Benzyladenine induces foliar adventitious shoot formation on young plants of two sugarbeet (Beta vulgaris L.) cultivars

Abstract

INDEX WORDSBeta vulgaris, sugarbeet, adventitious shoots, benzyladenine SUMMARY Benzyladenine (BA) at concentrations from 33-2000 mg/l caused development of adventitious shoots on subsequent leaves of sugarbeet plants when sprayed once onto cotyledon-stage seedlings. Cultivar EL44 produced more adventitious shoots and greater fresh weight reduction in response to BA treatment than cultivar EL40. Related anomalies caused by BA included dissected leaves, and bumps and rods on petioles.

Sugar Beet Micropropagation

“…to induce development of somaclonal variability. Somaclonal variability can be defined as genetic variability that occurs in in vitro conditions, usually in callus cells, call suspensions and in plant regenerating from adventitious meristems (4,43). In sugar beet regenerant deriving from leaf stalks were detected changes in the leaf morphology (8), while the changes in chromosome structure and number were detected in hypocotyl regenerants (24) and in plants regenerating from the callus via organogenesis (44,53).…”

“…Very efficient techniques were developed for hypocotyl, cotyledons, epicotyl, leaf, leaf stalk and dormant shoots. The best results were obtained with flower buds, inflorescences and its parts as well as flower stalks (1,19,43). The highest morphogenetic potential for micropropagation was detected in upper parts of inflorescence ( Fig.…”