Astrocyte and microglial motility in vitro is functionally dependent on the hyaluronan receptor RHAMM

Turley, Eva A.; Hossain, Mohammad Zakir; Sorokan, T; Jordan, Larry M.; Nagy, J. I.

doi:10.1002/glia.440120109

Cited by 50 publications

(34 citation statements)

References 71 publications

(97 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the immunolocalization data provide strong evidence that links the initial 12 h of increased motility following injury with the redistribution of RHAMM to the cell surface followed by its disappearance so that, by 18 h, only nuclear staining was observed. We have previously correlated rate of locomotion with cell surface localization of RHAMM in other cell types (27,43,44). The significance of the nuclear staining is unknown, but has previously been noted in fibroblasts (27).…”

Section: Discussionmentioning

confidence: 89%

Migration of bovine aortic smooth muscle cells after wounding injury. The role of hyaluronan and RHAMM.

Savani¹,

Wang²,

Yang³

et al. 1995

J. Clin. Invest.

191

161

View full text Add to dashboard Cite

The migration of smooth muscle cells is a critical event in the pathogenesis of vascular diseases. We have investigated the role of hyaluronan (HA) and the hyaluronan receptor RHAMM in the migration of adult bovine aortic smooth muscle cells (BASMC). Cultured BASMC migrated from the leading edge of a single scratch wound with increased velocity between 1 and 24 h. Polyclonal anti-RHAMM antisera that block HA binding with this receptor abolished smooth muscle cell migration following injury. HA stimulated the random locomotion of BASMC and its association with the cell monolayer increased following wounding injury. Immunoblot analysis of wounded monolayers demonstrated a novel RHAMM protein isoform that appeared within one hour after injury. At the time of increased cell motility after wounding, FACS® analysis demonstrated an increase in the membrane localization in -25% of the cell population. Confocal microscopy of injured monolayers confirmed that membrane expression of this receptor was limited to cells at the wound edge. Collectively, these data demonstrate that RHAMM is necessary for the migration of smooth muscle cells and that expression and distribution of this receptor is tightly regulated following wounding of BASMC monolayers. (J. Clin. Invest. 1995Invest. . 95:1158Invest. -1168

show abstract

Section: Discussionmentioning

confidence: 89%

Migration of bovine aortic smooth muscle cells after wounding injury. The role of hyaluronan and RHAMM.

Savani¹,

Wang²,

Yang³

et al. 1995

J. Clin. Invest.

191

161

View full text Add to dashboard Cite

show abstract

“…CD168, a RHAMM, has a dual function as an extracellular and an intracellular hyaluronan-binding-protein (25,26). Hyaluronan serves an important role in tissue organization and in cellular migration, growth and differentiation (27).…”

Section: Discussionmentioning

confidence: 99%

Expression patterns of CD168 correlate with the stage and grade of squamous cell carcinoma of head and neck

Jöhrens

Anagnostopoulos

Dommerich

et al. 2017

Molecular and Clinical Oncology

View full text Add to dashboard Cite

Abstract. The receptor for hyaluronan-mediated motility CD168 is associated with the processes of oncogenesis and metastasis. The objective of the present study was to determine the possible association between the expression and distribution of CD168 and the tumor stage of head-and-neck squamous cell carcinoma (SCC). Formalin-fixed and paraffinembedded tumor samples obtained from 100 patients during primary resection of SCC from the oral cavity, oropharynx, hypopharynx or larynx were included in the present study. The patients were divided into two risk groups: Low risk, representing the early stage of completely resected SCCs with good-to-moderate differentiation, and the high-risk group, representing the advanced stage SCCs with positive resection margins, vascular invasion or locoregional metastasis. All specimens were stained with a monoclonal antibody against CD168. Percentage and staining intensity of CD168-positive cells were scored, and their spatial distribution within the tumor nests was noted. The results obtained were correlated with the tumor stage. The quantification of CD168 expression revealed significant differences between the two risk groups (t-test, P=0.002), with higher scores in tumors resected from the highrisk SSC group compared with those from the low-risk group. In addition, in the high-risk group, the CD168-positive cells were present predominantly in the periphery (70.4%) of tumor nests, whereas in the low-risk group, only 56.6% were located there; however, this trend did not reach the level of statistical significance. Taken together, the results from the present study suggested that CD168 expression patterns could potentially be used as a predictor of tumor aggressiveness, and therefore they may be a prognostic factor in head-and-neck SCC.

show abstract

“…Multiple studies over the last several decades provide a connection between cell motility and HA [73][74][75][76][77]. One such study, comparing metastases and primary tumor staining, found that RHAMM expression was significantly higher in the metastatic cells.…”

Section: Receptor For Hyaluronic Acid Mediated Motilitymentioning

confidence: 99%

Functional Aspects of the Hyaluronan and Chondroitin Sulfate Receptors

Harris¹,

Weigel²

2008

Animal Lectins

View full text Add to dashboard Cite

Astrocyte and microglial motility in vitro is functionally dependent on the hyaluronan receptor RHAMM

Cited by 50 publications

References 71 publications

Migration of bovine aortic smooth muscle cells after wounding injury. The role of hyaluronan and RHAMM.

Migration of bovine aortic smooth muscle cells after wounding injury. The role of hyaluronan and RHAMM.

Expression patterns of CD168 correlate with the stage and grade of squamous cell carcinoma of head and neck

Functional Aspects of the Hyaluronan and Chondroitin Sulfate Receptors

Contact Info

Product

Resources

About