Antiwrinkle and antimelanogenesis activity of the ethanol extracts of <i>Lespedeza cuneata</i> G. Don for development of the cosmeceutical ingredients

Lee, Jongsung; Ji, Junfu; Park, See‐Hyoung

doi:10.1002/fsn3.682

Cited by 5 publications

(10 citation statements)

References 53 publications

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“…In addition, extracts of L. cuneata were reported to have hepatoprotective and antidiabetic effects [4], anticancer and antioxidative activity, and inhibitory effects on cell proliferation [5]. The useful cosmetic effects of L. cuneata extracts, such as antiwrinkle, antimelanogenic, anti-aging, and anti-oxidative effects, have also been reported [6,7]. L. cuneata also showed pharmacological effects against male and female diseases, such as protective effects against testicular and ovarian diseases [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…In the current study, we found in a screening test that the MeOH extract of L. cuneata exerted inhibitory effect on metabolic activity in MCF-7 human breast cancer cells, which led us to perform further phytochemical analysis and identify the bioactive compound(s) responsible for these effects. Bioassay-guided fractionation and chemical investigation of the MeOH extract led to the isolation of nine compounds (four lignan glycosides (1)(2)(3)(4), three flavonoid glycosides (5)(6)(7), and two phenolic compounds (8 and 9)). The structures of the isolated compounds were identified by LC-MS analysis and their spectroscopic data and physical data were compared with previously reported values.…”

Section: Introductionmentioning

confidence: 99%

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Aviculin Isolated from Lespedeza cuneata Induce Apoptosis in Breast Cancer Cells through Mitochondria-Mediated Caspase Activation Pathway

Lee

et al. 2020

Molecules

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The global incidence of breast cancer has increased. However, there are many impediments to the development of safe and effective anticancer drugs. The aim of the present study was to evaluate the effect of aviculin isolated from Lespedeza cuneata (Dum. Cours.) G. Don. (Fabaceae) on MCF-7 human breast cancer cells and determine the underlying mechanism. Using the bioassay-guided isolation by water soluble tetrazolium salt (WST-1)-based Ez-Cytox assay, nine compounds (four lignan glycosides (1–4), three flavonoid glycosides (5–7), and two phenolic compounds (8 and 9)) were isolated from the ethyl acetate (EA) fraction of the L. cuneata methanolic extract. Of these, aviculin (2), a lignan glycoside, was the only compound that reduced metabolic activity on MCF-7 cells below 50% (IC50: 75.47 ± 2.23 μM). The underlying mechanism was analyzed using the annexin V Alexa Fluor 488 binding assay and Western blotting. Aviculin (2) was found to induce apoptotic cell death through the intrinsic apoptosis pathway, as indicated by the increased expression of initiator caspase-9, executioner caspase-7, and poly (ADP-ribose) polymerase (PARP). Aviculin (2)-induced apoptotic cell death was accompanied by an increase in the Bax/Bcl-2 ratio. These findings demonstrated that aviculin (2) could induce breast cancer cell apoptosis through the intrinsic apoptosis pathway, and it can therefore be considered an excellent candidate for herbal treatment of breast cancer.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aviculin Isolated from Lespedeza cuneata Induce Apoptosis in Breast Cancer Cells through Mitochondria-Mediated Caspase Activation Pathway

Lee

et al. 2020

Molecules

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“…Over-produced melanin should be controlled to maintain the skin integrity. That is why the discovery of novel tyrosinase inhibitors for the development of cosmeceutical formulations has received a significant interest [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

“…There are numerous studies on bioactive materials isolated from plants that are currently used in cosmeceutical formulations, but very few studies on bioactive materials from bacteria are available [9,21,[24][25][26][27]31]. This study aimed to evaluate the cosmeceutical potential of ethyl acetate extracts from the bacterial strain Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Development of Multifunctional Cosmetic Cream Using Bioactive Materials from Streptomyces sp. T65 with Synthesized Mesoporous Silica Particles SBA-15

