Assessment and Separation of Angiotensin I-Converting Enzyme Inhibitory Peptides in Chinese Soypaste

The renin-angiotensin-aldosterone system is a signaling pathway which responsible in the blood pressure regulation. Angiotensin-converting enzyme (ACE) is one of the key elements responsible for the hypertensive mechanism. It converts angiotensin-I to angiotensin-II. The discovery history of the ACE inhibitory activity assay method has been through a long stage for decades and development continues until today. The ACE inhibitory activity has become an effective screening method in the search for new antihypertensive agents from herbal plants. Some of in vitro assay methods were used to examine the activity of ACE inhibitors based on the substrate usage, such as; Cushman and Cheung Method using a substrate hippuryl-histidyl-leucine (HHL), Holmquist method using a substrate furanacryloyl-tripeptide, Elbl and Wagner method using a substrate benzoil-[l-14C] glicyl-L-histidine-L-leucine, Carmel and Yaron method using a substrate o-aminobenzoylglycyl-p-nitrophenylalanilproline, and Lam method using 3-hydroxybutyrylglycyl-glycyl-glycine as substrate. Several different methods to measure the results of enzymatic reactions or separating substrate with products, including spectrophotometric, fluorometric, high-performance liquid chromatography, electrophoresis, and radiochemistry. Application of the test method for screening the ACE inhibitors activity and investigation of active compounds from natural products can be done easily with this method, it is very helpful in research because the results obtained are simple, accurate, and rapid.

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“…The LAM method with the 3HB-GGG substrate using the ACE kit-WST can also be applied using microplate ELISA reader. [ 48 49 50 ]…”

Section: Resultsmentioning

confidence: 99%

Review of angiotensin-converting enzyme inhibitory assay: Rapid method in drug discovery of herbal plants

Ahmad¹,

Yanuar²,

Mulia³

et al. 2017

Phcog Rev

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“…These data suggested that both PKH3 and PKH4 could bind to ACE non-active sites as well as to the enzymesubstrate complex (Asoodeh et al, 2014). Similarly, peptides from Chinese soy paste (Li et al, 2015) and flaxseed protein peptides (Udenigwe et al, 2009) were non-competitive ACE inhibitors. Moreover, competitive type (Setayesh-Mehr & Asoodeh, 2017) and mixed-type (Wang et al, 2018) of ACE inhibitory mechanisms have been reported as well.…”

Section: Pkhs Inhibit Ace In a Non-competitive Waymentioning

confidence: 92%

Angiotensin converting enzyme inhibitory peptide fractions from Tibet wild peach kernel protein hydrolysates

Yang

Zhong

et al. 2019

Acta Alimentaria

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In this study, the peach kernel proteins were extracted and treated with alkaline proteinase to generate peach kernel protein hydrolysate (PKH), which showed angiotensin converting enzyme (ACE) inhibition activity. The hydrolysate was separated into four fractions and their anti-ACE activities were investigated. Our results showed that all PKHs had anti-ACE activity, and the lowest molecular weight fraction PKH4 had the highest ACE inhibitory activity. Lineweaver-Burk plots illustrated that the inhibition types of PKH3 and PKH4 were non-competitive. The K i of PKH4 was lower than K i of PKH3; suggesting PKH4 had high affinity to ACE. Amino acid composition analysis showed that the best anti-ACE peptide PKH4 possessed high levels of hydrophobic amino acids, branched-chain amino acids, and aromatic amino acids. In summary, our findings demonstrated that high anti-ACE activity is negatively related to the size of the PKHs and possibly the composition of amino acids, and the PKH4 was the best ACE inhibitor. Further, peach kernel peptides can be developed as a functional food for patients with hypertension.

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Bioactive peptides derived from traditional Chinese medicine and traditional Chinese food: A review

Liu

Wang

Liu

et al. 2016

Food Research International

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Assessment and Separation of Angiotensin I-Converting Enzyme Inhibitory Peptides in Chinese Soypaste

Cited by 5 publications

References 24 publications

Review of angiotensin-converting enzyme inhibitory assay: Rapid method in drug discovery of herbal plants

Review of angiotensin-converting enzyme inhibitory assay: Rapid method in drug discovery of herbal plants

Angiotensin converting enzyme inhibitory peptide fractions from Tibet wild peach kernel protein hydrolysates

Bioactive peptides derived from traditional Chinese medicine and traditional Chinese food: A review

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