Assessment genetic variation and relationship of sago palm (Metroxylon sagu Rottb.) in Indonesia based on specific expression gene (Wx genes) markers

Abbas, Barahima

doi:10.5897/ajps12.015

Cited by 10 publications

(19 citation statements)

References 25 publications

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“…A total of 8 polymorphic alleles and 14 genotypes of Wx genes were observed in the sago palm genome. Alleles ranging from 100 to 700 bp were detected on agarose gels (Abbas and Ehara 2012). Polymorphism levels detected in sago palm were similar to those found in the wheat (Triticum aestivum L.) genome by Shariflou and Sharp (1999), using a Wx gene (Sun1) primer.…”

Section: Waxy Gene Markerssupporting

confidence: 75%

Sago Palm

Ehara¹,

Toyoda²,

Johnson³

2018

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Section: Waxy Gene Markerssupporting

confidence: 75%

Sago Palm

Ehara¹,

Toyoda²,

Johnson³

2018

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Section: Waxy Gene Markerssupporting

confidence: 66%

Sago Palm Genetic Resource Diversity in Indonesia

Abbas

2018

Sago Palm

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Information of genetic diversity is very important in supporting the implementation of genetic resource conservation and plant breeding. This study aims to determine the genetic diversity of 11 sago palm accessions that have been collected by the Sago Research Consortium (SRC), University of Papua (UNIPA). Methods of research were divided into three steps.Step 1 was DNA extraction by using Geneaid DNA plant Mini Kit.Step 2 was PCR amplification fragments DNA by using KAPA2G Robust HotStart polymerases and performed by using Bio-Rad PCR instrument.Step 3 was purified and sequenced DNA by Macrogen Inc. Seoul, Korea. Data analyses were performed by using a MEGA6.06 software. Morphological characteristics in the rosette stage of 11 sago palm accessions were divided into 4 phenotypes only, and several accessions showed the same phenotypes. Based on atp6-2 gene markers, the 11 sago palm accessions had a different genetic characteristic among the others. Genetic distances among 11 sago palm accessions were in the range among 0.22 and 3.01 with an average 1.21 pairwise distant. Phylogenetic construction showed that the genetic relationship of 11 sago palm accessions was clustered into 6 groups. The accession numbers SP001 and SP002 are in group 1, the accession numbers SP003 and SP011 group 2, the accession numbers SP005 and SP007 group 3, the accession number SP009 group 4, the accession numbers SP008 and SP010 group 5, and the accession numbers SP004 and SP006 group 6. Nucleotide sequence alignments of mitochondrial atp6-2 gene and introns were different from each other in 11 accessions.

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“…Primers OPAW05, OPA01, and OPA04 were observed overall amplification DNA fragments it shown polymorphic whereas, primer P01 was resulted 3 DNA fragments polymorphic and 5 DNA fragments monomorphic and primer OPD08 was resulted 7 DNA fragment polymorphic and 2 DNA fragments monomorphic. Amplification of DNA sago palm by using those primers set was consistently resulting high polymorphism [16][17][18]. The polymorphisms of DNA fragments product by using RAPD markers were related to the variation of morphological phenotypic.…”

Section: Rapd Polymorphismmentioning

confidence: 99%

“…Sample S01 and S10 were genetically large differences. The previous study were reported that the sago palm in the natural habitat show a lot of variation based on morphological characteristic [19], growth and yield production [20] Chloroplast DNA [17], and specific expression gene [18]. Analysis of phylogenetic trees based on the pattern of polymorphism of RAPD markers was done by using UPGMA method.…”

Section: Genetic Variationsmentioning

confidence: 99%

Genetic Variation of Sago Palm (Metroxylonsagu Rottb.) Progenies with Natural Pollination by Using RAPD Markers

Abbas¹,

Dailami²,

Santoso³

et al. 2017

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Sago palm is flowering and fruiting just once in their life cycle. Sago palms that grow naturally and semi cultivated were generally occurred natural pollination to form fruits and seeds, if not cut down to take the starch contained in their trunk. Sago palm pollination may occur as self-pollinated and cross-pollinated. If cross-pollinated was occurred in the pollination process, it will be varied of their progenies. This study aims to reveal the genetic variation of sago palms progenies with naturally pollinated process. The research method is to collect seeds from one parent trees that have produced ripe fruit. Fruit seeds germinated to be made and tested genetic variation using RAPD markers. Isolation of DNA is done by using the fresh young leaves. DNA amplification is done by using RAPD primers. The results showed that the progenies derived from naturally pollinated of sago palms were genetically varied based on RAPD markers and also varied based on morphological phenotypic. Variations occurred in the progenies of sago palm indicated that the sago palms were estimated cross-pollinated naturally, as a result fruits and seeds with genetically differences.

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Assessment genetic variation and relationship of sago palm (Metroxylon sagu Rottb.) in Indonesia based on specific expression gene (Wx genes) markers

Cited by 10 publications

References 25 publications

Sago Palm

Sago Palm

Sago Palm Genetic Resource Diversity in Indonesia

Genetic Variation of Sago Palm (Metroxylonsagu Rottb.) Progenies with Natural Pollination by Using RAPD Markers

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