Basic fibroblast growth factor isoforms promote axonal elongation and branching of adult sensory neurons in vitro

Klimaschewski, Lars; Nindl, W; Feurle, Johannes; Kavakebi, Pujan; Kostron, Herwig

doi:10.1016/j.neuroscience.2004.04.004

Cited by 52 publications

(28 citation statements)

References 36 publications

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“…Channels containing Schwann cells overexpressing the high molecular weight isoform of FGF-2 are particularly useful for promoting regeneration, since axon sprouting appears to be reduced and recovery of thermoception is faster (Haastert, Lipokatic, Fischer, Timmer, & Grothe, 2006;Timmer, Robben, Muller-Ostermeyer, Nikkhah, & Grothe, 2003). This is consistent with our own observations that adult sensory neurons taken from prelesioned animals exhibit longer axons with a reduced number of branches if treated with FGF-2 versus NGF (Klimaschewski, Nindl, Feurle, Kavakebi, & Kostron, 2004). Due to their effects on mitogenesis of mesoderm-and neuroectoderm-derived cells, it is assumed that FGFs not only directly support axonal regeneration but also increase proliferation of Schwann cells and enhance angiogenesis (Aebischer et al, 1989).…”

Section: Functional Significance Of Neuronal Growth Factors As Putatisupporting

confidence: 88%

The Pros and Cons of Growth Factors and Cytokines in Peripheral Axon Regeneration

Klimaschewski

Hausott

Angelov

2013

International Review of Neurobiology

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Section: Functional Significance Of Neuronal Growth Factors As Putatisupporting

confidence: 88%

The Pros and Cons of Growth Factors and Cytokines in Peripheral Axon Regeneration

Klimaschewski

Hausott

Angelov

2013

International Review of Neurobiology

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“…FGF-2 influences the morphology and behavior of growth cones (Szebenyi et al, 2001) by local submembrane calcium signaling (Archer et al, 1999). Also, different FGF-2 isoforms have been shown to promote axonal elongation and branching of adult sensory neurons in vitro in response to a preconditioning lesion (Klimaschewski et al, 2004).…”

Section: Fgf-2 Promotes Nerve Regenerationmentioning

confidence: 99%

Faster nerve regeneration after sciatic nerve injury in mice over‐expressing basic fibroblast growth factor

Jungnickel¹,

Haase²,

Konitzer³

et al. 2006

J. Neurobiol.

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Basic fibroblast growth factor (FGF-2) is expressed in the peripheral nervous system and is up-regulated after nerve lesion. It has been demonstrated that administration of FGF-2 protects neurons from injury-induced cell death and promotes axonal regrowth. Using transgenic mice over-expressing FGF-2 (TgFGF-2), we addressed the importance of endogenously generated FGF-2 on sensory neuron loss and sciatic nerve regeneration. After sciatic nerve transection, wild-type and transgenic mice showed the same degree of cell death in L5 spinal ganglia. Also, the number of chromatolytic, eccentric, and pyknotic sensory neurons was not changed under elevated levels of FGF-2. Morphometric evaluation of intact nerves from TgFGF-2 mice revealed no difference in number and size of myelinated fibers compared to wild-type mice. One week after crush injury, the number of regenerated axons was doubled and the myelin thickness was significantly smaller in transgenic mice. After 2 and 4 weeks, morphometric analysis and functional tests revealed no differences in recovery of sensory and motor nerve fibers. To study the role of FGF-2 over-expression on Schwann cell proliferation during the early regeneration process, we used BrdU-labeling to mark dividing cells. In transgenic mice, the number of proliferating cells was significantly increased distal to the crush site compared to wild-types. We propose that endogenously synthesized FGF-2 influences early peripheral nerve regeneration by regulating Schwann cell proliferation, axonal regrowth, and remyelination.

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“…The reported impact of β-FGF on neurite elongation is mixed. Several studies found that β-FGF promoted axonal elongation without a pronounced effect on axonal branching (Klimaschewski, Nindl, Feurle, Kavakebi, & Kostron, 2004;Mearow & Kril, 1995;Streppel et al, 2002). However, other in vitro studies demonstrated that the neurite growth response to β-FGF was minimal (Kimpinski & Mearow, 2001), either alone or when combined with NGF.…”

Section: Discussionmentioning

confidence: 99%

Neural Precursor Cell Lines Promote Neurite Branching

Neville

Huang

Shyu

et al. 2009

International Journal of Neuroscience

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Schwann cells and primary progenitor cells improve regeneration across peripheral nerve defects. This study examined the impact of immortalized neural precursor cells on regeneration of rat nerve defects. Across 10-mm gaps, neuromas formed without neural cables with C17.2- or RN33B-transplanted cells, but neural cables formed across 5-mm gaps seeded with RN33B cells. In vitro, dorsal root ganglia neurites elongated across Schwann and RN33B cells; RN33B cells induced neurite branching with shorter total outgrowth. Neural cable formation in vivo was likely determined by the balance of guidance and branch-inducing factors secreted by Schwann and transplanted precursor cells.

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Basic fibroblast growth factor isoforms promote axonal elongation and branching of adult sensory neurons in vitro

Cited by 52 publications

References 36 publications

The Pros and Cons of Growth Factors and Cytokines in Peripheral Axon Regeneration

The Pros and Cons of Growth Factors and Cytokines in Peripheral Axon Regeneration

Faster nerve regeneration after sciatic nerve injury in mice over‐expressing basic fibroblast growth factor

Neural Precursor Cell Lines Promote Neurite Branching

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