Bio-processing of agro-industrial waste orange peel for induced production of pectinase by Trichoderma viridi; its purification and characterization

Irshad, Muhammad; Anwar, Zahid; Mahmood, Zahed; Aqil, Tahir; Mehmmod, Sajid; Nawaz, Haq

doi:10.5505/tjb.2014.55707

Cited by 33 publications

(14 citation statements)

References 18 publications

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“…Moreover, lower concentrations were more suitable than higher concentrations. The present investigation is in contrary to [32, 34] who reported that high concentrations of carbon stimulate enzyme synthesis. Nitrogen sources are biomass sources for microorganisms that play a significant role in the growth and enzyme production [24].…”

Section: Resultscontrasting

confidence: 99%

Catalytic, kinetic and thermodynamic properties of free and immobilized caseinase on mica glass-ceramics

Ahmed

Saleh

Abdel-Hameed

et al. 2019

Heliyon

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Bacillus megaterium 314 strain was able to utilize agricultural and industrial wastes for metallo-protease production. Orange peel and wheat bran were found as the most suitable carbon and nitrogen sources, respectively. Optimized production process enhanced the enzyme production by 5.1-folds. Glass and glass-ceramic with different particle sizes based on mica were used as inorganic carrier. Protease enzyme was immobilized by covalent bonding and physical adsorption methods on nanoparticle supports. Enzyme physically adsorbed on glass ceramic (particle size 0.71–1.0 mm) had the highest residual activity and the highest immobilization yield. Glass-ceramic was characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Immobilized enzyme exhibited activation energy ( E a ) and deactivation rate constant at 60 °C ( k d ) about 1.29 and 1.46-times, respectively lower than free enzyme. Moreover, adsorbed enzyme had higher energy for denaturation ( E d ), half-life ( t 1/2 ), and decimal reduction time ( D ). The thermodynamic parameters of irreversible thermal denaturation for the protease enzyme indicate that immobilized enzyme had higher enthalpy (ΔH°), free energy (ΔG°), and entropy (ΔS°) than free one. There was a significant improvement in the maximum reaction velocity Vmax (2.5-fold), Michaelis constant Km (1.9-fold), and catalytic efficiency Vmax/Km (4.7-fold) values after immobilization indicating the efficiency and effectiveness of immobilization approach.

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Section: Resultscontrasting

confidence: 99%

Catalytic, kinetic and thermodynamic properties of free and immobilized caseinase on mica glass-ceramics

Ahmed

Saleh

Abdel-Hameed

et al. 2019

Heliyon

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show abstract

“…d). Maximum xylanase and pectinase production from various microbes under SSF using 10%–20% inoculums has been reported by various researchers .…”

Section: Resultsmentioning

confidence: 93%

“…Wheat bran has been reported to be mainly used by many researchers as the best inducer for xylanase and pectinase production . Apart from wheat bran, various other agroresidues such as rice bran , barley bran , corncob , wheat straw , rice straw , sorghum straw , maize straw , paddy husk , cassava bagasse , apple pomace , banana waste , sugarcane baggases , sweet sorghum baggase , and oil‐palm‐empty‐bunches have been used for xylanase production and orange/citrus peel , rice straw, orange pulp , banana peel , sugarcane bagasse , sugar beet pulp , and pineapple residue for pectinase production by different microorganisms.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Kaur

Varghese

Mahajan

2019

Biotech and App Biochem

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Simultaneous production of alkaline xylanase and all seven types of pectinases by a bacterial isolate, under solid-state fermentation was checked in this study. Under optimized conditions, high concurrent production of xylanase (22,800 ± 578 IU/g substrate) and pectinase (4,832 ± 189 IU/g substrate) was achieved. The different types of pectinases produced were exo-polymethylgalacturonase (782 IU/g), endo-polymethylgalacturonase (6.42 U/g), exo-polygalacturonase (2,250 IU/g), endo-polygalacturonase (11.57 U/g), polymethylgalacturonate lyase (53.99 IU/g), polygalacturonate lyase (59.78 IU/g), and pectin esterase (5.78 IU/g). Wheat bran resulted in the highest titer of both enzymes. The maximum xylanase-pectinase yield was detected after 7 days of incubation with 2 mM MgSO 4 and 1.5 g/L K 2 HPO 4 at wheat bran to moisture ratio 1:1.5 (w/v), media to flask volume ratio 1:25, pH 7.0, temperature 37 • C, and inoculum size 15%. Xylanase was most stable at pH 8.0, retained more than 75% activity up to 24 H, whereas pectinase was most stable at pH 9.0, having full activity even after 24 H. At 45 • C, the xylanase showed 82% residual activity after 6 H of incubation. The pectinase was 97% and 61% stable up to 3 H at 50 and 55 • C, respectively. This is the first report showing the production of xylanase-pectinases by bacterium along with high titer of seven types of pectinases, suitable for industries.

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“…Neste sentido, diversos resíduos têm sido estudados -principalmente aqueles gerados a partir de frutas cítricas, como polpa de laranja, além de polpa de maracujá e de uva -visando à redução do custo de produção de enzimas (OLIVEIRA et al, 2011;IRSHAD et al, 2014;KOSER et al, 2014;GERHARDT et al, 2015). Em relação às pectinases, objetiva-se utilizar subprodutos que contenham pectina, servindo como fontes indutoras de baixo custo KOSER et al, 2014).…”

Section: Produção De Pectina Liase Por Linhagens De Fungos Filamentosunclassified

Produção De Pectina Liase Por Linhagens De Fungos Filamentosos Em Polpas De Buriti E Maracujá Como Fontes De Carbono

Farias¹,

Carvalho²,

Machado³

et al. 2015

Enciclopédia Biosfera

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Bio-processing of agro-industrial waste orange peel for induced production of pectinase by Trichoderma viridi; its purification and characterization

Cited by 33 publications

References 18 publications

Catalytic, kinetic and thermodynamic properties of free and immobilized caseinase on mica glass-ceramics

Catalytic, kinetic and thermodynamic properties of free and immobilized caseinase on mica glass-ceramics

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Produção De Pectina Liase Por Linhagens De Fungos Filamentosos Em Polpas De Buriti E Maracujá Como Fontes De Carbono

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