Augmentation of catecholamine release elicited by an Eugenia punicifolia extract in chromaffin cells

Pascual, Ricardo de; Colmena, Inés; Rı́os, Cristóbal de los; Rosa, Juliana M.; Leite, Paulo Emílio Corrêa; Lima-Araújo, Katia G.; Ferreira, Vı́tor F.; Rocha, David R. da; Gonzaga, Daniel T. G.; Garcı́a, Antonio G.; Santos, Wilson C.; Gandı́a, Luis

doi:10.1590/s0102-695x2011005000191

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…For instances, aqueous extract from E. punicifolia leaves hydrodistillation enhanced cholinergic nicotinic neurotransmission in rat diaphragm (Grangeiro et al 2006), but increased K ? -elicited secretion without enhancing the whole-cell inward calcium current in adrenal chromaffin cells (de Pascual et al 2012), and further exerted a potent antiinflammatory activity in skeletal muscles of dystrophic mutant mdx mice through a yet unknown mechanism.…”

Section: Introductionmentioning

confidence: 99%

Implant of Polymer Containing Pentacyclic Triterpenes from Eugenia punicifolia Inhibits Inflammation and Activates Skeletal Muscle Remodeling

Leite

Lima-Araújo

França

et al. 2014

Arch. Immunol. Ther. Exp.

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Sustained chronic inflammation induces activation of genes involved in cellular proliferation and apoptosis, thereby causing skeletal muscle degeneration. To investigate in vitro effects of isolated pentacyclic triterpenes from Eugenia punicifolia (Ep-CM) upon signaling pathways involved in the regulation of skeletal muscle cell line proliferation, and in vivo muscular tissue remodeling. C2C12 cells were seeded on eight-well plates and [(3)H]-thymidine incorporation, TUNEL assays, mitochondria viability, zymography for matrix metalloproteases (MMPs), Western blot analysis for MAPKinase signaling pathway, NFκB activation and HMGB1 production subsequently determined under basal conditions and after Ep-CM treatment. A polymer containing Ep-CM was implanted on the volar surface of gastrocnemius muscles subjected to acute injury induced by bupivacaine for local slow and gradual release of bioactive compounds, and mice killed 4 days after surgery. Ep-CM inhibited proliferation of C2C12 myoblast cell line in a dose-dependent manner, confirmed by reduction of [(3)H]-thymidine uptake without affecting cell viability or inducing apoptosis. The cytostatic effect of Ep-CM occurred mainly via inhibition of phosphorylated extracellular signal-regulated kinase (pERK) activation and DNA synthesis, possibly inhibiting the G1 phase of the cell cycle, since Ep-CM increased pAkt and p27(kip1) but reduced Cyclin D1. Ep-CM in vitro treatment increased MMP-9 and MMP-2 activities of C2C12 myoblast cells, but reduced in vivo MMP-9 activity and acute muscular inflammation. Besides cytostatic and anti-inflammatory effects, Ep-CM pentacyclic triterpenes also contributed to degradation of basement membrane components by activating mechanisms of skeletal muscle remodeling in response to local injury.

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Section: Introductionmentioning

confidence: 99%

Implant of Polymer Containing Pentacyclic Triterpenes from Eugenia punicifolia Inhibits Inflammation and Activates Skeletal Muscle Remodeling

Leite

Lima-Araújo

França

et al. 2014

Arch. Immunol. Ther. Exp.

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“…1 It's leaves are popularly used in the form of decoction or aqueous infusion to treat inflammation, like fever, flu, diabetes; in alcoholic infusions for the treatment of wounds and infectious diseases. [2][3][4] Scientific studies reported in the literature describe hypoglycemic, 5,6 antioxidant, [7][8][9] anti-inflammatory, antinociceptives 3,4,10 neuroprotective, 11 gastroprotective, 4,12 vasodilatory 13 and antiproliferative 14,15 activities of E. punicifolia leaves.…”

Section: Introductionmentioning

confidence: 99%

Validation HPLC-DAD Method for Quantification of Gallic and Ellagic Acid from Eugenia punicifolia Leaves, Extracts and Fractions

