Assessment of the floral origin of honey via proteomic tools

Girolamo, Francesco; D’Amato, Alfonsina; Righetti, Pier Giorgio

doi:10.1016/j.jprot.2012.04.029

Cited by 78 publications

(61 citation statements)

References 21 publications

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“…Honey also contains low amounts of protein, usually 0.1÷1.5% in the western honeybee A. mellifera , and 0.1÷3.0% in the Asiatic honeybee A. cerana . Most abundant peptides are defensin-1 and royal jelly protein (MRJP) isoforms, while major enzymes include glucose oxidase, diastase (amylase), α-glucosidase, catalase, and acid phosphatase (Kubota et al, 2004; Di Girolamo et al, 2012; Chua et al, 2015). Data about honey proteome are limited, but it is arguable that each honey type has its own peptide pattern, consisting of ubiquitous components and a variable set of minor elements, possibly including plant peptides.…”

Section: Honeymentioning

confidence: 99%

Therapeutic Properties of Bioactive Compounds from Different Honeybee Products

et al. 2017

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Honeybees produce honey, royal jelly, propolis, bee venom, bee pollen, and beeswax, which potentially benefit to humans due to the bioactives in them. Clinical standardization of these products is hindered by chemical variability depending on honeybee and botanical sources, but different molecules have been isolated and pharmacologically characterized. Major honey bioactives include phenolics, methylglyoxal, royal jelly proteins (MRJPs), and oligosaccharides. In royal jelly there are antimicrobial jelleins and royalisin peptides, MRJPs, and hydroxy-decenoic acid derivatives, notably 10-hydroxy-2-decenoic acid (10-HDA), with antimicrobial, anti-inflammatory, immunomodulatory, neuromodulatory, metabolic syndrome preventing, and anti-aging activities. Propolis contains caffeic acid phenethyl ester and artepillin C, specific of Brazilian propolis, with antiviral, immunomodulatory, anti-inflammatory and anticancer effects. Bee venom consists of toxic peptides like pain-inducing melittin, SK channel blocking apamin, and allergenic phospholipase A2. Bee pollen is vitaminic, contains antioxidant and anti-inflammatory plant phenolics, as well as antiatherosclerotic, antidiabetic, and hypoglycemic flavonoids, unsaturated fatty acids, and sterols. Beeswax is widely used in cosmetics and makeup. Given the importance of drug discovery from natural sources, this review is aimed at providing an exhaustive screening of the bioactive compounds detected in honeybee products and of their curative or adverse biological effects.

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Section: Honeymentioning

confidence: 99%

Therapeutic Properties of Bioactive Compounds from Different Honeybee Products

et al. 2017

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“…The most important authentic honeybee proteins in honey (5,25) are royal jelly (RJ) proteins designated as apalbumins, which belong to a protein family consisting of nine members with M r of 49-87 kDa. Apalbumin1 (apa1), apalbumin2 (apa2), and apalbumin3 (apa3) account for 90 % of the RJ protein content and have 72 % identical amino acid sequence (26)(27)(28)(29)(30).…”

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confidence: 99%

Major royal jelly proteins as markers of authenticity and quality of honey / Glavni proteini matične mliječi kao markeri izvornosti i kakvoće meda

Bíliková

Kraková

Yamaguchi³

et al. 2015

Archives of Industrial Hygiene and Toxicology

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Until now, the properties of honey have been defined based exclusively on the content of plant components in the nectar of given plant. We showed that apalbumin1, the major royal jelly (RJ) protein, is an authentic and regular component of honey. Apalbumin1 and other RJ proteins and peptides are responsible for the immunostimulatory properties and antibiotic activity of honey. For the quantification of apalbumin1, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal anti-apalbumin1 antibody. The method is suitable for honey authenticity determination; moreover it is useful for detection of the honey, honeybee pollen and RJ in products of medicine, pharmacy, cosmetics, and food industry, where presences of these honeybee products are declared. Results from the analysis for presence and amount of apalbumin1 in honeys will be used for high-throughput screening of honey samples over the world. On the basis of our experiments which show that royal jelly proteins are regular and physiologically active components of honey we propose to change the definition of honey (according to the EU Honey Directive 2001/110/EC) as follows: Honey is a natural sweet substance produced by honey bees from nectar of plants or from secretions of plants, or excretions of plant sucking insects, which honey bees collect, transform by combining with major royal jelly proteins and other specific substances of their own, deposit, dehydrate, store and leave in the honey comb to ripen and mature.

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“…El contenido de polen en las mieles es muy bajo (niveles trazas) pero varía dependiendo de la riqueza floral de cada zona y del aporte de néctar y polen de cada especie vegetal, por lo cual, algunas especies se encuentran sub-o sobre-representadas en el perfil palinológico y se reconoce la necesidad de establecer otras metodologías menos dependientes de las trazas de polen en la miel, como la proteómica (15) , la espectroscopia óptica y sensores electrónicos (16) o espectrometría de fluorescencia para estimar parámetros físico-químicos y contenido floral (17) , entre otros.…”

Section: Methodsunclassified

“…honey physicochemical parameters and botanical content, such as proteomics (15) , optical spectrophotometry and electronic sensors (16) , and fluorescence spectrophotometry (17) , among others.…”

Section: Introductionmentioning

confidence: 99%

Composición botánica de mieles de la península de Yucatán, mediante qPCR y análisis de curvas de disociación

Cázares¹,

Ordóñez

Cruz

et al. 2016

Rev. Mex. Cienc. Pecu.

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RESUMENEl método cuantitativo de reacción en cadena de la polimerasa (qPCR), seguido por análisis de curvas de disociación, fue desarrollado para la detección rápida y simultánea de la composición botánica en mieles de la Península de Yucatán, México. Cinco muestras de miel ciclo apícola 2013 y cinco del 2014, se caracterizaron para definir el contenido de cinco especies de plantas de importancia apícola; Viguiera dentata, Gymonopodium floribundum, Piscidia piscipula, Acacia angustissima y Mimosa bahamensis. These results correlate to melissopalynological analysis for most cases. P. piscipula was not detected in any honey sample; however, according to melissopalynological analysis A. angustissima was present in M-3 and M-4 even though it was unable to detect it, possibly due to a low or no similarity with generic genes sequence.

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Assessment of the floral origin of honey via proteomic tools

Cited by 78 publications

References 21 publications

Therapeutic Properties of Bioactive Compounds from Different Honeybee Products

Therapeutic Properties of Bioactive Compounds from Different Honeybee Products

Major royal jelly proteins as markers of authenticity and quality of honey / Glavni proteini matične mliječi kao markeri izvornosti i kakvoće meda

Composición botánica de mieles de la península de Yucatán, mediante qPCR y análisis de curvas de disociación

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