Application of RAPD Methods in Meat for Beef Breed Identification

Choy, Yun Ho; Oh, Sung‐Jong; Kang, Ju-Yeong

doi:10.5713/ajas.2001.1655

Cited by 9 publications

(8 citation statements)

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“…3.1. RAPD analysis RAPD analysis has been successfully used in sheep genetic diversity (Kumar et al, 2008;Qasim et al, 2011) and cattle breed differentiation (Choy et al, 2001) studies. In this sense, 26 RAPD primers (Table 1) were selected from these works based on their effectiveness to distinguish breeds.…”

Section: Resultsmentioning

confidence: 99%

“…The discovery that the somatic cells present in milk may be used as source of DNA (Lipkin, Shalom, Khatib, Soller, & Friedmann, 1993), led to the development of several PCR-based techniques to allow milk origin identification in dairy products, such as Restriction Fragment Length Polymorphism (RFLP) (Abdel-Rahman & Ahmed, 2007;Fontanesi, Beretti, Dall'Olio, Portolano, Matassino & Russo, 2011), multiplex (Agrimonti, Pirondini, Marmiroli, & Marmiroli, 2015;Bottero et al, 2003;Gonçalves, Pereira, Amorim, & van Asch, 2012) and Real Time PCR (Agrimonti et al, 2015;Ganopoulos, Sakaridis, Argiriou, Madesis, & Tsaftaris, 2013;López-Calleja et al, 2007). Despite the fact that these techniques have been successfully reported for identification of milk source, the differentiation of milk origin at an intra-species level is still a major challenge (Choy, Oh, & Kang, 2001;Cirillo et al, 2012;Fontanesi, Beretti, Dall'Olio, Portolano, Matassino, & Russo, 2011;Sardina et al, 2015). Furthermore the lack of genetic information of Portuguese ovine breeds poses as a http://dx.doi.org/10.1016/j.foodchem.2016.05.109 0308-8146/Ó 2016 Elsevier Ltd. All rights reserved.…”

Section: Introductionmentioning

confidence: 99%

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RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production

Cunha

Ribeiro²,

Rocha³

et al. 2016

Food Chemistry

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Serra da Estrela Protected Designation of Origin (PDO) cheese is the most famous Portuguese cheese and has a high commercial value. However, the adulteration of production with cheaper/lower-quality milks from non-autochthones ovine breeds compromises the quality of the final product and undervalues the original PDO cheese. A Randomly Amplified Polymorphic DNA (RAPD) method was developed for efficient detection of adulterant breeds in milk mixtures used for fraudulent production of this cheese. Furthermore, Sequence Characterized Amplified Region (SCAR) markers were designed envisioning the detection of milk adulteration in processed dairy foods. The RAPD-SCAR technique is here described, for the first time, to be potentially useful for detection of milk origin in dairy products. In this sense, our findings will play an important role on the valorization of Serra da Estrela cheese, as well as on other high-quality dairy products prone to adulteration, contributing to the further development of the dairy industry.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production

Cunha

Ribeiro²,

Rocha³

et al. 2016

Food Chemistry

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“…Kemp and Teale (1994) identified population-specific DNA polymorphisms to detect Bos indicus and Bos taurus in West Africa. They estimated the allele frequency characteristics of cattle breeds using DNA markers ( Buitcamp et al, 1991 ; Mannen and Tsuji, 1993 ; Glowatzki-Mullis et al, 1995 ; Choy et al, 2001 ). Heterozygosity estimates obtained for the Burlina breed were moderate to high and consistent to the value reported by Del Bo et al (2001) that found an estimate of 0.68.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Khatun

Hossain

Rahman

2012

Asian Australas. J. Anim. Sci

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In order to develop specific genetic markers and determine the genetic diversity of Bangladeshi native cattle (Pabna, Red Chittagong) and exotic breeds (Sahiwal), randomly amplified polymorphic DNA (RAPD) analysis was performed using 12 primers. Genomic DNA was extracted from 20 cattle (local and exotic) blood samples and extracted DNA was observed by gel electrophoresis. Among the random primers three were matched and found to be polymorphic. Genetic relations between cattle’s were determined by RAPD polymorphisms from a total of 66.67%. Statistical analysis of the data, estimating the genetic distances between cattle and sketching the cluster trees were estimated by using MEGA 5.05 software. Comparatively highest genetic distance (0.834) was found between RCC-82 and SL-623. The lowest genetic distance (0.031) was observed between M-1222 and M-5730. The genetic diversity of Red Chittagong and Sahiwal cattle was relatively higher for a prescribed breed. Adequate diversity in performance and adaptability can be exploited from the study results for actual improvement accruing to conservation and development of indigenous cattle resources.

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“…Other studies conducted on RAPD-PCR for differentiation include beef of different breeds (Choy et al, 2001); animal species such as bovine, goat, pig, dog, rat, rabbit, chicken (Calvo et al, 2001b); poultry, pig, cattle including mixed meat of ostrich and cattle (Huang et al, 2003); and beef, pork, buffalo meat, mutton and chicken meat (Mane et al, 2006 and.…”

Section: Rapd-pcrmentioning

confidence: 98%

Identification of Species Origin of Meat and Meat Products on the DNA Basis: A Review

Kumar

Sharma

et al. 2013

Critical Reviews in Food Science and Nutrition

104

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The adulteration/substitution of meat has always been a concern for various reasons such as public health, religious factors, wholesomeness, and unhealthy competition in meat market. Consumer should be protected from these malicious practices of meat adulterations by quick, precise, and specific identification of meat animal species. Several analytical methodologies have been employed for meat speciation based on anatomical, histological, microscopic, organoleptic, chemical, electrophoretic, chromatographic, or immunological principles. However, by virtue of their inherent limitations, most of these techniques have been replaced by the recent DNA-based molecular techniques. In the last decades, several methods based on polymerase chain reaction have been proposed as useful means for identifying the species origin in meat and meat products, due to their high specificity and sensitivity, as well as rapid processing time and low cost. This review intends to provide an updated and extensive overview on the DNA-based methods for species identification in meat and meat products.

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Application of RAPD Methods in Meat for Beef Breed Identification

Cited by 9 publications

References 0 publications

RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production

RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production

Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Identification of Species Origin of Meat and Meat Products on the DNA Basis: A Review

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