Dahal

Nguyen

Shim³

et al. 2020

Antioxidants

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Various cosmetics having a single function are increasingly being used, but cosmetics having multifunctional activities remain limited. We aimed to develop a multifunctional cosmetic cream having antioxidant, anti-tyrosinase, anti-aging and antimicrobial activities. Antimicrobial activities were performed by disc-diffusion method. Cell toxicity and cell proliferations were evaluated in a 96-well plate with different cell lines such as HaCaT, RAW264.7, CCD-986Sk, B16F1, and B16F10. Mushroom tyrosinase inhibition, elastase inhibition, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were evaluated and IC50 was calculated. Mesoporous silica particle was synthesized using Pluronic P123 and tetraethyl ortho-silicate (TEOS). Facial pictures were captured by VISIA-CR (Facial Imaging System for Clinical Research). Roughness of image was analysed by PRIMOS software and brightness of image was analyzed by Chromameter CR-400. The crude product of strain T65 inhibited the different human pathogenic bacteria such as Bacillus subtilis, Escherichia coli, Propionibacterium acnes, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The IC50 of T65 crude product for mushroom tyrosinase, elastase, and DPPH radical scavenging activities were 58.73, 14.68, and 6.31 µg/mL, respectively. T65 crude product proliferated collagen type I in CCD-986Sk cell up to 145.91% ± 9.11% (mean ± SD; mean of 24, 48, and 72 h) at 250 pg/mL. Synthesized mesoporous particles (SBA-15) confirmed the sustainable performance by control-release for three days. Formulated functional cosmetic cream containing T65 embedded SBA-15, significantly decreased the skin roughness by 4.670% and increased the skin brightness by 0.472% after application of 4 weeks. T65 crude product inhibited both Gram-positive and Gram-negative pathogens. Synthesized mesoporous particle, SBA-15, confirmed the physiologically active substance was released in sustainable release condition. T65 crude product showed impeccable antimicrobial, antioxidant, anti-aging, and whitening activities with non-cytotoxic effects to different cell lines related to the human skin.

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“…New tyrosinase inhibitors are continually required to deal with melanogenesis caused problems effectively with a few side effects. Therefore, the effects of various natural source extracts on human skin whitening and anti-melanogenesis have drawn significant attention, and numerous studies have been conducted to verify their benefits in cosmetic formulations [8,9].…”

Section: Introductionmentioning

confidence: 99%

Inonotus Obliquus extracts as an inhibitor of α-MSH-induced melanogenesis in B16F10 mouse melanoma cells

Lee¹,

Jun²

2018

Preprint

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Melanogenesis is a biosynthetic pathway for producing of the pigment melanin in human skin. Tyrosinase, a key enzyme, catalyzing is the first step in melanogenesis and the downregulation of the tyrosinase enzyme activity is the most reported method for anti-melanogenesis. According to the hyperpigmentation as an important issue in cosmetic industry, there is a big demand for melanogenesis inhibitors. In the present study, we identified the anti-melanogenic effect of Inonotus Obliquus in α-MSH-induced B16F10 mouse melanoma cells as a new inhibitor. Comparing with control and Inonotus Obliquus extracts treated B16F10, we identified melanin contents, tyrosinase activity, tyrosinase mRNA and protein expression, MITF activity using a constructed plasmid. As shown in these results, we demonstrated that Inonotus Obliquus extracts down-regulated melanin synthesis using down-regulating activity and expression of tyrosinase which is key enzyme to produce melanin. In addition, we revealed expression of tyrosinase is regulated by MITF through repressing MITF transcriptional activity. Inonotus Obliquus extracts has potential to repress melanogenesis and decreased of hyperpigmentation and to use as cosmetic ingredient.

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Antiwrinkle and antimelanogenesis activity of the ethanol extracts of Lespedeza cuneata G. Don for development of the cosmeceutical ingredients

Cited by 5 publications

References 53 publications

Aviculin Isolated from Lespedeza cuneata Induce Apoptosis in Breast Cancer Cells through Mitochondria-Mediated Caspase Activation Pathway

Aviculin Isolated from Lespedeza cuneata Induce Apoptosis in Breast Cancer Cells through Mitochondria-Mediated Caspase Activation Pathway

Development of Multifunctional Cosmetic Cream Using Bioactive Materials from Streptomyces sp. T65 with Synthesized Mesoporous Silica Particles SBA-15

Inonotus Obliquus extracts as an inhibitor of α-MSH-induced melanogenesis in B16F10 mouse melanoma cells

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