A total of 100 random samples of raw milk were collected from different markets in Port-Said city aseptically for isolation and identification of Pseudomonas species. The results revealed that the Pseudomonas species could be detected in a percentage of 6% from raw milk samples. Six strains were identified biochemical as Pseudomonas aeruginosa. This study confirms the rapidity and sensitivity of 16SrDNA analysis in identifying strains which contribute in early monitoring, accurate analysis and control of microbial risks in food products. The described methodology has special relevance in raw milk quality control and safety. Molecular characterizations of P. aeruginosa were confirmed using 16SrDNA of Pseudomonas spp. and 16SrDNA of P. aeruginosa by conventional PCR. Which were developed with specific primers for the detection of different virulence genes (oprL, toxA, lasI, rhlR, ExoU, ExoS and ecfX) of P. aeruginosa, which may be considered a significant in food safety threat. P. aeruginosa possesses a variety of virulence genes that may contribute to its pathogenicity. Our results showed that oprL and oxA genes were detected in all 6 tested strains of P. aeruginosa, while lasI gene was detected in strains 1,2,3,4. But ExoS gene was detected only in 1, 2, and 3 strains. On the other hand Rhl1 gene was detected in 1, 3, and 4 tested strains. ExoU, rhlR, and ecfX genes not detect in any tested strains. PCR method is rapid than other diagnostic methods for the identification of P. aeruginosa strains, without using additional biochemical tests and It is a useful technique for detection its virulence genes. The public health hazard of this microorganism, as well as recommended measures to improve quality status of raw milk was discussed.

This study was carried out on slaughtered sheep and goats in different slaughter houses in Sharkia Province to determine the prevalence of ovine and caprine Sarcocystis infection and effect on some biochemical clinical pathological parameters. Parasitological examination of faecal samples revealed presence of eggs of internal parasites in 25(41.67%) lambs(less 9 month) {12(48%) nematodes, 7(28%) trematode) & 6(24%) mixed infection} 29 (48.33%) adult (more 2 year) (sheep {15(51.72%) nematodes,10 (34.48%) trematode & 4(13.79%) mixed infection},19 (47.50%) young goats {11(57.89%) nematodes, 5(26.32%) trematode & 3(15.79%) mixed infection}, 24 (60%) adult goats{14(58.33%) nematodes, 5(20.83%) trematode &5 (20.83 %) mixed infection}. Serologically, antibodies against Sporocystis spp. infection were observed in sheep serum samples were 47.50% (57) & 40% (32) for goats serum samples. The infection in adult animals were {34 (56.67%) in sheep and 19 (47.50%) in goats} more than in young animals {23 (38.33%) in sheep and13 (32.50 % in goats}. Sarcocystis in sheep distributed as macrocystis (16.67% for young and 11.67% adult), microcystis ovi-cans (10% young, 5% adult) and mixed infection (macrocystis and microcystis) were (56.67% young, 38.33% Prevalence of sarcocystis spp. In sheep and goats and its …

This patent describes the use of an ultrasonic imaging system to locate a calculus, the use of a needle system to deliver chemicals that dissolve the calculus, and the use of an ultrasonic therapeutic beam to insonify the calculus to enhance the dissolution of the calculus.mRCW 4,911,172 43.80.These transducers are comprised of a thin piezoelectric polymer film formed on a spiral ring and adhesively mounted on a support structure near the tip of a catheter. Electrical connection between the back face of the film and the support structure's negative electrode is via capacitive coupling. Connection to the front face of the film is made by a wire connected to the positive electrode of the catheter. A variation is formed by coating the tip of a catheter with a solution of PVDF.NRCW 4,913,159 43.80.Vj METHOD FOR DETERMINING BLOOD FLOW THROUGH A NARROWED ORIFICE USING COLOR DOPPLER ECHOCARDIOGRAPHY Julius M. Gardin et aL, assignors to Hitachi Medical Corporation and the University of California 3 April 1990 (Class 128/661.1); filed 17 March 1989 This method employs color Doppler images that contain two-color interfaces at locations where the flow velocity is equal to the aliasing velocity of the system. Distances from the two-color interfaces to the center of the orifice are used to calculate the volume flow rate through the orifice.N RCW 4,917,096 43.80.Vj PORTABLE ULTRASONIC PROBE Theodore M. Englehart, Richard F. Morris, and Narendra T. Sanghvi, assignors to Laboratory Equipment Corporation 17 April 1990 {Class 128/660.1}; filed 25 November 1987 This probe, which is intended for use with an ultrasonic imaging system, includes a fluid-filled enclosure 37 coupled to a handle 35 which houses a dc motor 61. The output shaft 64 is coupled to a level wind drive screw 75 which moves a transducer mounted in a holder assembly 78 back and forth. The travel of the transducer 80 creates a linear scan, thereby eliminating problems associated with sector scans in diagnostic imaging.mRCW 4,917,097 43.80.Vj APPARATUS AND METHOD FOR IMAGING