Basic research and clinical application of beta-tricalcium phosphate (β-TCP)

Tanaka, Takaaki; Komaki, Hirokazu; Chazono, Masaaki; Kitasato, Seiichiro; Kakuta, Atsuhito; Akiyama, Shoshi; Marumo, Keishi

doi:10.1016/j.morpho.2017.03.002

Cited by 67 publications

(52 citation statements)

References 24 publications

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“…The cortical bone defect was only reconstructed when the complex was supplemented with fibroblast growth factor-2 (FGF-2), though the effect occurred 12 weeks postimplantation (Komaki et al, 2006). Relative to cancellous bone, cortical bone regenerates less easily (Komaki et al, 2006;Tanaka et al, 2017). The number of TRAP-positive cells in the 10 −3 M ALN-treated group was significantly lower than that for other groups (*p < .05) F I G U R E 5 New bone formation rate.…”

Section: Discussionmentioning

confidence: 99%

“…A complex of β-TCP granules and either hyaluronic acid or collagen promoted cancellous bone formation (Chazono et al, 2004). Relative to cancellous bone, cortical bone regenerates less easily (Komaki et al, 2006;Tanaka et al, 2017). FGF-2 promotes undifferentiated cell proliferation and vascularization but does not promote differentiation to osteogenic cells (Komaki et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Resorption of β-TCP involves both cell-and solution-mediated disintegration. Previously, we reported that TRAPpositive cells have a substantial role in β-TCP bioresorption, highlighting the importance of β-TCP resorption in the formation of bone (Chazono et al, 2004;Chazono, Tanaka, Kitasato, Kikuchi, & Marumo, 2008;Tanaka et al, 2010;Tanaka et al, 2017). Chappard et al recently shown that both osteoclasts and activated macrophages act together to resorb β-TCP.…”

mentioning

confidence: 87%

“…β-tricalcium phosphate (β-TCP) has gained interest as a possible bone graft substitute owing to its biodegradability and biocompatibility (Chazono, Tanaka, Komaki, & Fujii, 2004;Komaki, Tanaka, Chazono, & Kikuchi, 2006;Tanaka et al, 2008;Tanaka et al, 2017;Walsh et al, 2008). Resorption of β-TCP involves both cell-and solution-mediated disintegration.…”

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confidence: 99%

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Local application of alendronate controls bone formation and β‐tricalcium phosphate resorption induced by recombinant human bone morphogenetic protein‐2

Kitasato

Tanaka

Chazono

et al. 2019

J Biomedical Materials Res

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This study examined the ability of local alendronate (ALN) administration to control β-tricalcium phosphate (β-TCP) resorption as well as the induction of bone formation by recombinant human bone morphogenetic protein-2 (rhBMP-2). A 15-mm criticalsized bone defect was created in the diaphysis of rabbit ulnae. Nine female rabbits (4 to 5 months-old) were divided into 3 groups. Group 1 (n = 6 ulnae) animals received implants consisting of β-TCP granules and 25 μg of rhBMP-2 in 6.5% collagen gel.Group 2 (6 ulnae) and Group 3 (6 ulnae) animals received the same implants, but with 10 −6 M and 10 −3 M ALN-treated TCP granules, respectively. Two weeks postsurgery, tartrate-resistant acid phosphatase-positive cell counts, new bone formation, and residual β-TCP were evaluated. This study showed that a high dose of ALN strongly reduced osteoclastic resorption of β-TCP induced by rhBMP-2, resulting in decreased bone formation. In contrast, a low dose of ALN slightly reduced the bone resorptive effect but increased bone formation. These results suggest that osteoclast-mediated resorption plays an important role in bone formation and a coupling-like phenomenon could occur in the β-TCP-implanted area, and that administration of a low dose of ALN may solve clinical bone resorptive problems induced by rhBMP-2. K E Y W O R D Sbisphosphonate, bone morphogenetic protein, bone regeneration, bone resorption, tricalcium phosphate

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 87%

mentioning

confidence: 99%

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Local application of alendronate controls bone formation and β‐tricalcium phosphate resorption induced by recombinant human bone morphogenetic protein‐2

