Assessment of the Antiangiogenic and Anti-Inflammatory Properties of a Maslinic Acid Derivative and its Potentiation using Zinc Chloride

Abstract

Maslinic acid is a pentacyclic triterpene with a plethora of biological activities, including anti-inflammatory, antioxidant, antimicrobial, cardioprotective, and antitumor effects. New derivatives with improved properties and broad-spectrum activity can be obtained following structural changes of the compound. The present study was aimed to characterize a benzylamide derivative of maslinic acid—benzyl (2α, 3β) 2,3-diacetoxy-olean−12-en-28-amide (EM2)—with respect to the anti-angiogenic and anti-inflammatory effects in two in vivo experimental models. Consequently, the compound showed good tolerability and lack of irritation in the chorioallantoic membrane assay with no impairment of the normal angiogenic process during the tested stages of development. In the acute ear inflammation murine model, application of EM2 induced a mild anti-inflammatory effect that was potentiated by the association with zinc chloride (ZnCl2). A decrease in dermal thickness of mice ears was observed when EM2 and ZnCl2 were applied separately or in combination. Moreover, hyalinization of the dermis appeared only when EM2 was associated with ZnCl2, strongly suggesting the role of their combination in wound healing.

<p>Thermosensitive Betulinic Acid-Loaded Magnetoliposomes: A Promising Antitumor Potential for Highly Aggressive Human Breast Adenocarcinoma Cells Under Hyperthermic Conditions</p>

“…The CAM assay is a simple alternative method to in vivo mouse models that offers the ability to assess the angiogenesis process. 47 The angiogenesis process is the phenomenon through which new blood vessels are emerging from the pre-developed vasculature. 48 This process is known to have a significant impact on tumor growth and proliferation.…”

“… 49 Thus, this method is widely used in cancer research as a preliminary assay to murine experimental models. 47 …”

Phytochemical and Biological Screening of Oenothera biennis L. Hydroalcoholic Extract

“…The experiment followed the rules and was approved by the Bioethical Committee of the Faculty of Pharmacy of Victor Babes University of Medicine and Pharmacy Timisoara, Romania (No. 1/13.01.2020) [44,45]. Five groups (n = 5), each consisting of 7 mice, were included in the study as follows: (i) control (surface~1 cm 2 -inner surface of the left ear); (ii) animals, to which the experimental animal model of inflammation was induced by using TPA dissolved in acetone (20 µL/mouse ear) (surface~1 cm 2 -inner surface of the right ear); (iii) animals, to which acetone (20 µL/mouse ear) was applied on the ear (surface~1 cm 2 -inner surface of the right ear); (iv) animals, to which indomethacin cream (4%) was typically applied as the treatment after the induction of the experimental animal model of inflammation (surface~1 cm 2 -inner surface of the right ear); (v) animals to which OB extract (300 µg/cm 2 ) was typically applied as the treatment after the induction of the experimental animal model of inflammation (surface~1 cm 2 -inner surface of the right ear) [46].…”

The Anticancer Potential of Maslinic Acid and Its Derivatives: A Review

“…[14][15][16][17] Maslinic acid [MA, (2α,3β)-2,3-dihydroxylolean-12-en-28-oic acid] (Figure 1) is a pentacyclic triterpene acid primarily derived from the olive tree (Olea europaea L.) and shanzha (Crataegus pinnatifida Bunge), with a C 30 H 48 O 4 molecular formula, molecular weight of 472.7, and melting point and boiling point of 267-269°C and 570.0 ± 50.0°C, respectively. [18][19][20] MA possesses a wide range of pharmacological benefits, including anti-cancer, 21 antiinflammatory and analgesic effects, [22][23][24] antiplatelet aggregation, 25,26 cardioprotective and antiinflammatory, 27,28 antimicrobial, 29,30 hepatoprotective, 31 anti-diabetic, and anti-hyperlipidemic, 32,33 to name a few. Among these effects, it has strong clinical potential.…”