Biochemical studies of the renal radiopharmaceutical compound dimercaptosuccinate

Vanlić-Razumenić, Nada; Petrović, Jelena

doi:10.1007/bf00253168

Cited by 17 publications

(3 citation statements)

References 13 publications

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“…One hour after intravenous injection of labeled DMSA, kidney tissue homogenate preparations were subjected to differential subfractionation to obtain cell organelles. Radioactivity distribution in relation to total radioactivity of kidney homogenate obtained in five repeated experiments was similar to our results [ 27 , 28 ].…”

Section: Discussionsupporting

confidence: 90%

“…Other radiopharmaceuticals, such as 99m Tc-methoxyisobutylisonitrile (MIBI) and 99m Tc-dimercaptosuccinic acid (DMSA), have had their binding sites studied before [ 27 , 28 , 37 ]. Similar to our results, data from whole heart preparations, largely derived from differential centrifugation techniques, indicated that most of 99m Tc-MIBI appears to be associated with the cytosolic fraction.…”

Section: Discussionmentioning

confidence: 99%

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99m-Technetium binding site in bone marrow mononuclear cells

et al. 2015

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IntroductionThe increasing interest in 99m-technetium (99mTc)-labeled stem cells encouraged us to study the 99mTc binding sites in stem cell compartments.MethodsBone marrow mononuclear cells were collected from femurs and tibia of rats. Cells were labeled with 99mTc by a direct method, in which reduced molecules react with 99mTc with the use of chelating agents, and lysed carefully in an ultrasonic apparatus. The organelles were separated by means of differential centrifugation. At the end of this procedure, supernatants and pellets were counted, and the percentages of radioactivity (in megabecquerels) bound to the different cellular fractions were determined. Percentages were calculated by dividing the radioactivity in each fraction by total radioactivity in the sample. The pellets were separated and characterized by their morphology on electron microscopy.ResultsThe labeling procedure did not affect viability of bone marrow mononuclear cells. Radioactivity distributions in bone marrow mononuclear cell organelles, obtained in five independent experiments, were approximately 38.5 % in the nuclei-rich fraction, 5.3 % in the mitochondria-rich fraction, 2.2 % in microsomes, and 54 % in the cytosol. Our results showed that most of the radioactivity remained in the cytosol; therefore, this is an intracellular labeling procedure that has ribosomes unbound to membrane and soluble molecules as targets. However, approximately 39 % of the radioactivity remained bound to the nuclei-rich fraction. To confirm that cell disruption and organelle separation were efficient, transmission electron microscopy assays of all pellets were performed.ConclusionsOur results showed that most of the radioactivity was present in the cytosol fraction. More studies to elucidate the mechanisms involved in the cellular uptake of 99mTc in bone marrow cells are ongoing.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

99m-Technetium binding site in bone marrow mononuclear cells

et al. 2015

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“…Tracer uptaken by these cells is reportedly bound to cytoplasmic proteins; this binding is important for retaining the compound intracellularly [19]. Our findings suggest that, in ITP, the depletion of these binding sites leads to the diffusion of 99m Tc-DMSA out of the cells.…”

Section: Discussionmentioning

confidence: 76%

Poor Renal Accumulation of <sup>99m</sup>Tc-DMSA in Idiopathic Tubular Proteinuria

Suzuki¹,

Suzuki²,

Kume³

et al. 1999

Nephron

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In Japanese patients idiopathic tubular proteinuria presents mainly as asymptomatic tubular low molecular weight proteinuria. This disease has recently been shown to resemble Dent’s disease which is characterized by tubular proteinuria, hypercalciuria, rickets and eventual renal failure. We report on 4 children with idiopathic tubular proteinuria. Although they had normal renal function, as evidenced by serum creatinine or creatinine clearance, they had very poor renal accumulation of ^99mTc-DMSA and the presence of large amounts of tracer in the bladder. Additionally, the patient with the largest amounts of tubular proteinuria had the poorest renal accumulation of the 4 patients. The renal accumulation of tracer decreased with time from a maximum at 10 min after injection. These findings demonstrate that the tracer, once taken to be confined to the proximal tubular cells, is immediately excreted to the tubular lumen. We suggest that poor renal accumulation of ^99mTc-DMSA is very important in elucidating the mechanism of idiopathic tubular proteinuria, and that ^99mTc-DMSA renoscintigraphy is useful in the evaluation of the patient’s renal function over time.

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Biochemical studies of the renal radiopharmaceutical compound dimercaptosuccinate

Vanlić-Razumenić

Petrović²

1981

Eur J Nucl Med

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Subcellular distribution of 99mTc-DMS (dimercaptosuccinic acid) complex in the rat kidney has been studied. One hour after IV injection of labelled DMS preparation kidney tissue homogenate was subjected to differential subfractionation to obtain cell organelles. Radioactivity distribution in relation to total radioactivity of kidney homogenate obtained in five repeated experiments was as follows (+/- SD): nuclei 4.56% (+/- 0.7); mitochondria 5.97% (+/- 0.7); microsomes 3.63% (+/- 0.8) and cytosol 30.92% (+/- 1.8). Specific activities expressed as counts/min/mg of protein were 148,520 (+/- 15%), 97,440 (+/- 17.7%) and 76,180 (+/- 29%) for mitochondria, cytosol and microsomes respectively. Specific activity of the nuclei was 230.060 (+/- 27%) counts/min/mg of DNA. These results show without doubt that this renal imaging agent, successfully used in human medicine, penetrates into kidney cells where it binds mostly to soluble cytoplasmic proteins and mitochondria and to a lesser extent, to both microsomes and nuclei.

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Biochemical studies of the renal radiopharmaceutical compound dimercaptosuccinate

Cited by 17 publications

References 13 publications

99m-Technetium binding site in bone marrow mononuclear cells

99m-Technetium binding site in bone marrow mononuclear cells

Poor Renal Accumulation of <sup>99m</sup>Tc-DMSA in Idiopathic Tubular Proteinuria

Biochemical studies of the renal radiopharmaceutical compound dimercaptosuccinate

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