Avaliação preliminar da atividade antimicrobiana do extrato de erva-mate (Ilex paraguariensis A. St. - Hil.) obtido por extração com CO2 supercrítico

Abstract

") is a tree of the Aquifoliaceae family, native to the south region of South America and of great cultural and economic importance for the three states of the south region of Brazil. The present study aimed to determine the antimicrobial potential of "erva mate" extract obtained by supercritical fluid against some microorganism strains. The leaves of Ilex paraguariensis were collected in the north region of Rio Grande do Sul State. The crude extract of dehydrated plant was obtained by supercritical fluid extraction, using carbon dioxide (CO 2 ) as solvent. The extract mother solution (100 mg mL -1 ) was diluted (50; 25; 12, 5; 6.25 mg mL -1 ) for antimicrobial activity evaluation, by means of disk diffusion method on Müeller-Hinton agar, against strains of Acinetobacter baumannii (ATCC 19606), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 29212). The analyses were done in triplicate, using as negative control sterile disk without impregnation and as positive control, commercial antibiotic disks according to the bacterial species. After 16-24h incubation at 35ºC, the diameters of inhibition zones were measured. Antimicrobial activity was observed against S. aureus (inhibition zones between 7 and 18 mm) and P. aeruginosa (inhibition zones between 6 and 11 mm), both at the concentrations of 100 mg mL -1 to 25 mg mL -1 . The extract of Ilex paraguariensis obtained by supercritical fluid presented antimicrobial activity against S. aureus and P. aeruginosa.

Phytochemical profile, antioxidant and antimicrobial activity of extracts obtained from erva-mate (Ilex paraguariensis) fruit using compressed propane and supercritical CO2

“…However, literature is somewhat scarce on the vegetable matrices extraction using pressurized propane (Illés et al 1997(Illés et al , 2000Hamdan et al 2008;Daood et al 2002;Corso et al 2010;Freitas et al 2008;Silva et al 2015a, b;Santos et al 2015;Zanqui et al 2015a, b;Jesus et al 2013;Pederssetti et al 2011;Nimet et al 2001;Sparks et al 2006). Carelli et al (2011) extracted Ilex paraguariensis leaves using supercritical CO 2 . The extract obtained demonstrated potential for natural antibiotics from this plant due to the antimicrobial activity exhibited against microorganisms such as Staphylococcus aureus and Pseudomonas aeruginosa.…”

Chewing gums with yerba mate and different flavors: An initial study with consumers

“…Because of this yerba mate has been largely used as a stimulant. Studies suggested that yerba mate leaves are responsible for many other pharmacological antioxidant‐like, antidiabetic, diuretic, anti‐inflammatory, hepatoprotective, and antimicrobial properties (Alkhatib & Atcheson, 2017; Carelli et al., 2011; Yuan & Yuk, 2018).…”

Antioxidant action in diets with ground soybeans on ruminal microbial production, digestion, and fermentation in buffaloes

“…Ilex paraguariensis A. St. Hill, commonly known as yerba mate (YM) is a tree belonging to the family Aquifoleaceae. It is native to South America and is of great cultural and economic importance for southern Brazil (Carelli et al, 2011), where it is one of the main agricultural products. Although YM is used currently only for the preparation of beverages such as mate, tea, and soft drinks, it has great potential for alternative uses due to the diversity of its phytochemical composition.…”