Assessment of oxidative damage and lipid profile levels in tobacco chewers with Type 2 diabetes mellitus

Background: Choriocarcinoma is a malignant trophoblastic tumor that can degrade the uterine basement membrane to facilitate local and distant metastasis. Matrix metalloproteinase-2 (MMP-2) is a vital proteinase produced by trophoblasts and can cleave type IV collagen in the basement membrane of the uterine epithelium. In addition, E-cadherin controls cell adhesion, which is associated with tumorprogression, and loss of E-cadherin function is associated with metastasis. Vitamin A analogues, including all-trans retinoic acid (ATRA), exhibit anticancer properties, including metastasis inhibition.This study aimed to investigate the ability of ATRA to prevent trophoblast invasion and metastasis in choriocarcinoma by assessing MMP-2 and E-cadherin activity. Methods: This study administered various doses of ATRA to the BeWo (American Type Culture Collection CCL-98) choriocarcinoma cell line and assessed MMP-2 and E-cadherin activity through flow cytometry. The research was performed at the Biomedical Laboratory of the Faculty of Medicine of Universitas Brawijaya Malang, East Java, Indonesia. Results: BeWo cells were exposed to 0, 50, 100, 200, 400, or 800 μg/mL ATRA. MMP-2 was downregulated after ATRA treatment. The greatest reduction in MMP-2 level was observed after 200 μg/mL ATRA treatment, but this result was not statistically significant (P = 0.550). However, ATRA treatment significantly reduced the number of BeWo cells with low E-cadherin levels (P = 0.012). Conclusion: ATRA downregulates MMP-2 and upregulates E-cadherin. Further in vitro and animal studies are required to evaluate the ability of ATRA to inhibit metastasis. Bangladesh Journal of Medical Science Vol. 22 No. 02 April’23 Page : 336-340

Section: Discussionmentioning

confidence: 99%

All-Trans Retinoic Acid Reduces Matrix Metalloproteinase-2 and Increases E-Cadherin Levels in BeWo Choriocarcinoma Cells

Nurseta

Harumsari

Prasetya

et al. 2023

“…Diabetes is a metabolic disease characterized by hyperglycemia and impaired carbohydrate metabolism, fat, and insulin resistance, and it becomes one of the causes of death in the world 4 . Hyperglycaemia convinced the characterize depressed oxidative aim to assist the development of organ failure and lead in to complication of DM 5 . Foot ulcers Diabetic (DFU) is the most serious complication of DM and the main cause of nontraumatic leg amputation 6 .…”

Section: Introductionmentioning

confidence: 99%

The Effect of Bitter Melon (Momordica charantia L.) Leaves Extractonon Glycated Albumin in Diabetic Foot Ulcers: Randomized Controlled Trial

Rosyid

Prasetyo

Prabawati

et al. 2021

Objective: This study aims to prove the effect of supplementation of bitter melon leaves extract on glycemic status in diabetic foot ulcer (DFU). Materials and Methods: This study used a randomized, double-blinded, and placebo-controlled trial. Thirty DFU patients based on PEDIS 1-8 scores who met the criteria were divided into 2 groups consisting of 15 patients as a treatment group with the administration of bitter melon leaves extract at a dose of 6 g/day and 15 patients as a control group of with placebo. This intervention was conducted in 4 weeks. Glycemic status was measured by the value of glycated albumin and examined at baseline and at the end of treatment. Data were analyzed using paired t-test and independent t-test. Results: After 4 weeks of treatment, there was an increase in the baseline value of glycated albumin in the treatment group from 24.5 ± 11.7% to 25.8 ± 12.7% or an increase of 1.3 ± 6.3% and a decrease in the control group from 27.5 ± 13.1% to 25.8 ± 8.5% or a decrease of 1.7 ± 12.4%. The results of the analysis of the effect of supplementation of bitter melon leaves extract on the value of glycated albumin were not significant (p = 0.99). Conclusion: The supplementation of bitter melon leaf extract with a dose of 6 g / day did not significantly affect the value of glycated albumin. Bangladesh Journal of Medical Science Vol.20(2) 2021 p.281-287

“…Coenzyme Q10 (CoQ10) is a carrier of electron and proton in mitochondrial respiratory chain and participates in cellular aerobic energy production. On the other hand, CoQ10 has some antioxidant property and acts as a non-enzymatic antioxidant that protects the body against damages by reactive oxygen species (ROS) and also restores other antioxidants 3,4 . It has been shown that CoQ10 synthesis is reduced in patients with type 2 diabetes mellitus (T2DM) which leads to increase ROS production and mitochondrial dysfunction.…”

Section: Introductionmentioning

confidence: 99%

Coenzyme Q10 supplementation effects on lipid ratios in women with type 2 diabetes mellitus: A randomized, double-blind clinical trial study

Zarei¹,

Rezvanfar²,

Khosrowbeygi³

2019

Objective: This study had been designed to evaluate the effects of Coenzyme Q10 (CoQ10) supplementation on the lipid ratios in women with type 2 diabetes mellitus (T2DM). Materials and Methods: Sixty-eight women with T2DM were enrolled and randomly divided into two groups. While one group received 100 mg/day of CoQ10 supplement for 12 weeks (n=34) the other was given placebo for the same time duration (n=34). Lipid profile including triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were evaluated at the beginning and at the end of the intervention and lipid ratios were calculated. Results and Discussion: After the intervention, values of non-HDL-C (p=0.001), TG/HDL-C (p=0.03) and LDL-C/HDL-C (p=0.001) were significantly decreased in CoQ10 group. Values of HDL ratio (p=0.002) were increased significantly in CoQ10 group. Conclusion: This study showed that daily supplementation with 100 mg of CoQ10 in women with T2DM could improve some atherogenic lipid ratios that might be useful in preventing cardiovascular diseases in them. Bangladesh Journal of Medical Science Vol.19(1) 2020 p.152-158