Biocompatibility of four root canal sealers: a histopathological evaluation in rat subcutaneous connective tissue

Silveira, Camila Maggi Maia; Pinto, Shelon Cristina Souza; Zedebski, Rosário de Arruda Moura; Santos, Fábio André dos; Pilatti, Gibson Luiz

doi:10.1590/s0103-64402011000100003

Cited by 38 publications

(44 citation statements)

References 22 publications

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“…Zinc oxide-eugenol paste served as positive control because it is known as an irritating material for the tissues. The irritating potential of zinc oxide-eugenol has been extensively demonstrated, characterized by the formation of areas of tissue destruction and presence of severe inflammation (18,22,24,25) and discrete presence of newly formed connective tissue (24). The results of the present study are similar to previous findings, since the inflammation levels were minimal in NC and high in PC.…”

Section: Discussionsupporting

confidence: 90%

“…More specifically, implantation of materials in the subcutaneous tissue of small animals like rats is considered a reliable and adequate test to evaluate the biocompatibility of materials (17,18,(21)(22)(23). The material may be introduced in the tissues inside polyethylene tubes (17).…”

Section: Discussionmentioning

confidence: 99%

“…The material may be introduced in the tissues inside polyethylene tubes (17). The use of tubes is recommended because they are not irritant to the tissues (17,18,(22)(23)(24)(25) and prevent material diffusion in the tissues, simulating the clinical conditions of medications applied in the root canal (23).…”

Section: Discussionmentioning

confidence: 99%

“…Silveira et al (22) stated that a material is considered biocompatible if the inflammatory reaction is reduced to insignificant along with time.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, empty tube served as negative control because it is an inert material and allows for the formation of connective tissue in contact with its surface (22)(23)(24)(25). Zinc oxide-eugenol paste served as positive control because it is known as an irritating material for the tissues.…”

Section: Discussionmentioning

confidence: 99%

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Biocompatibility of a Calcium Hydroxide-Propolis Experimental Paste in Rat Subcutaneous Tissue

et al. 2014

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Intracanal medications are fundamental for disinfection of the root canal system and participate in periapical repair, so their biocompatibility is of utmost importance to avoid tissue damage. This study evaluated the biocompatibility of a experimental paste of calcium hydroxide and propolis in the subcutaneous tissue of rats. The study was conducted on 15 male Wistar rats. Two incisions were made on the dorsal region of each animal for introduction of 4 tubes: one tube was empty; one contained zinc oxide-eugenol cement, and the two other tubes were filled with experimental paste. After 7, 14 and 30 days, the animals were euthanized and the specimens were subjected to histotechnical preparation. The hematoxylin and eosin-stained histological sections were analyzed by light microscopy. Scores were established according to the inflammatory process and statistically compared by the Tukey test (α=5%). The analysis of histological sections showed non-significant or mild inflammatory reaction in the connective tissue in contact with the empty tubes in all study periods while the contact of subcutaneous tissue with zinc oxide-eugenol elicited moderate or severe inflammation similarly without significant difference among the study periods. The connective tissue was moderately inflamed at 7 days when contacting the experimental paste, but the inflammatory process was non-significant or mild at 14 and 30 days. The experimental paste was biocompatible with the tissues after 14 days of subcutaneous implantation.

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