal penelitian mengenai G. versteegii semakin banyak dilakukan dan didukung oleh kemampuan bioaktivitasnya sebagai antioksidan (Nuringtyas et al, 2018), antikanker (Nuringtyas et al, 2018;Wardana et al, 2019) dan antivirus (Masita et al, 2020).…”

Section: Pendahuluanunclassified

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Marisa

Hidayati

Sasongko

et al. 2021

JBT

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Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This plant has been threatened by overexploitation which leads on decreasing in the natural population. This research aimed to induce in vitro callus formation to support the sustainable utilization of G. versteegii, which in the end, may support plant productivity. Callus induction was investigated using cotyledon and cultured on MS medium supplemented with several combination of plant growth regulators. The observations were done for the number of explants turned into callus and the duration for the first callus formation. The results showed that combination of 3 mg/L NAA + 0.5 mg/L BAP was recorded as the best combination for callus induction (63.63%). Callus with friable structure and bright color are obtained within nine days of incubation and showed the characteristic of embryonic callus. This result is expected to give a significant opportunity to conserve the natural population of G. versteegii.

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Section: Pendahuluanunclassified

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Marisa

Hidayati

Sasongko

et al. 2021

JBT

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“…Aquilaria malaccensis is a potential pharmacological plant due to its capacity to generate various secondary metabolites. Aquilaria malaccensis leaves extract has antioxidant (Hendra et al, 2016;Halim et al, 2022), antiviral against dengue virus (Masita et al, 2020) and immunomodulatory potential (Batubara et al, 2021;Wahid et al, 2022;Yana et al, 2022). Furthermore, the stem has been referenced for anticancer activities (Hegde et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Isolation and morphological identification of endophytic fungi from leaves and stems of Aquilaria malaccensis Lamk. originating from Java Island, Indonesia

A.¹,

Nuringtyas²

2023

MJM

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Aims: Endophytic fungi are the remarkable category of host-associated fungal community that invades the intercellular regions of host tissues, benefiting their host while obtaining an advantage. Fungal endophytes have lately attracted prominence as a source of active secondary metabolites. This investigation aimed to identify fungal endophytes that reside inside the leaves and stems of Aquilaria malaccensis. Methodology and results: Healthy A. malaccensis stems and leaves samples were collected. Clean leaves and stems were cut to a size of 1 cm, followed by sterilization using 75% ethanol for 1 min, 3% sodium hypochlorite solution for 1 min, and finally, rinsing with sterile water 3 times for 1 min and drying with sterile paper. The sterile samples were put onto Potato Dextrose Agar (PDA) media containing chloramphenicol for 7-14 days until the mycelium grew for morphological identification under a light microscope. Five endophytic fungi were recovered from leaves, while nine endophytic fungi were obtained from stems. Using morphological approaches, nine of the endophytes had observed to produce conidia fungi, whereas the others did not. Neopestalotiopsis sp., Aspergillus sp., Arthrinium sp., Curvularia sp., Podospora sp., Mucor sp. and Verticillium sp. were identified as nine of the fourteen endophytes. Conclusion, significance and impact of study:The number of endophytic fungi discovered in different organs varies. Not all endophytic fungi that grow can create sexual phages. Six genera of endophytic fungi were identified.

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“…Agarwood plants are naturally distributed in some countries such as Bangladesh, India, Indonesia, Malaysia, Myanmar, and Vietnam. (9) Agarwood leaves have been studied for their application potential as an antioxidant, antibacterial (10), antiviral (11), and antihyperglycemic (12). The leaf extracts contained secondary metabolites with anticancer activity.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Activity of Agarwood Aquilaria malaccensis Lamk. Leaf Extracts on Staphylococcus aureus-infected Macrophages in vitro

et al. 2022

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BACKGROUND: Aquilaria malaccensis has been consumed as herbal medicine, and in vitro study showed that the leaf extract possesses high antioxidant activities. A brief preliminary study indicated that A. malaccensis showed a promising immunomodulatory activity when evaluated using latex beads. This current study aimed to evaluate the immunomodulatory activity of A. malaccensis leaf extract on the macrophage, which was challenged with pathogenic bacteria Staphylococcus aureus.METHODS: Bioactivity was determined by evaluating the phagocytic capacity of macrophages isolated from Mus musculus against S. aureus. First, the cytotoxicity of extracts on macrophages was evaluated using MTT assays, and the IC50 value was used to determine the dose of immunomodulatory assays. The highest toxicity was observed on chloroform extract with an IC50 value of 111.4 µg/mL. Therefore, the treatment was 100 and 50 µg/mL. Two parameters, including the phagocytic activity and phagocytic capacity of macrophages infected with S. aureus, were used to evaluate immunomodulatory activity. The analysis of variance was done at p<0.05 to determine the significant difference among treatments.RESULTS: Chloroform and ethanol extracts at a 50 µg/mL concentration showed the best results with the phagocytic activity of 82.33%±9.61% and 80.33±1.53%. The ethyl acetate showed lower phagocytic activities of 70.67±1.53. All extracts significantly increased phagocytic activity and phagocytic capacity, and the results differed significantly between negative and positive controls. Thin-layer chromatography indicated that the extract contained terpenoid, flavonoid, phenolic, and tannin.CONCLUSION: A. malaccensis leaf extracts showed immunomodulatory activity. Both chloroform and ethanol extracts showed comparable activity, while the ethyl acetate extract was lower. The extracts contained diverse bioactive compounds that may support activating macrophage cells for immunomodulatory activity.KEYWORDS: Aquilaria malaccensis, immunomodulator, phagocytosis, macrophages, Staphylococcus aureus

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Antiviral activity of agarwood Aquilaria malaccensis lamk and Gyrinops versteegii (Gilg.) Domke leaves ethanolic extract against dengue serotype 3 virus in vitro

Cited by 3 publications

References 18 publications

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Isolation and morphological identification of endophytic fungi from leaves and stems of Aquilaria malaccensis Lamk. originating from Java Island, Indonesia

Immunomodulatory Activity of Agarwood Aquilaria malaccensis Lamk. Leaf Extracts on Staphylococcus aureus-infected Macrophages in vitro

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