Assessing the robustness of IRAP and RAPD marker systems to study intra-group diversity among Cavendish (AAA) clones of banana

Saraswathi, M.; Uma, S.; Selvam, Kanichelvam Prasanya; Ramaraj, S.; Durai, P.; Mustaffa, M. M.

doi:10.1080/14620316.2011.11512717

Cited by 14 publications

(11 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, 'Atiakol', having 'BB' genotype could be the ancestor of Group-II with 'ABB' or 'BBA' genotypes. Saraswathi et al [3] reported intra-group diversity among Cavendish (AAA) clones of banana using IRAP and RAPD marker systems which was in accordance with the present findings. The amplification of microsatellites could be tested at different chromosomal sites in between diverging species, and events of differential deletions in evolving species could follow microsatellite amplifications in a common ancestor [40,41].…”

Section: Phylogenetic Analysissupporting

confidence: 96%

“…North-eastern India possessed more wild germplasms with 'BB' rich genomes, disease resistant genotypes, which could be the potential diploid donors for developing disease resistance varieties through breeding. The RAPD and IRAP are stable DNA-based marker techniques that have been successfully used to distinguish 'A' and 'B' genomes to indentify Musa germplasms as well as provide useful information to elucidate genetic relationships [3]. The exploitation of plant genes and genomes for plant breeding is now becoming better recognized particularly after the publication of banana genomic map [4].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Genetic Diversity in 21 Genotypes of Indian Banana Using RAPDs and IRAPs Markers

Shelke

Das

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

View full text Add to dashboard Cite

Abstracts Table top banana (AAA) and cooking type plantines (BBB/ABB) are cultivated commercially for high yield and lucrative market value. Morphological similarities among the banana clones make identification difficult under field conditions. Random amplified polymorphic DNA (RAPD) and inter-retrotransposon amplified polymorphism (IRAP), were used to characterize genetic variations among 21 banana germplasm. IRAP primers were designed to determine 'AA' and 'BB' specific markers on the basis of repetitive and genome-wide dispersed long terminal repeat (LTR) retrotransposons. RAPD markers successfully detected genetic variation between genotypes. IRAP markers amplified either by a single primer or a combination of primers, based on LTR orientation, successfully amplified different retrotransposons dispersed in the Musa genome. The average level of polymorphism exhibited by RAPD and IRAP markers were 71.47 % and 81.3 % respectively that suggests substantial genetic variations among the tested varieties. All the 12 table-top varieties were clustered together while four cooking varieties i.e. Bantala-I, Bantala-II, Dakhkhnisagar and Athiakol with 'BB' formed a distinct group. These results suggested that IRAP markers were found more robust than RAPD markers to study the intra-group genetic diversity between table top and cooking banana.

show abstract

Section: Phylogenetic Analysissupporting

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Diversity in 21 Genotypes of Indian Banana Using RAPDs and IRAPs Markers

Shelke

Das

2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…In parallel, several studies have also demonstrated the utility of various classes of DNA markers in Musa spp. (Ude et al, 2002;Nwakanma et al, 2003;Buhariwalla et al, 2005;Teo et al, 2005;Heslop-Harrison & Schwarzacher, 2007;Garcia et al, 2010;Saraswathi et al, 2011;Ravishankar et al, 2013;Brown et al, 2017). Currently, a form of marker-selection known as genomic selection is being developed for use in generating appropriate breeding models for the improvement of banana (Nyine et al, 2017).…”

Section: Understanding the Host Response To Pseudocercospora Banana Pmentioning

confidence: 99%

Progress in understanding Pseudocercospora banana pathogens and the development of resistant Musa germplasm

et al. 2018

View full text Add to dashboard Cite

Banana and plantain (Musa spp.) are important food crops in tropical and subtropical regions of the world where they generate millions of dollars annually to both subsistence farmers and exporters. Since 1902, Pseudocercospora banana pathogens, Pseudocercospora fijiensis, P. musae and P. eumusae, have emerged as major production constraints to banana and plantain. Despite concerted efforts to counter these pathogens, they have continued to negatively impact banana yield. In this review, the economic importance, distribution and the interactions between Pseudocercospora banana pathogens and Musa species are discussed. Interactions are further scrutinized in the light of an emerging climate change scenario and efforts towards the development of resistant banana germplasm are discussed. Finally, some of the opportunities and gaps in knowledge that could be exploited to further understanding of this ubiquitous pathosystem are highlighted.

show abstract

“…Electrophoresis was conducted at 50-60 V for 3 h and the gel were photographed under UV light using a gel dock system. (Saraswathi, 2011).…”

Section: Visualization Of Dna In Agarose Gel:-mentioning

confidence: 99%

Analysis of Genetic Variation in Different Banana Variety (Musa) Species by Random Amplified Polymorphic Dna.

Karunambika¹,

Devi²,

Begum³

2018

IJAR

View full text Add to dashboard Cite

Assessing the robustness of IRAP and RAPD marker systems to study intra-group diversity among Cavendish (AAA) clones of banana

Cited by 14 publications

References 1 publication

Analysis of Genetic Diversity in 21 Genotypes of Indian Banana Using RAPDs and IRAPs Markers

Analysis of Genetic Diversity in 21 Genotypes of Indian Banana Using RAPDs and IRAPs Markers

Progress in understanding Pseudocercospora banana pathogens and the development of resistant Musa germplasm

Analysis of Genetic Variation in Different Banana Variety (Musa) Species by Random Amplified Polymorphic Dna.

Contact Info

Product

Resources

About