Articular cartilage preservation and storage

Brighton, Carl T.; Shadle, Chester A.; Jimenez, Sergio A.; Irwni, John T.; Lane, Joseph M.; Lipton, Mitchel

doi:10.1002/art.1780221008

Cited by 50 publications

(3 citation statements)

References 22 publications

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“…An increase in shelf life will have a very significant impact on the treatment of cartilage lesions by expanding the availability of osteochondral allografts. While Brighton and co-workers showed promising findings using tissue culture techniques for cartilage maintenance nearly two decades ago, cold storage ($4 1C) is the current standard for osteochondral graft preservation and storage (Brighton et al, 1979). In the current study, we revisit the potential of using in vitro techniques for maintaining cartilage explants in long-term culture.…”

Section: Introductionmentioning

confidence: 98%

Mechanical and biochemical characterization of cartilage explants in serum-free culture

Bian¹,

Lima²,

Angione³

et al. 2008

Journal of Biomechanics

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Allografts of articular cartilage are both used clinically for tissue-transplantation procedures and experimentally as model systems to study the physiological behavior of chondrocytes in their native extracellular matrix. Long-term maintenance of allograft tissue is challenging. Chemical mediators in poorly defined culture media can stimulate cells to quickly degrade their surrounding extracellular matrix. This is particularly true of juvenile cartilage which is generally more responsive to chemical stimuli than mature tissue. By carefully modulating the culture media, however, it may be possible to preserve allograft tissue over the long-term while maintaining its original mechanical and biochemical properties. In this study juvenile bovine cartilage explants (both chondral and osteochondral) were cultured in both chemically defined medium and serum-supplemented medium for up to 6 weeks. The mechanical properties and biochemical content of explants cultured in chemically defined medium were enhanced after 2 weeks in culture and thereafter remained stable with no loss of cell viability. In contrast, the mechanical properties of explants in serum-supplemented medium were degraded by ( approximately 70%) along with a concurrent loss of biochemical content (30-40% GAG). These results suggest that long-term maintenance of allografts can be extended significantly by the use of a chemically defined medium.

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Section: Introductionmentioning

confidence: 98%

Mechanical and biochemical characterization of cartilage explants in serum-free culture

Bian¹,

Lima²,

Angione³

et al. 2008

Journal of Biomechanics

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“…Brighton et al [3] and Black et al [21 were the first to report extensive studies on the application of tissue culture techniques to cartilage preservation. They observed that cartilage slices stored in tissue culture medium at 37 ° C survived for as long as 60 days.…”

Section: Discussionmentioning

confidence: 99%

Prolonged vital cartilage graft preservation using tissue culture methods

et al. 1994

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Cartilage grafting is one of the most commonly performed procedures in plastic surgery. Since storage of both autologous and allogenic cartilage is necessary, different preservation methods have been used with varying success. The use of chemical preservation procedures such as formaldehyde, Merthiolate or Cialit lead to a loss of viability of the graft. This work presents a study of the cell viability of cartilage grafts stored in different solutions (saline, RPMI 1640 and DMEM) for 100 days. Cartilage immersed in saline solution lost 50% of its viability after a storage period of 6 days, after 30 days no viable cells could be observed. In contrast to this, cartilage immersed in tissue culture media retained its viability (> 80%) during the whole storage period. No differences were found between either culture medium. The results of these experiments suggest that viable cartilage tissue can be successfully stored over a long period of time using tissue culture procedures.

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“…Previous studies have shown variable results regarding the effect of storage on graft quality (Viidik and Lewin 1966;Brighton 1979;Woo et al 1986;Schachar et al 1994;Oates et al 1995;Smith et al 1996;Sammarco et al 1997;Williams et al 2003;Rohde et al 2004;Williams et al 2005;Malinin et al 2006;Pennock et al 2006;Lightfoot et al 2007;Williams et al 2007;Giannini et al 2008). Optimum refrigeration times for osteochondral allografts have been reported in the literature to be less than 5-7 days (Schachar et al 1994;Rohde et al 2004) or as long as 42 days (Williams et al 2007).…”

Section: Introductionmentioning

confidence: 89%

Storage conditions do not have detrimental effect on allograft collagen or scaffold performance

2009

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Musculoskeletal allografts are a valuable alternative to autograft tissue in orthopaedic surgeries. However, the effects of the allografts' storage history on the collagen and subsequent allograft scaffold properties are unknown. In this study, we hypothesized that freezing and refrigeration of allografts for 1 week would alter the biologic performance and mechanical properties of the allograft collagen. Allograft collagen was characterized by SDS-PAGE migration pattern, amino acid profile and measured denaturation. Scaffolds made from allograft collagen were evaluated for fibroblast proliferation, platelet activation and scaffold retraction. Collagen gelation kinetics (elastic and inelastic moduli and the viscous-elastic transition point) were also evaluated. Fibroblast proliferation, platelet activation and scaffold retraction results showed only minor, though statistically significant, differences between the storage groups. In addition, there were no significant differences in rheological properties or collagen biochemistry. In conclusion, this study suggests that freezing or refrigeration for 1 week does not appear to have any detrimental effect on the mechanical properties and biologic performance of the collagen within allografts.

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Articular cartilage preservation and storage

Cited by 50 publications

References 22 publications

Mechanical and biochemical characterization of cartilage explants in serum-free culture

Mechanical and biochemical characterization of cartilage explants in serum-free culture

Prolonged vital cartilage graft preservation using tissue culture methods

Storage conditions do not have detrimental effect on allograft collagen or scaffold performance

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