Kitasato

Tanaka

Chazono

et al. 2019

J Biomedical Materials Res

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“…The prime intention of this refined synthesis of a PLLA/PCL blend was designed to provide a novel type of biomaterial that is suitable for in vitro cell testing, tissue engineering applications, and implantation. The material property testing here was by and large in reference to TCP, a material which is routinely used in various clinical reparative medicinal approaches as well as in tissue engineering approaches [20, 32]. The novel PbP blend is a cream-white, geometrically stable, sturdy and brittle material, and can be sintered to take specific shape thereby taking differently porous structures.…”

Section: Discussionmentioning

confidence: 99%

Hard Tissue Augmentation of Aged Bone by Means of a Tin-Free PLLA-PCL Co-Polymer Exhibiting in vivo Anergy and Long-Term Structural Stability

et al. 2019

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Background: Due to aging, tissue regeneration gradually declines. Contemporary strategies to promote tissue-specific regeneration, in particular in elderly patients, often include synthetic material apt for implantation primarily aiming at upholding body functions and regaining appropriate anatomical and functional integrity. Objective: Biomaterials suitable for complex reconstruction surgical procedures have to exert high physicochemical stability and biocompatibility. Method: A polymer made of poly-L-lactic acid and poly-ε-caprolactone was synthesized by means of a novel tin-free catalytic process. The material was tested in a bioreactor-assisted perfusion culture and implanted in a sheep model for lateral augmentation of the mandible. Histological and volumetric evaluation was performed 3 and 6 months post-implantation. Results: After synthesis the material could be further refined by cryogrinding and sintering, thus yielding differently porous scaffolds that exhibited a firm and stable appearance. In perfusion culture, no disintegration was observed for extended periods of up to 7 weeks, while mesenchymal stromal cells readily attached to the material, steadily proliferated, and deposited extracellular calcium. The material was tested in vivo together with autologous bone marrow-derived stromal cells. Up to 6 months post-implantation, the material hardly changed in shape with composition also refraining from foreign body reactions. Conclusion: Given the long-term shape stability in vivo, featuring imperceptible degradation and little scarring as well as exerting good compatibility to cells and surrounding tissues, this novel biomaterial is suitable as a space filler in large anatomical defects.

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Biomaterial granules used for filling bone defects constitute 3D scaffolds: porosity, microarchitecture and molecular composition analyzed by microCT and Raman microspectroscopy

Arbez

Kün‐Darbois

Convert³

et al. 2018

J Biomed Mater Res

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Biomaterials are used in the granular form to fill small bone defects. Granules can be prepared with a grinder from trabecular bone samples or provided as synthetic biomaterials by industry. Granules occupy the 3D-space and create a macroporosity allowing invasion of vascular and bone cells when the inter-granular pores are larger than 300 µm. We compared the 3D-porosity of granule stacks obtained or prepared with nine biomaterials Osteopure , Lubboc , Bio-Oss , CopiOs , TCP Dental , TCP Dental HP , KeraOs , and TCH in comparison with that of human trabecular bone. For each biomaterial, two sizes of granules were analyzed: 250-1000 and 1000-2000 µm. Microcomputed tomography determined porosity and microarchitectural characteristics of granular stacks and Raman microspectroscopy was used to analyze their composition. Stacks of 250-1000 µm granules had a much lower porosity than 1000-2000 µm granules and the maximum frequency of pores was always centered at 200-250 µm. One biomaterial contained substantial amount of cortical bone (Bio-Oss ). The highest porosity and pore size was obtained with TCP Dental HP. Raman spectroscopy found differences in biomaterials of the same composition. Stacks of granules represent 3D scaffolds resembling trabecular bone with an interconnected porous microarchitecture. Small granules have created pores <300 µm in diameter; this can interfere with vascular colonization. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

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Basic research and clinical application of beta-tricalcium phosphate (β-TCP)

Cited by 67 publications

References 24 publications

Local application of alendronate controls bone formation and β‐tricalcium phosphate resorption induced by recombinant human bone morphogenetic protein‐2

Local application of alendronate controls bone formation and β‐tricalcium phosphate resorption induced by recombinant human bone morphogenetic protein‐2

Hard Tissue Augmentation of Aged Bone by Means of a Tin-Free PLLA-PCL Co-Polymer Exhibiting in vivo Anergy and Long-Term Structural Stability

Biomaterial granules used for filling bone defects constitute 3D scaffolds: porosity, microarchitecture and molecular composition analyzed by microCT and Raman microspectroscopy

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