Bacillus cereus phospholipases, enterotoxins, and other hemolysins

Lindbäck, Toril; Granum, Per Einar

doi:10.1016/b978-0-12-800188-2.00029-x

Cited by 7 publications

(7 citation statements)

References 130 publications

(164 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In eukaryotes, phosphoinositide-specific phospholipase C (PI-PLC) is an omnipresent protein that is involved in the phosphatidylinositol signaling system. It cleaves phosphatidylinositol triphosphate to form diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP3), which are primary signaling molecules to activate protein kinase C (PKC) and mobilize intracellular Ca 2+ , respectively [ 55 ]. Additionally, Ca 2+ acts as an important messenger to regulate metabolic pathways in many plants under stress [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

Yan

Mao

2021

IJMS

View full text Add to dashboard Cite

Melatonin priming is an effective strategy to improve the germination of aged oat (Avena sativa L.) seeds, but the mechanism involved in its time-course responses has remained largely unknown. In the present study, the phenotypic differences, ultrastructural changes, physiological characteristics, and proteomic profiles were examined in aged and melatonin-primed seed (with 10 μM melatonin treatment for 12, 24, and 36 h). Thus, 36 h priming (T36) had a better remediation effect on aged seeds, reflecting in the improved germinability and seedlings, relatively intact cell ultrastructures, and enhanced antioxidant capacity. Proteomic analysis revealed 201 differentially abundant proteins between aged and T36 seeds, of which 96 were up-accumulated. In melatonin-primed seeds, the restoration of membrane integrity by improved antioxidant capacity, which was affected by the stimulation of jasmonic acid synthesis via up-accumulation of 12-oxo-phytodienoic acid reductase, might be a candidate mechanism. Moreover, the relatively intact ultrastructures enabled amino acid metabolism and phenylpropanoid biosynthesis, which were closely associated with energy generation through intermediates of pyruvate, phosphoenolpyruvate, fumarate, and α-ketoglutarate, thus providing energy, active amino acids, and secondary metabolites necessary for germination improvement of aged seeds. These findings clarify the time-course related pathways associated with melatonin priming on promoting the germination of aged oat seeds.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

Yan

Mao

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The abolished pathogenicity via NCI was accompanied by malfunction of 37 genes encoding a variety of cuticle-degrading enzymes (Table S7). Markedly repressed genes included two adhesin-coding genes (BBA_02800 and BBA_06599) putatively involved in fungal attachment to insect cuticle and a novel hemolysin III family gene (MSTRG.10594) associated with microbial virulence [60]. Also listed in Table S7, dozens of dysregulated genes correlated with reduced tolerance to stress cues, such as those involved in responses to oxidative, cell wall perturbing, thermal and UV irradiating stresses.…”

Section: Transcriptomic Insight Into Profound Effect Of Dim5 On Fungal Lifecyclementioning

confidence: 99%

DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36

et al. 2021

View full text Add to dashboard Cite

Mono-, di-and tri-methylation of histone H3 Lys 9, Lys 4, and Lys 36 (H3K_me1/me2/me3) required for mediation of DNA-based cellular events in eukaryotes usually rely upon the activities of histone lysine methyltransferases (KMTs) classified to the KMT1, KMT2, and KMT3 families, respectively. Here, an H3K9-specific DIM5/KMT1 orthologue, which lacks a C-terminal post-SET domain and localizes mainly in nucleus, is reported to have both conserved and noncanonical roles in methylating the H3 core lysines in Beauveria bassiana, an insect-pathogenic fungus serving as a main source of wide-spectrum fungal insecticides. Disruption of dim5 led to abolishment of H3K9me3 and marked attenuation of H3K4me1/me2, H3K9me1/me2 and H3K36me2. Consequently, the Δdim5 mutant lost the whole insect pathogenicity through normal cuticle infection, and was compromised severely in virulence through cuticle-bypassing infection (hemocoel injection) and also in a series of cellular events critical for the fungal virulence and lifecycle in vivo and in vitro, including reduced hyphal growth, blocked conidiation, impeded proliferation in vivo, altered carbohydrate epitopes, disturbed cell cycle, reduced biosynthesis and secretion of cuticle-degrading enzymes, and increased sensitivities to various stresses. Among 1,201 dysregulated genes (up/down ratio: 712:489) associated with those phenotypic changes, 92 (up/down ratio: 59:33) encode transcription factors and proteins or enzymes involved in posttranslational modifications, implying that the DIM5-methylated H3 core lysines could act as preferential marks of those transcription-active genes crucial for global gene regulation. These findings uncover a novel scenario of DIM5 and its indispensability for insect-pathogenic lifestyle and genome stability of B. bassiana.

show abstract

“…In the study by Bach et al [40] the isolate Bacillus pumilus 7 PB also showed genes indicating virulence factors, such as hlyIII, capABCD. Hemolysin III (hly III) acts by forming oligomeric pores in three steps, rst, the protein binds to the erythrocyte surface and the monomers are then assembled to form the transmembrane pore, leading to erythrocyte lysis [41]. Although hemolytic activity poses a concern in probiotic safety, different Bacillus strains isolated from commercially available probiotics have been identi ed as hemolytic [42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Bacillus altitudinis 1.4 genome analysis - functional annotation of probiotic properties and immunomodulatory activity

Jankoski

Bach

Fonseca

et al. 2023

Preprint

View full text Add to dashboard Cite

Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. They are often used to improve intestinal health. In this study, phenotypic and genotypic methods were used to evaluate the probiotic properties of Bacillus altitudinis 1.4. The isolate was sensitive to all tested antimicrobials and showed a positive result in the hemolysis test. The results also indicated that the spores of B. altitudinis 1.4 were more resistant than the vegetative cells, when evaluated in a simulation of cell viability in the gastrointestinal tract, as well as adhesion to the intestinal mucosa. The isolate was able to self-aggregate and coaggregate with pathogens such as Escherichia coli ATCC 25922 and Salmonella Enteritidis ATCC 13076. Genomic analysis revealed the presence of genes with probiotic characteristics, such as fglA-L, motA, motB, luxS, pgaA-D, exoX, epsG. From this study, it was possible to evaluate the gene expression of pro-inflammatory and anti-inflammatory cytokines for different treatments. Viable vegetative cells of B. altitudinis 1.4 increased the transcription of pro-inflammatory factors, such as TNFα, NFkβ, TLR2, in addition to also increasing the transcription of IL-10, indicating a tendency to stimulate a pro-inflammatory profile. In view of the results presented, B. altitudinis 1.4 showed the potential to be applied in the incorporation of this microorganism in animal feed, since the spores could tolerate the handling and pelleting processes of the feed.

show abstract

Bacillus cereus phospholipases, enterotoxins, and other hemolysins

Cited by 7 publications

References 130 publications

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

Comparative Time-Course Physiological Responses and Proteomic Analysis of Melatonin Priming on Promoting Germination in Aged Oat (Avena sativa L.) Seeds

DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36

Bacillus altitudinis 1.4 genome analysis - functional annotation of probiotic properties and immunomodulatory activity

Contact Info

Product

Resources

About