Silva¹,

Oliveira²,

Martins³

et al. 2023

J. Braz. Chem. Soc.

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Eugenia punicifolia (Kunth) DC, known as “pedra ume-caá” or “pitanguinha do cerrado”, is popularly used to treat infectious diseases like inflammation, and diabetes. Therefore, this study aimed to develop and validate an analytical method by high-performance liquid chromatography with diode array detector (HPLC-DAD) for the seasonal quantification of gallic acid (GA) and ellagic acid (EA), and to co-validate the method for quantification from the ethanol extract and fractions of the E. punicifolia leaves. Chromatographic separations were performed with a mobile phase gradient of acetonitrile/methanol/water acidified with 0.2% formic acid, a flow of 0.8 mL min‑1, detection at 254 nm, and a C18 column (250 × 4.6 mm, 5 μm). Method validation parameters were determined following Brazilian legislation. The HPLC-DAD analytical method developed for the quantification of GA and EA was validated and demonstrated to be selective, linear, precise, accurate, robust, and without matrix effect. Accordingly, it is helpful for the analysis of these tannins in crude ethanol extracts and fractions from E. punicifoila leaves. Thus, our results may be of great help for quality control assessment and standardization of raw materials containing Eugenia punicifolia.

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“…The leaves are popularly used in the form of a decoction or aqueous infusion to treat inflammation, fever, flu, diabetes, in alcoholic infusions for the treatment of wounds and infectious diseases, diarrhea, stomach disorders, and as a hypoglycemic (Oliveira, et al, 2005;Milk, et al, 2010;Basting, et al, 2014, Pascual, et al, 2012.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of the chemical composition, larvicidal, antimicrobial and cytotoxic activities of volatile oils from E. punicifolia leaves from Minas Gerais and Goiás

Silva

Martins

Abrão

et al. 2021

RSD

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Eugenia punicifolia (Kunth) D.C., Myrtaceae, known as “pedra-ume-caá”, is popularly used in the treatment of inflammation, infections, fever, flu, diabetes, and diarrhea. This study aimed to carry out a comparative study of the chemical composition of volatile oil from E. punicifolia leaves collected in Goiás and Minas Gerais, as well as to evaluate the larvicidal activity against Aedes aegypti L3 larvae, the antimicrobial activity against bacteria, pathogenic fungi, and environmental, and cytotoxic activity to Balb 3T3 cells (murine fibroblasts). Volatile oils were obtained by hydrodistillation in a Clevenger apparatus and analyzed by Gas Chromatography Coupled to Mass Spectrometry (CG/MS). A total of 60 compounds were identified, the main components found in the leaves of Goiás being Germacrene D, bicyclogermacrene and β-longipenene and in the leaves collected in Minas Gerais they were (Z)-caryophyllene, γ-cadinene, spathulenol, caryophyllene oxide, and α-cadinol. The larvicidal effect was moderate against Ae. aegypti, with LC50 of 85.53 µg / mL for samples from Goiás and LC50 of 91.52 µg / mL for samples from Minas Gerais. Both oils showed moderate bactericidal activity against K. rhiziphyla (ATCC 9341), M. luteus (ATCC 10240), and S. aureus (ATCC 29737). The oils from Goiás (IC50 706.7 µg / mL) and Minas Gerais (IC50 160.7 µg / mL) had a lower cytotoxic concentration than the toxic action for larvae and bacteria, evidencing a safety profile and an interesting therapeutic potential, mainly concerning to volatile oil from Goiás. Therefore, the volatile oils from E. punicifolia leaves collected in Goiás and Minas Gerais that presented moderate larvicidal activity for Ae. aegypti also presented a bactericide activity and less cytotoxicity against murine fibroblasts. This is the first study of the larvicidal, antimicrobial and cytotoxic activity of volatile oils from E. punicifolia leaves.

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Augmentation of catecholamine release elicited by an Eugenia punicifolia extract in chromaffin cells

Cited by 5 publications

References 38 publications

Implant of Polymer Containing Pentacyclic Triterpenes from Eugenia punicifolia Inhibits Inflammation and Activates Skeletal Muscle Remodeling

Implant of Polymer Containing Pentacyclic Triterpenes from Eugenia punicifolia Inhibits Inflammation and Activates Skeletal Muscle Remodeling

Validation HPLC-DAD Method for Quantification of Gallic and Ellagic Acid from Eugenia punicifolia Leaves, Extracts and Fractions

Comparative study of the chemical composition, larvicidal, antimicrobial and cytotoxic activities of volatile oils from E. punicifolia leaves from Minas Gerais and Goiás

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