Atomic force microscopy and confocal laser scanning microscopy on the cytoskeleton of permeabilised and embedded cells

Meller, Karl; Theiß, Carsten

doi:10.1016/j.ultramic.2005.10.003

Cited by 28 publications

(19 citation statements)

References 27 publications

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“…6). F-actin geodesic configurations have been observed in multiple cell lines using fluorescent microscopy and more recently in permeabilized and fixed cells using contact mode AFM under dry conditions (Lazarides and Weber, 1974;Lazarides, 1976;Osborne et al, 1978;Heuser and Kirschner, 1980;Miller and Theiss, 2005). Referring to geodesic structures Fuller (1965) stated that tension dependent structures gain their stability by "triangulating" their internal supporting elements.…”

Section: Resultsmentioning

confidence: 99%

Direct mechanical measurement of geodesic structures in rat mesenchymal stem cells

et al. 2007

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Section: Resultsmentioning

confidence: 99%

Direct mechanical measurement of geodesic structures in rat mesenchymal stem cells

et al. 2007

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“…Combined optical microscope and high-speed AFM [33][34][35][36] or conventional Raman scattering [37,38] microscopes, coherent anti-Stokes Raman scattering [39,40] microscopes, or TIRFM [41][42][43]. When still images are taken, simultaneous recording of optical and AFM images is not necessarily required.…”

Section: Visibilitymentioning

confidence: 99%

High-speed AFM and nano-visualization of biomolecular processes

Ando

Uchihashi

Kodera

et al. 2007

Pflugers Arch - Eur J Physiol

238

178

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Conventional atomic force microscopes (AFMs) take at least 30-60 s to capture an image, while dynamic biomolecular processes occur on a millisecond timescale or less. To narrow this large difference in timescale, various studies have been carried out in the past decade. These efforts have led to a maximum imaging rate of 30-60 ms/ frame for a scan range of~250 nm, with a weak tip-sample interaction force being maintained. Recent imaging studies using high-speed AFM with this capacity have shown that this new microscope can provide straightforward and prompt answers to how and what structural changes progress while individual biomolecules are at work. This article first compares high-speed AFM with its competitor (single-molecule fluorescence microscopy) on various aspects and then describes high-speed AFM instrumentation and imaging studies on biomolecular processes. The article concludes by discussing the future prospects of this cutting-edge microscopy.

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“…Much work has been done to overcome the limits of topological analysis and to investigate intracellular structures by AFM imaging (Melling et al, 2003;Franz and Muller, 2005;Meller and Theiss, 2006). To remove the plasma membrane of cells, two approaches, de-roofing by short ultrasonic burst and detergent extraction, have been demonstrated (Heuser, 2000;Berdyyeva et al, 2005).…”

Section: De-roofing and Detergent Extraction Of The Plasma Membranementioning

confidence: 99%

“…Several reports describe investigations of intracellular cytoskeletal structures after detergent extraction using the nonionic detergent Triton X-100 (Berdyyeva et al, 2005;Meller and Theiss, 2006). A method of permeabilization and embedding of lens epithelial cells was used to study the organization and distribution of intracellular proteins with AFM and confocal microscopy (Meller and Theiss, 2006).…”

Section: De-roofing and Detergent Extraction Of The Plasma Membranementioning

confidence: 99%

“…A method of permeabilization and embedding of lens epithelial cells was used to study the organization and distribution of intracellular proteins with AFM and confocal microscopy (Meller and Theiss, 2006). In this study, confocal microscopy was used to identify actin microfilaments by fluorescent labeling with rhodamine phalloidin, whereas AFM enabled the study of three-dimensional structures of microfilaments at the molecular level in fixed and living cells.…”

Section: De-roofing and Detergent Extraction Of The Plasma Membranementioning

confidence: 99%

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Molecular imaging of membrane proteins and microfilaments using atomic force microscopy

Jung

Park

et al. 2010

Exp Mol Med

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Atomic force microscopy (AFM) is an emerging technique for a variety of uses involving the analysis of cells. AFM is widely applied to obtain information about both cellular structural and subcellular events. In particular, a variety of investigations into membrane proteins and microfilaments were performed with AFM. Here, we introduce applications of AFM to molecular imaging of membrane proteins, and various approaches for observation and identification of intracellular microfilaments at the molecular level. These approaches can contribute to many applications of AFM in cell imaging.

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Atomic force microscopy and confocal laser scanning microscopy on the cytoskeleton of permeabilised and embedded cells

Cited by 28 publications

References 27 publications

Direct mechanical measurement of geodesic structures in rat mesenchymal stem cells

Direct mechanical measurement of geodesic structures in rat mesenchymal stem cells

High-speed AFM and nano-visualization of biomolecular processes

Molecular imaging of membrane proteins and microfilaments using atomic force microscopy

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