Association between single nucleotide polymorphism in ovine Calpain gene and growth performance in three Egyptian sheep breeds

Mahrous, Karima F.; Hassanane, M. S.; Shafey, Heba I.; Mordy, M. Abdel; Rushdi, Hossam E.

doi:10.1016/j.jgeb.2016.09.003

Cited by 23 publications

(17 citation statements)

References 22 publications

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“…Calpain substrates consist of a variety of enzymes such as cytoskeletal proteins (Fedota et al, 2016) and epidermal growth factor receptors (Glading et al, 2000). The CAPN gene was investigated as a potential candidate gene for growth trait as reported in earlier studies of Mahrous et al, 2016;Hou et al, 2011;and Bosques et al, 2015. Amplification of CAPN1 gene in 5'UTR fragment was successfully conducted using PCR for all samples.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Sihite

Priyanto

Jakaria

2019

Trop. Anim. Sci. J.

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The aim of this study was to identify the variation of 5'UTR CAPN1 gene and its association to growth traits in Bali cattle. DNA samples were obtained from 80 heads of Bali cattle originated from BPTU-HPT Denpasar. The average of Bali cattle age was 784 days (631 days-1098 days). Bali cattle were divided into 3 age groups namely, the first group (1.5 years to 2 years), the second group (2 years to 2.5 years), and the third group (2.5 years to 3 years). The observed growth traits were birth weight (kg), live weights (kg), average daily gain (kg), body length (cm), chest depth (cm), withers height (cm), hip height (cm), and heart girth (cm). Polymorphism identification of 5'UTR CAPN1 gene was conducted by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with BglII as the restriction enzyme. Growth traits data association were analyzed using the General Linear Model (GLM) analysis. The 5'UTR CAPN1 gene|BglII was polymorphic in Bali cattle (GG, GT, and TT). Genotype frequencies for Bali cattle were 0.30 (GG), 0.66 (GT), and 0.04 (TT). The allele frequencies of G and T allele were 0.63 and 0.37, respectively. The G allele was the most frequent allele and GT genotype was the most frequent genotype among the cattle. The CAPN1|BglII had a significant effect (p<0.05) on growth traits in Bali cattle. Animal carrier of GG genotype had higher live weight and average daily gain than those with GT genotype, while the lowest values were associated with TT genotype.

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Section: Discussionmentioning

confidence: 99%

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Sihite

Priyanto

Jakaria

2019

Trop. Anim. Sci. J.

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“…For instance, LIM Homeobox 3 (LHX3) and LIM Homeobox 4 (LHX4) serve as a transcription factors for sheep body growth and reproduction process [11,12]. A PCR study has identified genetic variants in ovine Calpain (CAPN) gene which are related with meat tenderness and growth performance traits in sheep [13][14][15]. Calpains (CAPN) encodes intracellular calcium-activated cysteine proteases that work in physiological and pathological processes.…”

Section: Genes Associated With Growth and Body Weightmentioning

confidence: 99%

Review on Genomic Regions and Candidate Genes Associated with Economically Important Production and Reproduction Traits in Sheep (Ovies aries)

Gebreselassie

Berihulay

Jiang

et al. 2019

Animals

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Simple Summary: Sheep is one of the most economically important animals used as a source of meat, milk, wool, and fur for human society. These commodities are essential for human being. Body growth, body weight, carcass quality, fat percent, fertility, milk yield, wool, horn type and coat color are essential and useful sheep traits. Understanding the genetic background of these traits is paramount to increase the production and productivity of domestic animals. The availability of genomic data, development of molecular breeding techniques, and genome technologies have come to play a vital role in understanding the genetic background of different animal traits. This is directly or indirectly helpful for the practice of genetic improvement of economically important traits in sheep. The identification of genomic regions, genes associated with phenotypic traits, and description of gene function are some of the applied research activities to understand the genetics of livestock species. The aim of this review is to discuss and summarize different reported research findings on identified genomic regions and candidate genes related with economically important traits as well as gene annotation in sheep.Abstract: Sheep (Ovis aries) is one of the most economically, culturally, and socially important domestic animals. They are reared primarily for meat, milk, wool, and fur production. Sheep were reared using natural selection for a long period of time to offer these traits. In fact, this production system has been slowing the productivity and production potential of the sheep. To improve production efficiency and productivity of this animal through genetic improvement technologies, understanding the genetic background of traits such as body growth, weight, carcass quality, fat percent, fertility, milk yield, wool quality, horn type, and coat color is essential. With the development and utilization of animal genotyping technologies and gene identification methods, many functional genes and genetic variants associated with economically important phenotypic traits have been identified and annotated. This is useful and presented an opportunity to increase the pace of animal genetic gain. Quantitative trait loci and genome wide association study have been playing an important role in identifying candidate genes and animal characterization. This review provides comprehensive information on the identified genomic regions and candidate genes associated with production and reproduction traits, and gene function in sheep.

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“…Egyptian breeds are fat-tailed that characterized by extended breeding seasons, high fertility, and well adapted to harsh environmental conditions (Galal et al, 2005;EL-Hanafy and El-Saadani, 2009Mahrous et al, 2016, Barakat et al, 2017El-Malky et al, 2019).The total number of Egyptian sheep breeds is raised from 5,463,169 head in 2015 to 5,697,716 head in 2017. Sheep provide 72,296 tons of meat, they contribute 6% of the total red meat produced in Egypt and 99,322 tons of fresh milk (FAO, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…It is a very useful technique in early prediction and comparing animals with different economically important characteristics like growth traits (Dunn and Ryan, 2015). The growth hormone (GH), insulin-like growth factor-1 (IGF-1) and leptin genes are candidates for growth in ovine since they play a key role in growth regulation and development (Barzehkar et al 2009, Nazari et al, 2016Mahrous et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Growth Performance, Blood Metabolites and Gene Expression Analysis in Egyptian Sheep Breeds, in Relation to Age

Ashour¹,

Gad²,

Fayed³

et al. 2020

jwpr

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The growth performance of lambs attributes the economic viability of animals. Faster growth allows lambs to reach maturity in early age. Therefore, the aim of this study was to compare growth performance, blood metabolites and expression of IGF-1, GH, and Leptin genes in three different Egyptian sheep breeds across age. Thirty Egyptian sheep males from three breeds (Ossimi, Rahmani and Barki) were divided into three ages categorize (7-9, 10-12, and 13-16 months). The results showed that there was a significant increase in sheep's live body weights toward advanced ages till the second age category for all breeds, the highest values of linear body measurements were observed in Ossimi breed. There was a non-significant inverse effect of advanced age on blood glucose and total lipids levels in all sheep breeds. There wasn't any significant effect of interaction between age and breed on plasma total protein concentrations. According to age categories, Barki breed showed a significant up-regulation of GH compared to the Ossimi breed in 7-9 months age category. However, Barki breed showed a significant downregulation of IGF-1compared to the Ossimi breed in 7-9 months. Meanwhile, Leptin expression showed significant differences in Ossimi breed between 10-12 months age category and two other age categories. We concluded that measuring of physical body measurements, blood metabolites and GH, IGF-1 and Leptin genes in early ages is a good and accurate indicator for growth performance in Egyptian sheep breeds.

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Association between single nucleotide polymorphism in ovine Calpain gene and growth performance in three Egyptian sheep breeds

Cited by 23 publications

References 22 publications

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Review on Genomic Regions and Candidate Genes Associated with Economically Important Production and Reproduction Traits in Sheep (Ovies aries)

Evaluation of Growth Performance, Blood Metabolites and Gene Expression Analysis in Egyptian Sheep Breeds, in Relation to Age

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