Bacteriological and molecular detection of pseudomonas species from raw milk sold in Port-Said City markets

Mohammed, Gihan M.O.; Megahed, Amal A.; Nasr, Saad

doi:10.21608/ejceh.2015.253990

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…A total of 100 random samples of raw milk from different markets in Port-Said city were collected for isolation and identification of Pseudomonas species. Six isolates of Pseudomonas species were isolated from 100 raw milk samples and all the 6 (6%) isolates were identified as P. aeruginosa (Mohammed et al, 2015). This prevalence is lower than the prevalence in our study.…”

Section: Discussioncontrasting

confidence: 73%

Çiğ Süt Örneklerinden İzole Edilen Pseudomonas aeruginosa İzolatlarında Antibiyotik Direnci ve Biyofilm Özelliklerinin Belirlenmesi

Savaşan

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2022

Journal of Anatolian Environmental and Animal Sciences

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In this study, it was aimed to investigate the antibiotic resistance and biofilm properties of Pseudomonas aeruginosa isolates isolated from raw milks. A total number of 300 raw milk samples were collected from several dairy plants and vendors in the provinces of Aydın (n=100), İzmir (n=100) and Muğla (n=100) in Turkey. The conventional methods were used for the isolation of suspected Pseudomonas spp. from raw milk samples. A total of 63 suspected Pseudomonas spp. were isolated and these isolates were identified as being P.aeruginosa by PCR. According to PCR results, 24 isolates were identified as P.aeruginosa. The antibiotic resistances of P. aeruginosa against 8 antibiotics were determined by Kirby-Bauer Disc Diffusion method. The antibiotic resistance rates of the isolates among enrofloxacine, ceftriaxone, ciprofloxacin, meropenem, colistin, gentamycin, and azithromycin were found as 20.84%, 75.0%, 4.16%, 37.33%, 8.34%, 12.5 % and 91.66%, respectively. When examined according to multiple antibiotic resistances, it was determined that 11 isolates (45.83%) were resistant to more than 3 antibiotic groups and were evaluated as multi-resistant. The biofilm formations of the isolates were investigated in vitro with Congo Red Agar Method. The biofilm formation ratios were determined as 37.50% with CRA method. The biofilm formation and multiple antibiotic resistance rates were found as high in the raw milk isolates of P. aeruginosa. The widespread and advanced studies thought to be performed for the lightening of this situation and biofilm related antibiotic resistances.

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Section: Discussioncontrasting

confidence: 73%

Çiğ Süt Örneklerinden İzole Edilen Pseudomonas aeruginosa İzolatlarında Antibiyotik Direnci ve Biyofilm Özelliklerinin Belirlenmesi

Savaşan

Sezener

2022

Journal of Anatolian Environmental and Animal Sciences

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Association Between Biofilm Formation Gene Bla exoU and Metallo and Extend Spectrum Beta-lactamase Production of Multidrug Resistance Pseudomonas aeruginosa in Clinical Samples

Ali

2022

CCHTS

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Background: The presence of biofilm formation exoU gene is significant challenge to infection control management in hospitals and exposure by Pseudomonas aeruginosa may lead to further spread and development of antimicrobial resistance. Methods: Out of 227 samples 40 clinical isolates of P. aeruginosa were collected from patients attending public hospitals ( Rizgary, Teaching hospital, Laboratory center, Raparin, Nanakaly hospitals)in Erbil city/Iraq over a period during 2018 to march 2019 and fully characterized by standard bacteriological procedures and antimicrobial susceptibility test and ESBL has been carried out by Vitek 2 compact system and. by Vitek 2 compact system. The identification has been verified by all isolates as P. aeruginosa by using 16S rDNA with product size (956pb). Results: A high rate of resistance was seen against Penicillin and lincomycin and Piperacillin and chloramphenicol and rifampicin (100 %), whereas Imipenem (5%) were found to be the most effective antimicrobial drugs. Of all P. aeruginosa isolates, 30 (75% %) were identified as MDR, approximately 9(22.5%) of isolates were resistant to 9 drugs in burn samples. Quantitative biofilm determination using the Congo red method revealed that 28 isolates (70%) produced biofilm, biofilm production was significantly higher among MDR P. aeruginosa isolates while coproduction of Extended Spectrum β-lactamase (ESBL) together with Metallo β-lactamase (MBL) ESBLs MBLs recorded in (52.5%) of the isolates. Altogether 40 isolates were processed for analysis by PCR assays and showed that 26(70%) of P. aeruginosa isolates harboured the exoU encoding gene with product size (204) pb was more commonly seen in isolates obtained from burn isolates. In addition, exo U gene was significantly associated with the higher MDR (80%), 8 isolates (76.9%)had exoU gene with ESBL and( 65%) had MBL and the same for MDR (80.8%) in samples for burning. Conclusion: Our results showed surveillance of P. aeruginosa resistance against antimicrobial and ESBL and MBL is fundamental to monitor trends in susceptibility patterns and appropriately guide clinicians in choosing empirical or directed therapy.

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Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq

Ali,

Soor,

Ahmed

et al. 2024

Polish Journal of Microbiology

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Pseudomonas aeruginosa is a significant pathogen in hospital settings, notorious for its role in hospital-acquired infections and its ability to develop resistance to multiple antibiotics. This study investigates the prevalence, distribution, and antibiotic resistance gene profiles of P. aeruginosa in seven hospitals in Sulaymaniyah City. A total of 300 samples were collected from various hospital surfaces including mops, sinks, medical equipment, beds, desks, and floors. Using bacteriological, biochemical, and molecular methods, 66 isolates were confirmed as Pseudomonas species, with 26 identified as P. aeruginosa. Antibiotic susceptibility testing revealed resistance rates of 23.3% to streptomycin, 13.6% to tobramycin, 22.7% to moxifloxacin, 21.2% to levofloxacin, and 22.7% to norfloxacin. Furthermore, the antibiotic resistance gene detection showed the presence of the bla CTX-M, bla SHV, qnrB, and bla ACC-1 genes among the isolates. The study highlights a 22% contamination rate of hospital surfaces with Pseudomonas species, emphasizing the urgent need for enhanced infection control measures and targeted antimicrobial stewardship to manage and reduce the spread of multidrug-resistant P. aeruginosa.

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Bacteriological and molecular detection of pseudomonas species from raw milk sold in Port-Said City markets

Cited by 3 publications

References 13 publications

Çiğ Süt Örneklerinden İzole Edilen Pseudomonas aeruginosa İzolatlarında Antibiyotik Direnci ve Biyofilm Özelliklerinin Belirlenmesi

Çiğ Süt Örneklerinden İzole Edilen Pseudomonas aeruginosa İzolatlarında Antibiyotik Direnci ve Biyofilm Özelliklerinin Belirlenmesi

Association Between Biofilm Formation Gene Bla exoU and Metallo and Extend Spectrum Beta-lactamase Production of Multidrug Resistance Pseudomonas aeruginosa in Clinical Samples

Distribution and Molecular Characterization of Antibiotic-Resistant Pseudomonas aeruginosa in Hospital Settings of Sulaymaniyah, Iraq

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