Application of the steepest slope model reveals different perfusion territories within the mouse placenta

Remus, Chressen Catharina; Sedlacik, Jan; Wedegaertner, Ulrike; Arck, Petra C.; Hecher, Kurt; Adam, Gerhard; Forkert, Nils D.

doi:10.1016/j.placenta.2013.06.304

Cited by 19 publications

(37 citation statements)

References 22 publications

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“…Our perfusion results resulting from the separate analysis of the high-and low-flow zone mirror the characteristic vascular physiology of the mouse placenta, and are in accordance with previously reported results of maternal placental circulation [6,15]. Anatomically, the high-flow zone is located at the placental base.…”

Section: Discussionsupporting

confidence: 91%

“…The standard deviation can be substantially reduced by analysing the high-and low-flow compartment separately as shown in this work. The average perfusion values found in this study were significantly different between the high-and low-flow compartment and in the range of previously reported values (highflow: 158 ± 58 ml/min/100 g; low-flow: 114 ± 52 ml/min/100 g [6]). …”

Section: Discussioncontrasting

confidence: 73%

“…However, recent study results suggest that perfusion differs significantly between different regions of the placenta [6,15]. Many pregnancy complications such as fetal growth restriction and highrisk pregnancies have been associated with reduced maternal blood flow to the placenta [2].…”

Section: Discussionmentioning

confidence: 99%

“…The goldstandard compartment definitions (high-vs. low-flow) were independently defined by the two observers following the procedure described by Remus et al [6]. In brief, the high-flow compartment was defined as the tissue with visible contrast enhancement in the second image after contrast agent arrival.…”

Section: Evaluation and Statistical Analysismentioning

confidence: 99%

“…It was recently shown that two placental compartments e a high-and a low-flow compartment e can be differentiated using DCE MRI datasets by visually analysing the gradual filling of the intervillous space [5,6]. More precisely, the two perfusion compartments were interactively defined in both studies by drawing regions-of-interest (ROIs) based on the contrast agent distribution at different time points.…”

Section: Introductionmentioning

confidence: 99%

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Automatic differentiation of placental perfusion compartments by time-to-peak analysis in mice

et al. 2015

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Evaluation and Statistical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Automatic differentiation of placental perfusion compartments by time-to-peak analysis in mice

et al. 2015

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Optimized time‐resolved imaging of contrast kinetics (TRICKS) in dynamic contrast‐enhanced MRI after peptide receptor radionuclide therapy in small animal tumor models

Haeck

Bol

Bison

et al. 2015

Contrast Media & Molecular

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Anti-tumor efficacy of targeted peptide-receptor radionuclide therapy (PRRT) relies on several factors, including functional tumor vasculature. Little is known about the effect of PRRT on tumor vasculature. With dynamic contrast-enhanced (DCE-) MRI, functional vasculature is imaged and quantified using contrast agents. In small animals DCE-MRI is a challenging application. We optimized a clinical sequence for fast hemodynamic acquisitions, time-resolved imaging of contrast kinetics (TRICKS), to obtain DCE-MRI images at both high spatial and high temporal resolution in mice and rats. Using TRICKS, functional vasculature was measured prior to PRRT and longitudinally to investigate the effect of treatment on tumor vascular characteristics. Nude mice bearing H69 tumor xenografts and rats bearing syngeneic CA20948 tumors were used to study perfusion following PRRT administration with (177) lutetium octreotate. Both semi-quantitative and quantitative parameters were calculated. Treatment efficacy was measured by tumor-size reduction. Optimized TRICKS enabled MRI at 0.032 mm(3) voxel size with a temporal resolution of less than 5 s and large volume coverage, a substantial improvement over routine pre-clinical DCE-MRI studies. Tumor response to therapy was reflected in changes in tumor perfusion/permeability parameters. The H69 tumor model showed pronounced changes in DCE-derived parameters following PRRT. The rat CA20948 tumor model showed more heterogeneity in both treatment outcome and perfusion parameters. TRICKS enabled the acquisition of DCE-MRI at both high temporal resolution (Tres ) and spatial resolutions relevant for small animal tumor models. With the high Tres enabled by TRICKS, accurate pharmacokinetic data modeling was feasible. DCE-MRI parameters revealed changes over time and showed a clear relationship between tumor size and Ktrans .

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Placental perfusion in uterine ischemia model as evaluated by dynamic contrast enhanced MRI

Drobyshevsky¹,

Prasad

2015

Magnetic Resonance Imaging

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Background To validate DCE MRI method of placental perfusion estimation and to demonstrate application of the method in a rabbit model of fetal antenatal hypoxia-ischemia. Methods Placental perfusion was estimated by dynamic contrast imaging with bolus injection of Gd-DTPA in 3 Tesla GE magnet in a rabbit model of placental ischemia–reperfusion in rabbit dams at embryonic day 25 gestation age. Placental perfusion was measured using steepest slope method on DCE MRI before and after intermittent 40 min uterine ischemia. Antioxidants (n = 2 dams, 9 placentas imaged) or vehicle (n = 5 dams, 23 placenta imaged) were given systemically in a separate group of dams during reperfusion–reoxygenation. Placental perfusion was also measured in two dams from the antioxidant group (10 placentas) and two dams from the control group (12 placentas) by fluorescent microspheres method. Results While placental perfusion estimates between fluorescent microspheres and DCE MRI were significantly correlated (R2 = 0.85; P < 0.01), there was approximately 33% systematic underestimation by the latter technique. DCE MRI showed a significant decrease in maternal placental perfusion in reperfusion–reoxygenation phase in the saline, 0.44 ± 0.06 mL/min/g (P = 0.012, t-test), but not in the antioxidant group, 0.62 ± 0.06 mL/min/g, relative to preocclusion values (0.77 ± 0.07 and 0.84 ± 0.12 mL/min/g, correspondingly). Conclusion Underestimation of true perfusion in placenta by steepest slope DCE MRI is significant and the error appears to be systematic.

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Application of the steepest slope model reveals different perfusion territories within the mouse placenta

Cited by 19 publications

References 22 publications

Automatic differentiation of placental perfusion compartments by time-to-peak analysis in mice

Automatic differentiation of placental perfusion compartments by time-to-peak analysis in mice

Optimized time‐resolved imaging of contrast kinetics (TRICKS) in dynamic contrast‐enhanced MRI after peptide receptor radionuclide therapy in small animal tumor models

Placental perfusion in uterine ischemia model as evaluated by dynamic contrast enhanced MRI

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