Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

Abstract

The aim of this study is designed to evaluate the best methods for cow oocytes collection from abattoir specimens which is the cheapest, easily obtained and bulky number. Forty five fresh cow genitalia specimens and testicle were collected directly after slaughter from Al-Shoáalla abattoir northwest of Baghdad the capital early morning, transported in cool box under (4-8) °C to the laboratory of theriogenology in the college of veterinary medicine/Baghdad University during the period from November 2016 to February 2017. Ovaries were separated from the surrounding tissues, washed thoroughly with dis. water repeatedly, then with normal saline and finally with MEM medium containing Antibiotics and Nystatin for contaminant elimination. Oocytes were collected with four methods aspiration, slashing, slicing after aspiration and slicing. The result showed that; the collected oocytes were 55, 68, 87 and 106 oocytes respectively; slicing methods yield more oocytes count. Period of time between slaughtering and samples processing significantly affect oocytes collected percentage and quality, periods as 2, 6, 12 and 24 hours yield 75%, 68%, 61% and 55% oocytes counts of good, fair, poor to aged and bad quality oocytes respectively. Two hours period yield an elevated oocytes count with good quality. Maturation index of oocytes according to the type of collected methods showed 44, 37, 39 and 42 with 12, 8, 6 and 6 good oocyte quality for the four methods respectively. In conclusion slicing methods yield more oocytes count with a moderate quality and embryos production while aspiration methods yield a moderate oocytes count with an elevated quality and good embryos production.

Evaluation of Different Techniques in Recovering of Oocytes and Storage Duration of Ovaries on the Quality and Quantity of Bovine in Vitro Maturation

“…This result was similar to Abdel-Khalek et al (2010) that reported the recovery rate was in significant decrease from 68.1% at 5 h to 78.5% at 9 h of preservation. However, it is better to transport the specimen from slaughterhouse to the place of processing directly after slaugther if preserved well by cool box under 4-8ºC (Saleh, 2017). The result of this study was higher than that of Saleh (2017) who reported a recovery rate of 75% at 2 h and 55% at 24 h, and Abdel- Khalek et al (2010) who reported a range from 9.9 to 12.4 oocyte per ovary.…”

“…However, it is better to transport the specimen from slaughterhouse to the place of processing directly after slaugther if preserved well by cool box under 4-8ºC (Saleh, 2017). The result of this study was higher than that of Saleh (2017) who reported a recovery rate of 75% at 2 h and 55% at 24 h, and Abdel- Khalek et al (2010) who reported a range from 9.9 to 12.4 oocyte per ovary. The different of oocytes quantity is due to several factors such as the reproductive status, age and the the collection methods of oocytes.…”

“…In cattle ovary storage at 37ºC for 8 h significantly decreased both rates of cleavage and blastocyst formation after IVM/ IVF oocytes (2). According to Saleh (2017) that period between slaughtering and sample processing significantly affect oocytes collected percentage and quality. Bacelo-Fimbres et al (2015) founded that shipping slaughterhouse derived bovine oocytes either for 18 hours or for 24 hours was superior to conventional maturation system for blastocyst production at day 7 of IVC.…”

“…Depending on the efficiency of aspiration and the oocyte grading system, the oocyte harvest per ovary can range from 0.46 to 3.0, with an average of 1.5 per ovary (Totey et al, 1992), but the limitation of oocyte collection from slaughtered ovaries is that no information about pedigree of dam is obtained. According to Saleh (2017), slicing the cow ovaries methods for oocytes collection yields more collected oocytes count with moderate quality while aspiration methods yield fewer oocyte numbers with good quality.…”

Utilization of Ovary Collected from Abattoir as Genetic Material Resources

“…Sumber: Saleh (2017) Teknik lain dalam koleksi oosit adalah teknik penyayatan (slicing). Ovarium ditempatkan pada cawan petri yang telah diberi 5 ml medium koleksi dan ovarium ditahan menggunakan pinset.…”