Biochemical and biophysical analysis of the intracellular lipid binding proteins of adipocytes

Abstract

analysis of the intracellular lipid binding proteins of adipocytes" (1999). Biochemistry --Faculty Publications. 17.

Modulation of Cutaneous Fatty Acid-binding Protein mRNA Expression in Rat Adipose Tissues by Hereditary Obesity and Dietary Fats

“…C-FABP has also been detected in adipose tissue in mice 13,16,17,22) , though at much lower levels than ALBP. It has been demonstrated that targeted disruption of the ALBP gene in mice provides significant protection from hyperinsulinemia and insulin resistance in the context of both dietary 33) and genetic 34) obesity.…”

“…However, its mRNA was later found to be expressed in various tissues including adipose tissue 5,8,9,[12][13][14][15][16][17][18][19][20][21][22][23] . Krieg et al 5) examined levels of C-FABP mRNA in mouse tissues, and found that the mRNA was most abundant in stratified epithelia of the epidermis and tongue.…”

“…ALBP is the most abundant fatty acid-binding protein in adipose tissue 1,13,16,17,22) . C-FABP has also been detected in adipose tissue in mice 13,16,17,22) , though at much lower levels than ALBP.…”

Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals

“…Adipose tissue FABP quantification in a direct comparison of lean and obese subjects is required to confirm this observation but this was unfortunately beyond the scope of the present investigation. ALBP is extremely abundant in adipocytes, constituting 1±5 % of total soluble protein [15], therefore even the relatively modest percentage-wise increase in ALBP protein in subcutaneous compared to omental adipose tissue from obese subjects represents a large absolute change in ALBP mass between the two sites. Although the isolated subcutaneous adipocytes were significantly larger than omental adipocytes, the differences in ALBP RNA and protein expression measured in intact tissue pieces from the two depots are not likely to be simply a reflection of cell size.…”

Time-Dependent Effects of a High-Energy-Yielding Diet on the Regulation of Specific White Adipose Tissue Genes

“…In this sense, a very significant correlation between PPAR␥2 and UCP3 mRNA expression was detected when all experimental groups were analyzed together. In addition, because aP2 facilitates intracellular mobilization of FFA (43,44), a possible association among PPAR␥2, UCP3 and aP2 in white adipose tissue may be suggested. This relationship was supported by the significant correlations found between PPAR␥2, UCP3 and aP2, which is known to be modulated by PPAR␥2 (37,45).…”