Assessment of insecticide resistance in primary dengue vector, Aedes aegypti (Linn.) from Northern Districts of West Bengal, India

Bharati, Minu; Saha, Dhiraj

doi:10.1016/j.actatropica.2018.07.004

Cited by 20 publications

(9 citation statements)

References 30 publications

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“…quinquefasciatus by the accumulation of malathion used in agricultural sector leads to indirect exposure of mosquitoes and in turn to the development of resistance against the same. Similar findings of resistance development to agriculturally sprayed malathion in Aedes aegypti population of this region has already been reported by Bharati and Saha (2018) [22]. Furthermore, there are similar reports on the development of resistance by field populations of Cx .…”

Section: Discussionsupporting

confidence: 88%

“…Likewise, low mortality rates suggesting severe resistance to malathion in ISL and FLB may also be coupled with enhanced activity of both α-CCEs and β-CCEs of these populations among all other tested populations (Tables 4 and 5). Associations of similar kind in organophosphate resistant mosquitoes to elevated levels of non specific CCEs have been reported around the globe [22, 25–26]. Amplification of non specific esterases is the most frequently observed resistance mechanism in organophosphate resistant populations of Culex and Aedes [27].…”

Section: Discussionmentioning

confidence: 99%

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Insecticide resistance mapping in the vector of lymphatic filariasis, Culex quinquefasciatus Say from northern region of West Bengal, India

et al. 2019

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Culex quinquefasciatus is a vector of lymphatic filariasis and vector control strategies normally involve the use of synthetic insecticides targeted against them. Extensive and uncontrolled use of these synthetic insecticides has led to the development of insecticide resistance in the mosquito vectors. In this context, to study the resistance status of Cx . quinquefasciatus , field populations were collected from three districts of Northern part of West Bengal and tested against insecticides (5% malathion, 0.05% deltamethrin, 0.05% lambdacyhalothrin,0.75% permethrin, 0.1% propoxur, 4% DDT and Temephos). Qualitative and quantitative enzyme assay was also conducted in order to find the role of detoxifying enzymes behind the development of insecticide resistance. This study revealed the presence of widespread resistance amongst the field populations of Cx . quinquefasciatus throughout the studied regions. Moreover, the result of native PAGE and biochemical enzyme assay may be linked to some extent in the involvement of the detoxifying enzymes in conferring resistance against insecticides in most of the tested Cx . quinquefasciatus populations. The present study involving the survey of resistance status may be of immense help during the implementation of vector control strategies throughout this region.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Insecticide resistance mapping in the vector of lymphatic filariasis, Culex quinquefasciatus Say from northern region of West Bengal, India

et al. 2019

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“…Several reports are available on insecticide resistance status of the dengue vector [30, 31, 32, 33, 34] from India, but such reports from West Bengal are very rare particularly for Ae . aegypti [35]. A regular monitoring of insecticide resistance and studies on mechanisms behind it are very important to detect the effectiveness of the used insecticides and newer ones against the prevailing vector population of any geographical region.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of kdr mutations and insecticide susceptibility among natural population of Aedes aegypti in West Bengal

Saha¹,

Chatterjee²,

Ballav³

et al. 2019

PLoS ONE

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Background Aedes albopictus and Aedes aegypti are the major vectors of arboviral diseases. As effective vaccines are not available for most of the arboviral diseases, vector control by using insecticides play the key role to reduce the disease transmission. The emergence and spread of resistance to different classes of insecticides by the vectors is a major obstacle to control the disease transmission. Information about vector susceptibility to different insecticides and their mechanisms are very important for formulating proper vector control measures. The present study was designed to assess the susceptibility of Ae . aegypti against three different classes of adulticides, one larvicidal agent available and polymorphisms in the voltage-gated sodium channel ( VGSC ) gene related to insecticide resistance. Methods Immature stages of Ae . aegypti were collected from three dengue endemic municipal areas of West Bengal and reared in the laboratory. Larvae and adults (F1 progeny) were used for insecticide bioassay as per WHO protocols. Knock down resistance gene ( kdr ) mutations were assessed by direct sequencing of PCR products. Results The Ae . aegypti population was found to be susceptible to type II pyrethroids and malathion but highly resistant to DDT. A high rate of polymorphisms in the VGSC gene was observed among the collected mosquitoes. A double mutant V1016 G + F1534 C was found to be associated with DDT resistance but neither V1016 G nor F1534 C alone showed the same association. Association between the kdr mutations and the susceptibility status of pyrethroids could not be established due to very small sample size. A low to moderate level of resistance was noticed against temephos among the larval population based on WHO criteria. Conclusion The replacement of DDT by type II pyrethroids for the management of dengue vectors is an appropriate decision taken by the national program which is supported by the findings of a higher level of resistance to DDT. Persistence of polymorphisms in the VGSC gene might be an indication of emergence of resistance against pyrethroid insecticides that should be monitored at a regular interval. Attempts should be made to determine the effectiveness of other larvicides for replacement of temephos if needed in future. Along with the chemical insecticides different biological vector control methods as well as biopesticides should also be used in vector control programmes.

