Application of phase optimized liquid chromatography to oligopeptide separations

Liquid Chromatography with mass spectrometry analysis of mycotoxins in food samples using silica hydride based stationary phases

“…However, phenyl HPLC separation materials, while predominantly used in the reversed‐phase mode, could offer different selectivity due to the possibility of π–π interactions with the analytes . For example, phenyl phases have been used for analyses of polycyclic aromatic hydrocarbons , nutraceuticals , oligopeptides , guaifenesin , methyl‐ and propyl‐parabens , flavonoids , and camptothecin and related compounds . However, it appears that there is only one study related to the use of phenyl columns for the analysis of toxins in cereal grains .…”

Rapid determination of X-ray contrast agent iomeprol in human plasma by on-line solid-phase extraction coupled with phase optimized liquid chromatography

“…However, using the gradient elution technique, signal instability and baseline drift may increase, and an additional re-equilibration time is required [20,21]. Very recently, phase optimized liquid chromatography (POPLC) has been introduced as a new way of selectivity tuning in method optimization [22,23]. The POPLC approach follows a strategy which is based on the optimized combination of column segments with different functionalities.…”

“…In particular, the reliability and performance of several relevant detection techniques (especially mass spectrometry, refractive index detection and electrochemical detection) have been considerably improved [20]. In addition to the use of isocratic elution, the advantages and benefits of POPLC include optimized analysis time by software, high sample throughput, recycling of the mobile phase, adaptable column length, no guard column, fast method development and optimization of existing methods [22,25].…”

Gradient stationary phase optimized selectivity liquid chromatography with conventional columns

“…Many applications of POPLC with isocratic analysis were reported. [7][8][9][10][11][12] In our previous studies, the isocratic SOSLC protocol was rst applied in multiple step isocratic analysis of 27 steroids. 13 Successful development of a linear gradient SOSLC protocol was then demonstrated with a variety of samples.…”