Beitr�ge zur Chromatographie organischer Substanzen. VI. Die D�nnschichtchromatographie der Kohlehydrate

A Reliable and Quick Radial Thin Layer Chromatographie Method for the Diagnosis of Mono- and Disaccharide Disorders

“…For each Spot, the terriplate defines triangul r diffusion area in a similar way to the "V" bands method of Preyzt al. (5). As Standard sugars, use 50 g/l Solutions of ribose, xyl se, fructose, glucose, galactose, sucrose, lactose and maitose, which are the most commonly encountered in pathology.…”

Preparative Layer Chromatography

“…Of especial importance are impregnations with reagents forming complexes or addition compounds such as silver nitrate<i96,ii2,iio,26,i98,2oo,2io,227) (bonding silver ions with the TT-electrons of double bonds; thus, usuable for the separation of unsaturated lipids,^^^6) ^is and trans isomers of methyl linolates,^26) hydroxylated fatty acids,<2oo) waxes,^^i^) monoterpenes,<227) sesquiter penes, ^^^^ isomeric cyclododecanes),<2io)) boric acid, sodium borate as weU as sodium arsenate (207,214,215,114,197,198,199) (forming chelates; employed for the separation of vicinal dihydroxy isomers); sodium bisulfite(2) (addition compounds with carbonyl containing groups; applicable for the separation of carbohydrates), and trinitrobenzene (204) and picric acid^^^i) (forming complexes, employed for the separation of poly nuclear aromatics). Of especial importance are impregnations with reagents forming complexes or addition compounds such as silver nitrate<i96,ii2,iio,26,i98,2oo,2io,227) (bonding silver ions with the TT-electrons of double bonds; thus, usuable for the separation of unsaturated lipids,^^^6) ^is and trans isomers of methyl linolates,^26) hydroxylated fatty acids,<2oo) waxes,^^i^) monoterpenes,<227) sesquiter penes, ^^^^ isomeric cyclododecanes),<2io)) boric acid, sodium borate as weU as sodium arsenate (207,214,215,114,197,198,199) (forming chelates; employed for the separation of vicinal dihydroxy isomers); sodium bisulfite(2) (addition compounds with carbonyl containing groups; applicable for the separation of carbohydrates), and trinitrobenzene (204) and picric acid^^^i) (forming complexes, employed for the separation of poly nuclear aromatics).…”

Analysis of the Exudate Produced by Streptococcus mutans SL-1 Colonies on Sucrose-Containing Agar Media

“…For qualitative analysis, 1-and 5-,ul portions of exudate were chromatographed on Whatman 3MM paper (Reeve Angel) with n-butanolacetic acid-water solvent systems (60:15:25 for sugars and 60:20:20 for amino acids, vol/vol/ vol). Parallel chromatograms were developed ovemight and sprayed with alkaline silver nitrate (13), naphthoresorcinol (11), diphenylamine-aniline phosphate (1), and aniline phthalate (EM Laboratories) reagents, respectively, to detect carbohydrates. Ninhydrin (0.2% in nbutanol) was used for amino acids, and methyl red (0.02% in ethanol) and methyl orange (0.02%) were used for acids.…”