Bifidobacterium lemurum sp. nov., from faeces of the ring-tailed lemur (Lemur catta)

Modesto, Monica Marianna; Michelini, Samanta; Stefanini, Ilaria; Sandri, Camillo; Spiezio, Caterina; Pisi, Annamaria; Filippini, Gianfranco; Biavati, Bruno; Mattarelli, Paola

doi:10.1099/ijs.0.000162

Cited by 32 publications

(17 citation statements)

References 39 publications

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“…Bifidobacteria are widespread inhabitants of the mammalian, avian, and insect intestinal tracts (11,13,14,62), yet a large part of the currently recognized bifidobacterial taxa, i.e., 49 species and 9 subspecies, has been isolated from the human gut (15,16). Nonetheless, various ecological attempts have recently been made to survey bifidobacterial populations in other mammals (10,(17)(18)(19)(20)(21), which has resulted in the identification of several scientifically accepted and putative novel bifidobacterial species.…”

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confidence: 99%

Tracking the Taxonomy of the Genus Bifidobacterium Based on a Phylogenomic Approach

Lugli

Milani

Duranti

et al. 2018

Appl Environ Microbiol

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For decades, bacterial taxonomy has been based on molecular biology techniques and comparison of molecular marker sequences to measure the degree of genetic similarity and deduce phylogenetic relatedness of novel bacterial species to reference microbial taxa. Due to the advent of the genomic era, access to complete bacterial genome contents has become easier, thereby presenting the opportunity to precisely investigate the overall genetic diversity of microorganisms. Here, we describe a high-accuracy phylogenomic approach to assess the taxonomy of members of the genus and identify apparent misclassifications in current bifidobacterial taxonomy. The developed method was validated by the classification of seven novel taxa belonging to the genus by employing their overall genetic content. The results of this study demonstrate the potential of this whole-genome approach to become the gold standard for phylogenomics-based taxonomic classification of bacteria. Nowadays, next-generation sequencing has given access to genome sequences of the currently known bacterial taxa. The public databases constructed by means of these new technologies allowed comparison of genome sequences between microorganisms, providing information to perform genomic, phylogenomic, and evolutionary analyses. In order to avoid misclassifications in the taxonomy of novel bacterial isolates, new (bifido)bacterial taxons should be validated with a phylogenomic assessment like the approach presented here.

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confidence: 99%

Tracking the Taxonomy of the Genus Bifidobacterium Based on a Phylogenomic Approach

Lugli

Milani

Duranti

et al. 2018

Appl Environ Microbiol

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“…Gram staining was assessed with individual Gram-stain reagents (Merck Millipore) using cells grown on 220 TPY agar at 37°C under anaerobic conditions for 48 h. Motility assay, catalase and oxidase activities were 221 performed according to Modesto et al [27]. 222…”

Section: Operon 144mentioning

confidence: 99%

“…mammals, birds, ungulates, 83 lagomorphs and rodents) and insect pollinators [1,11,12,15,31]. To date, studies on bifidobacterial 84 distribution in New World monkeys and lemurs have described the presence of twelve novel species 85 belonging to this genus [5,23,[25][26][27]. 86…”

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confidence: 99%

Bifidobacterium aerophilum sp. nov., Bifidobacterium avesanii sp. nov. and Bifidobacterium ramosum sp. nov.: Three novel taxa from the faeces of cotton-top tamarin ( Saguinus oedipus L.)

Michelini

Modesto

Filippini

et al. 2016

Systematic and Applied Microbiology

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“…T showed the highest 16S rRNA gene sequence similarity to B. lemurum DSM 28807 T (99.3 %), isolated by Modesto et al (2015) from the ring-tailed lemur (Lemur catta), and to Bifidobacterium pullorum LMG 21816 T (96.4 %).…”

Section: Strain Lmm_e3mentioning

confidence: 99%

Bifidobacterium eulemuris sp. nov., isolated from faeces of black lemurs (Eulemur macaco)

Michelini

Modesto

Pisi

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Forty-three strains of bifidobacteria were isolated from the faeces of two adult black lemurs, Eulemur macaco. Thirty-four were identified as Bifidobacterium lemurum, recently described in Lemur catta. The nine remaining isolates were Gram-positive-staining, non-spore-forming, fructose-6-phosphate phosphoketolase-positive, microaerophilic, irregular rod-shaped bacteria that often presented Y-or V-shaped cells. Typing techniques revealed that these isolates were nearly identical, and strain LMM_E3T was chosen as a representative and characterized further. T showed a peptidoglycan structure that has not been detected in bifidobacteria so far:Based on the phylogenetic, genotypic and phenotypic data, strain LMM_E3 T represents a novel species within the genus Bifidobacterium, for which the name Bifidobacterium eulemuris sp. nov. is proposed; the type strain is LMM_E3 T

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Bifidobacterium lemurum sp. nov., from faeces of the ring-tailed lemur (Lemur catta)

Abstract: Bifidobacterium lemurum sp. nov., from faeces of the ring-tailed lemur (Lemur catta)

Cited by 32 publications

References 39 publications

Tracking the Taxonomy of the Genus Bifidobacterium Based on a Phylogenomic Approach

Tracking the Taxonomy of the Genus Bifidobacterium Based on a Phylogenomic Approach

Bifidobacterium aerophilum sp. nov., Bifidobacterium avesanii sp. nov. and Bifidobacterium ramosum sp. nov.: Three novel taxa from the faeces of cotton-top tamarin ( Saguinus oedipus L.)

Bifidobacterium eulemuris sp. nov., isolated from faeces of black lemurs (Eulemur macaco)

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