Bioactivation of Plane and Patterned PDMS Thin Films by Wettability Engineering

Scharin, M.; Rommel, Mathias; Dirnecker, T.; Marhenke, J.; Herrmann, Bent; Rumler, Maximilian; Fader, R.; Frey, L.; Herrmann, Matthias

doi:10.1007/s12668-014-0145-6

Cited by 2 publications

(7 citation statements)

References 30 publications

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“…Plane as well as nano- and micrometer-scaled pillar-patterned S-, H-, and X-PDMS growth substrates were fabricated as described in our previous work (Scharin et al, 2014 ). The patterned growth substrates were prepared from Si masters with nominal hole diameter and pitch of 2 and 6 μm, respectively, and nominal hole depths between 130 and 1,800 nm.…”

Section: Methodsmentioning

confidence: 99%

“…The wettability of PDMS substrates was altered by plasma treatment using low frequency plasma (40 kHz) in a PlasmaPrep2 tool (GaLa Instrumente). Plane and pillar-patterned PDMS films were treated for 10 min with forming gas plasma (95% N 2 /5% H 2 , 400 cm 3 /min, 150 W plasma power), since PDMS surfaces treated by N 2 /H 2 plasma have shown super-hydrophilic behavior in previous studies (Scharin et al, 2014 ). Immediately after plasma treatment samples were stored in distilled water to minimize hydrophobic recovery (Scharin et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

“…H-PDMS is a more rigid PDMS type with a comparably higher Young's modulus (8–12 MPa). This material has proven to be suitable for generating sub-micrometer-patterned structures, however, it has also shown to be unsuitable for fabricating nanometer-sized structures with high aspect ratios (Verschuuren, 2010 ; Schmitt et al, 2012 ; Scharin et al, 2014 ). X-PDMS is the stiffest of the three PDMS types with a Young's modulus of 20–80 MPa (Verschuuren, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

“…Plasma treatment is suitable to enhance the wettability of PDMS growth surfaces. However, it has been reported that the positive effect is of short duration and that hydrophobicity recovers within a few hours of exposure to ambient air (room temperature and laboratory humidity conditions) (Owen and Smith, 1994 ; Fritz and Owen, 1995 ; Hillborg and Gedde, 1998 ; Bhushan et al, 2008 ; Bodas et al, 2008 ; Gomathi et al, 2008 ; Ambrosia et al, 2013 ; Scharin et al, 2014 ). Modification of the surfaces' roughness is another approach for altering the wettability of PDMS substrates (Owen and Smith, 1994 ; Hillborg and Gedde, 1998 ).…”

Section: Introductionmentioning

confidence: 99%

“…To this end, we aimed to manufacture various growth substrates using the elastomer types S-, H-, and X-PDMS, and to alter their wettability (i) by plasma treatment and (ii) based on nano- and micrometer scaled three-dimensional surface structuring. Plasma treatment was used to investigate its influence on the wettability of all PDMS types with emphasis on comparing results for X-PDMS being studied for the first time in this context with results from H-PDMS and S-PDMS characterized before (Scharin et al, 2014 ). We further aimed to analyse the roughness of the manufactured PDMS growth substrates using atomic force microscopy (AFM) and to measure the contact angle of water on the fabricated surfaces as an indicator of wettability.…”

Section: Introductionmentioning

confidence: 99%

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Nano- and Micro-Patterned S-, H-, and X-PDMS for Cell-Based Applications: Comparison of Wettability, Roughness, and Cell-Derived Parameters

Scharin-Mehlmann

Häring

Rommel

et al. 2018

Front. Bioeng. Biotechnol.

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Polydimethylsiloxane (PDMS) is a promising biomaterial for generating artificial extracellular matrix (ECM) like patterned topographies, yet its hydrophobic nature limits its applicability to cell-based approaches. Although plasma treatment can enhance the wettability of PDMS, the surface is known to recover its hydrophobicity within a few hours after exposure to air. To investigate the capability of a novel PDMS-type (X-PDMS) for in vitro based assessment of physiological cell properties, we designed and fabricated plane as well as nano- and micrometer-scaled pillar-patterned growth substrates using the elastomer types S-, H- and X-PDMS, which were fabricated from commercially available components. Most importantly, we compared X-PDMS based growth substrates which have not yet been investigated in this context with H- as well as well-known S-PDMS based substrates. Due to its applicability to fabricating nanometer-sized topographic features with high accuracy and pattern fidelity, this material may be of high relevance for specific biomedical applications. To assess their applicability to cell-based approaches, we characterized the generated surfaces using water contact angle (WCA) measurement and atomic force microscopy (AFM) as indicators of wettability and roughness, respectively. We further assessed cell number, cell area and cellular elongation as indirect measures of cellular viability and adhesion by image cytometry and phenotypic profiling, respectively, using Calcein and Hoechst 33342 stained human foreskin fibroblasts as a model system. We show for the first time that different PDMS types are differently sensitive to plasma treatment. We further demonstrate that surface hydrophobicity changes along with changing height of the pillar-structures. Our data indicate that plane and structured X-PDMS shows cytocompatibility and adhesive properties comparable to the previously described elastomer types S- and H-PDMS. We conclude that nanometer-sized structuring of X-PDMS may serve as a powerful method for altering surface properties toward production of biomedical devices for cell-based applications.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nano- and Micro-Patterned S-, H-, and X-PDMS for Cell-Based Applications: Comparison of Wettability, Roughness, and Cell-Derived Parameters

Scharin-Mehlmann

Häring

Rommel

et al. 2018

Front. Bioeng. Biotechnol.

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Combination of direct laser writing and soft lithography molds for combined nano- and microfabrication

et al. 2016

Self Cite

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This work presents a novel approach for combined micro-and nanofabrication based on the local laser exposure of an UV-curing material through a structured mold. The proposed process makes use of the high freedom of design of direct laser writing (DLW) and the high resolution of soft lithography molds (made e.g. from PDMS). By optimizing the exposure process it was possible to fabricate locally defined hierarchical structures with a height of around 16 µm, that are fully covered with nanometer-sized holes using OrmoComp ® . Manual test imprints showed that the fabricated structures can be used for "step & repeat" nanoimprint processes. Furthermore, the local transfer of nanostructures into two different soft lithography resists (Katiobond 110707, mr-NIL210) was investigated. Diffusion of resist components into the PDMS mold was observed and could be prohibited by the use of hybrid molds, which employ OrmoComp ® as structure containing layer. First experiments revealed successful transfer of the mold's nanostructures into mr-NIL210 but still leave room for improvement concerning the process parameters.

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Bioactivation of Plane and Patterned PDMS Thin Films by Wettability Engineering

Cited by 2 publications

References 30 publications

Nano- and Micro-Patterned S-, H-, and X-PDMS for Cell-Based Applications: Comparison of Wettability, Roughness, and Cell-Derived Parameters

Nano- and Micro-Patterned S-, H-, and X-PDMS for Cell-Based Applications: Comparison of Wettability, Roughness, and Cell-Derived Parameters

Combination of direct laser writing and soft lithography molds for combined nano- and microfabrication

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