Beneficial Effect of α-Tocopherol in Renal Ischemia-Reperfusion in Rats

Abstract

ABSTRACT-We evaluated the effects of =-tocopherol (vitamin E) on the products of lipid peroxidation and serum creatinine levels in a rat model of renal ischemia-reperfusion. The animals were submitted to sham operation or renal ischemia-reperfusion, and they were pretreated with =-tocopherol or the vehicle saline. In four groups, we analyzed the lipid peroxidation products by measuring malondialdehyde and chemiluminescence levels. In the other three groups, we studied the serum creatinine levels after the procedures. In our study, the pretreatment with =-tocopherol reduced significantly the lipid peroxidation of renal cells and renal dysfunction induced by renal ischemia-reperfusion in rats.Keywords: =-Tocopherol, Renal ischemia-reperfusion, Lipid peroxidation Renal ischemia-reperfusion is a necessary procedure in circumstances such as surgical revascularization of renal arteries, surgical treatment of suprarenal aortic aneurysms, partial nephrectomies and renal transplantation. Oxygen free radicals (OFR) were shown to contribute to the cellular damage induced by ischemia-reperfusion, probably due to their lipidic oxidative characteristics, and several agents have been used to minimize the OFR action in renal ischemia-reperfusion (1).=-Tocopherol (vitamin E) is localized in the cell membranes and contributes to their stability and seems to protect the membrane lipids against oxidative damage (2).The purpose of these studies was to investigate the efficacy of = -tocopherol in the reduction of injury induced by OFR in a rat model of renal ischemia-reperfusion.Male Wistar rats weighing 250 to 330 g were randomly assigned to the experiments for analysis of the renal cell lipid peroxidation and measurement of serum creatinine levels. All experiments were approved by the local Committee for Animal Use and Care. The animals received general anesthesia with 50 mg/kg, i.p. of thiopental solution (Cristália, São Paulo, Brazil; 50 mg/ml). Renal ischemia-reperfusion was performed through a left flank incision (2 to 2.5 cm) followed by the dissection of the left renal pedicle so as to expose the renal vessels. Nontraumatic vascular clamps were used to stop blood flow. Reperfusion was established by removing the clamps. The abdominal wall (muscular layer and skin) was closed with 3.0 polypropylene and 4.0 mononylon sutures. The animals were randomly distributed into four groups: I) control group (n = 10): rats pretreated with 0.9% NaCl solution, 1 ml/kg, i.p., given 1 h before the sham-operation; II) ischemia group (n = 9): rats undergoing renal ischemia for 50 min; III) ischemia-reperfusion group (n = 10): rats undergoing renal ischemia for 50 min and reperfusion for 50 min; and IV) =-tocopherol group (n = 10): rats pretreated with =-tocopherol solution (Aldrich, Milwaukee, WI, USA; 30 mg/ ml), 30 mg / kg, i.p., 72, 48, 24, 6 and 1 h before ischemia for 50 min followed by reperfusion for 50 min.Immediately after the procedures, the renal tissue was excised to measure OFR's-induced lipid peroxidation of the renal cell membran...

Alprostadil attenuates inflammatory aspects and leucocytes adhesion on renal ischemia and reperfusion injury in rats

“…Experimental models of ischemia and reperfusion are long described and widely found in the literature 16,[19][20][21][22][23] .…”

“…[13][14][15] Some authors have also studied shorter clamping times with similar reperfusion periods with renal pedicle or single limb artery clamping, suggesting questionable benefit of its use. [17][18][19][20][21] We believe that these conflicting results are related to short clamping periods, some of what could even controversially work as preconditioning protective factor. Another major difference is the potential to create significant IRI, which is long known to be When comparing electrolytes, no statistically significant difference between groups on the levels of Sodium (p>0.05) and Calcium (p>0.05) were found.…”

The Protective Effects of Melatonin and Vitamin E against Renal Ischemia-Reperfusion Injury in Rats

“…[12,13] The protective effects of vitamin E on kidney tissue including renal ischemia reperfusion injury have yielded that it reduces lipid peroxidation and positive effects to antioxidant system status. [1,14,15] In light of the above findings, the present study examined the protective effects of exogenous melatonin and vitamin E supplementation on renal function, antioxidant status, lipid peroxidation production, and histopathological and electron microscopic changes in renal ischemia-reperfusion injury.…”

“…[1] Ischemia of an organ or system results in varying degrees of tissue destruction, depending on the duration and extent of occluded arterial blood flow. Organ transplantation offers the best chance of studying clinical and experimental ischemia-reperfusion injury.…”

Inhaled Hydrogen Gas Therapy for Prevention of Renal Ischemia/Reperfusion Injury in Rat Model

“…Antioxidants have been found to protect renal cells from cellular injury induced by I/R. [22][23][24] The finding of the anti-oxidant effect of hydrogen and its therapeutic value led us to suggest that endogenous hydrogen may play an important physiological role in maintaining homeostasis. Several studies have shown that hydrogen exerts cytoprotective effects after various types of injuries.…”