Hazmat is one of the personal protective equipment (PPE) used by healthcare professionals to combat infections such as Ebola, SARS, MERS, and the current prevalence of COVID-19. There are several things to consider when making this hazmat clothing. That is a type of hazmat material, standardization, and ease of use. Adding an outer layer of hazmat clothing containing betel leaf (Piper Betle. L) extract in an antibacterial and antiviral spray is expected to increase the safety of healthcare professionals and enhance protection from bacteria/viruses. In this course, we apply Research and Development (R&D) methods. The research steps were carried out experimentally in the laboratory by applying a betel leaf (Piper Betle. L) extract in a spray formulation. Spray formulation prepared at a leaf extract concentration; of 0%, 2.5%, 5%, and 7.5% and sprayed on a hazmat suit. Then the hazmat is used by health workers and tested for the number of bacteria in the laboratory. Based on the research, a spray with 7.5% leaf extract concentration showed maximum antibacterial activity in 16 bacterial colonies before the test and one colony after the test.