Application of two rapid techniques of column chromatography to separate the pungent principles of ginger,Zingiber officinale Roscoe

Desenvolvimento de Cerveja Artesanal de Trigo Adicionada de Gengibre (Zingiber Officinale Roscoe)

“…As plantas da família Zingiberaceae, em geral, apresentam ingredientes pungentes. O gengibre apresenta compostos como 6-gingerol e 6-paradol, os quais possuem efeito antitumoral (Figura 1.2) (ZARATE; SUKRASNO; YEOMAN, 1992). Segundo Lee, Kim e Ashmore (1985), o gengibre demonstrou propriedade antioxidante, conferindo aumento de vida útil de produtos alimentícios.…”

Effect of isolated fractions of Harpagophytum procumbens D.C. (devil's claw) on COX‐1, COX‐2 activity and nitric oxide production on whole‐blood assay

“…The crude hydroalcohol extract of H. procumbens (5 g) was fractionated by a short column vacuum chromatographic method (fl ash chromatography, FC) using a normal phase silica bed 60 (200-500 mm, Merck) and a mixture of chloroform/methanol (CHCl 3 :CH 3 OH, 200 mL, ratio 100.0% chloroform, 99:01,98:02, 96:04, 92:08, 90:10, 88:12, 85:15 and 80:20, 70:30, 50:50 and 100% methanol) to elute the components, it resulted in 12 fractions (F1-F12), (Zarate et al, 1992). The 12 fractions obtained were monitored by thin-layer chromatography (TLC silica gel 60 F 254 Merck) eluted with a mixture of CHCl 3 :CH 3 OH (75:25 v/v).…”

Harpagoside suppresses lipopolysaccharide-induced iNOS and COX-2 expression through inhibition of NF-κB activation

“…The extract was fractionated through the normal phase silica bed using a short column vacuum chromatographic method (Zarate et al, 1992). A stepwise gradient of increasing polarity of mobile phase (200 ml) consisting of a mixture of chloroform/methanol (CHCl 3 :MeOH ratio; 100:0, 99:1, 98:2, 96:4, 94:6, 92:8, 90:10, 88:12, 85:15 and 80:20) was employed to elute the components.…”