Arabidopsis ANGUSTIFOLIA3 (AN3) is associated with the promoter of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) to regulate light‐mediated stomatal development

Abstract

Light signals are perceived by multiple photoreceptors that converge to suppress the RING E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) for the regulation of stomatal development. Thus, COP1 is a point of integration between light signaling and stomatal patterning. However, how light signaling is collected into COP1 for the production and spacing of stomata is still unknown. Here, we report that the loss-of-function mutant of ANGUSTIFOLIA3 (AN3) delays asymmetric cell division, which leads to decreased stomatal index. Furthermore, overexpression of AN3 accelerates asymmetric cell division, which results in clusters of stomata. In addition, the stomatal development through AN3 regulation is mediated by light signaling. Finally, we find that an3 is a light-signaling mutant, and that AN3 protein is light regulated. Self-activation by AN3 contributes to the control of AN3 expression. Thus, AN3 is a point of collection between light signaling and stomatal patterning. Target-gene analysis indicates that AN3 is associated with COP1 promoter for the regulation of light-controlling stomatal development. Together, these components for regulating stomatal development form an AN3-COP1-E3 ubiquitin ligase complex, allowing the integration of light signaling into the production and spacing of stomata.

Sucrose Signaling Regulates Anthocyanin Biosynthesis Through a MAPK Cascade in Arabidopsis thaliana

“…Total RNA was extracted from tissues indicated in the figures by the TRIzol reagent (Invitrogen, Carlsbad, CA), as has been described by Meng and Yao (2015) and Meng et al (2018). SYBR green was used to monitor the kinetics of PCR product in real-time RT-PCR, as has been described by Meng and Yao (2015).…”

The Tomato Genome Encodes SPCH, MUTE, and FAMA Candidates That Can Replace the Endogenous Functions of Their Arabidopsis Orthologs

“…The molecular components regulating this process have been studied in depth in the model species Arabidopsis thaliana , providing insight into the networks that control the genetic program of stomatal development (Bergmann and Sack, 2007; Pillitteri and Torii, 2012; Simmons and Bergmann, 2016; recently reviewed by Qu et al, 2017). The links of this program to environmental factors such as light, temperature, atmospheric CO 2 , or water availability have also been explored (Hetherington and Woodward, 2003; Casson and Grey, 2008; Lake and Woodward, 2008; Franks and Beerling, 2009; Casson and Hetherington, 2010; Tricker et al, 2012; Lee et al, 2017; Lau et al, 2018; Meng et al, 2018; Qi and Torii, 2018). The present scenario describes a complex network where membrane receptors and signaling peptides act through protein kinase cascades (to prevent too many stomata and ensure that they are flanked by the needed subsidiary cells to function), modulating the activity of positive drivers of stomata development, the outcome of these opposed regulators determining stomatal abundance and distribution on the leaf epidermis.…”

Glucose- and sucrose-signaling modules regulate the Arabidopsis juvenile-to-adult phase transition

“…Transgenic lines containing 35S:PAP1:HA/myb75-1 were used in this experiment. ChIP was performed using 15 day-old seedlings grown in short days (Meng et al, 2015a(Meng et al, , 2015b(Meng et al, , 2016(Meng et al, , 2018a(Meng et al, , 2018b. Please see Table S1 for details of specific primers.…”