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“…Since there are still very few specific anti‐viral treatments or safe vaccines that are suitable for Dengue fever, the most effective method of controlling or preventing dengue fever depends entirely on the control of and protection from the bites of the mosquito that transmits it. The primary strategies rely mainly on environmental management and chemical insecticides, including organophosphates such as temephos, fenthion and insect growth regulators targeted at larval or adult mosquitoes, such as diflubenzuron and methoprene, with effective, wide and repeated use of these synthetic compounds resulting in the appearance of resistant populations of Aedes mosquito . Additionally, non‐selective chemical usage has caused other serious problems, such as damage to natural ecosystems caused by non‐target organism killing .…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and larvicidal activity against Aedes mosquitoes of essential oils from Arisaema fargesii

Huang

Lin

Jia

et al. 2019

Pest Management Science

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BACKGROUND: Dengue fever is caused by the spread of dengue virus by Aedes mosquito vectors. Currently, the most effective way to control dengue is by preventing mosquitoes from spreading the disease. Arisaema fargesii is a Chinese herbal medicine commonly used to repel mosquitoes. In our laboratory, anti-mosquito chemical components were extracted from A. fargesii, and the effects of these substances on mosquito larvae were examined. RESULTS: In total, 48 compounds corresponding to 98.79% of the total oil were identified and the major compounds identified were linalool (12.38%), carvacrol (8.27%), eugenol (5.21%), and -selinene (5.36%). Essential oil had larvicidal activity against Ae. aegypti and Ae. albopictus with LC 50 values of 40.49 mg/L, 47.01 mg/L, respectively. The LC 50 values of carvacrol, eugenol, linalool and -selinene were 32.78, 56.34, 70.56, 136.03 mg/L against Ae. aegypti larvae, and 39.08, 52.07, 82.34, 151.74 mg/L, respectively, against Ae. albopictus larvae. Biochemical assays of Aedes larvae showed that the activities of acetylcholinesterase (AChE), monooxygenases (MO), glutathione-S-transferase (GST), p-Nitrophenyl acetate (p-NPA) esterase, -esterase and -esterase were significantly affected by carvacrol. Essential oil induced the detoxification mechanism for the action of GST and MO.CONCLUSION: The result indicates that essential oil of A. fargesii and its isolated constituent have good inhibitory effects on the defense enzymes of Aedes mosquito larvae. A. fargesii essential oil can be used to control Aedes mosquito larvae to prevent the spread of dengue fever. /journal/ps a Retention index relative to n-alkanes on HP-5 MS capillary column. b Relative area (peak area relative to the total peak area). c RI, retention index, MS, mass spectrum, Co, co-injection with authentic compound. Pest Manag Sci 2020; 76: 534-542

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Assessment of insecticide resistance in primary dengue vector, Aedes aegypti (Linn.) from Northern Districts of West Bengal, India

Cited by 20 publications

References 30 publications

Insecticide resistance mapping in the vector of lymphatic filariasis, Culex quinquefasciatus Say from northern region of West Bengal, India

Insecticide resistance mapping in the vector of lymphatic filariasis, Culex quinquefasciatus Say from northern region of West Bengal, India

Prevalence of kdr mutations and insecticide susceptibility among natural population of Aedes aegypti in West Bengal

Chemical composition and larvicidal activity against Aedes mosquitoes of essential oils from Arisaema fargesii

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