Behaviour of vegetable phospholipids in thin-layer chromatography

Nanogram Detection of Phospholipids on Thin-Layer Chromatograms

“…Because maximum information can be obtained from a chromatogram by using several reagents in succession, phosphorus-specific stains can be used in several combinations, e.g., before and after the use of an universal lipid spray or ninhydrin (specific for amino groups). Trying various combinations of dye procedures, we found that the approach with the fluorochrome DPH, reported as a nondestructive dye and tool for preparative lipid isolation (8), in conjunction with the widely distributed Dittmer-Lester reagent (9,(15)(16)(17) resulted in tremendous increase of sensitivity for phospholipid evaluation. Therefore, the considerably improved procedure, working on a nanogram scale, might be useful for identification of phospholipids, especially if only limited material, e.g., human tissue (biopsy probes) or patient serum, is available.…”

Separation and purification of phosphatidylcholine and phosphatidylethanolamine from soybean degummed oil residues by using solvent extraction and column chromatography

“…Separations and purifications of the phospholipid classes have been extensively studied by a number of researchers in different academic fields [10][11][12][13][14][15][16][17][18][19][20][21]. Pure PC and PE are usually purified by using various chromatographies, such as TLC [10,11], high performance liquid chromatography (HPLC) [12][13][14][15][16] and low-pressure column chromatography (LPCC) [17][18][19][20][21], etc.…”

“…Pure PC and PE are usually purified by using various chromatographies, such as TLC [10,11], high performance liquid chromatography (HPLC) [12][13][14][15][16] and low-pressure column chromatography (LPCC) [17][18][19][20][21], etc. Amongst these methods, separation efficiency of HPLC is the best since small diameter packing can be used, however, the equipment is expensive and the costs of operation and maintenance are also high.…”

“…The soybean phospholipids were usually separated by TLC, HPLC or LPCC without pre-separation in previous literatures [10,[13][14][15][16]. The phospholipid classes could not be separated by using single solvent due to low resolution.…”

Planar chromatography

“…Analytical OPLC, preparative OPLC, and HPLC with the same phase system were compared for the separation of closely related terpenoids (035). Methods for the coupling of TLC and FTIR spectrometry were reported (036,037). Methylbenactyzium bromide in human urine was determined by TLC and pyrolysis GC (038).…”