Biocompatibility of New Calcium Aluminate Cement: Tissue Reaction and Expression of Inflammatory Mediators and Cytokines

Garcia, Lucas da Fonseca Roberti; Huck, Cláudia; Oliveira, Letícia Menezes de; Souza, Pedro Paulo Chaves de; Costa, Carlos Alberto de Souza

doi:10.1016/j.joen.2014.08.015

Cited by 27 publications

(29 citation statements)

References 23 publications

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“…Garcia et al (20) evaluating the local biocompatibility of calcium aluminate cement, observed an inflammatory response in the subcutaneous tissue of rats with a pattern similar to the one obtained in the present study. This was also observed when the calcium aluminate cement was used for filling bone defects, with continuous reduction in the amplitude of the inflammatory infiltrate and linear closure of the experimental defects along the times of analysis (12).…”

Section: Repair Of Bone Defectssupporting

confidence: 88%

“…Saghiri et al (19) reported that inflammatory infiltrate tended to diminish with the course of time, and the higher the medium pH, the more evident would be the inflammatory response. With time, the calcium aluminate tended to stabilize the medium pH, turning it alkaline due to the release of Ca 2+ ions (13), which produced a favorable environment for tissue repair (13,20). This may have been crucial for the inflammatory response over the course of the experimental time intervals, and for the pattern of tissue repair observed in the groups submitted to implantation of the biomaterials and the negative control group (blood clot), which resulted in significant difference in the final period of analysis.…”

Section: Repair Of Bone Defectsmentioning

confidence: 99%

“…The great advantage of this new class of biomaterials is their capacity for stimulating early vascular invasion (20). Thus, in the other time intervals (15 and 30 days) there was formation of a bone tissue with the tendency to linear closure of the defect.…”

Section: Repair Of Bone Defectsmentioning

confidence: 99%

“…On the other hand, the scaffold group presented similar results to the group submitted to bone graft, in the final period of analysis. This fact could be explained mainly by the differences observed in the biomaterials' architecture and porosity (11,20). According to the required bone repair to be made, these differences in composition may interfere in a positive or negative manner in the velocity of the tissue new bone formation process (11,20).…”

Section: Repair Of Bone Defectsmentioning

confidence: 99%

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Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement

et al. 2017

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Innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. The purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. Eighteen New Zealand white rabbits (Oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). Four bone defects were created in the rabbits calvaria, which were individually filled with the biomembrane, scaffold, blood clot (negative control) and autologous bone (positive control). Histopathological analysis was performed using optical microscope at 32´, 64´, 125´ and 320´ magnifications. Cell response to inflammation and new bone tissue formation was quantified using a score system. The biomembrane group presented greater inflammatory response at 15 days, with significant difference to autologous bone group (p<0.05). There was no statistically significant difference for foreign body type reaction among groups (p>0.05). Concerning new bone formation, linear closure of the defect area was observed more evidently in the group with autologous bone. The scaffold group presented similar results compared with the autologous bone group at 30 days (p>0.05). Both tested biomaterials presented similar biocompatibility compared with the control groups. In addition, the biomembrane and scaffold presented similar osteoinductive capacity, stimulating bone repair process in the course of the experimental time intervals.

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Section: Repair Of Bone Defectssupporting

confidence: 88%

Section: Repair Of Bone Defectsmentioning

confidence: 99%

Section: Repair Of Bone Defectsmentioning

confidence: 99%

Section: Repair Of Bone Defectsmentioning

confidence: 99%

See 2 more Smart Citations

Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement

et al. 2017

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“…However, various in vivo studies that assessed the biocompatibility of this cement (21,22) demonstrated that after 30 days, the material allowed adequate tissue repair, indicating that the alkaline pH produced by calcium ion release was maintained (22,23). It is believed that this ability to maintain the alkaline pH of the medium is the main responsible for the excellent biological performances of MTA and EndoBinder (21).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of New Calcium Aluminate Cement (EndoBinder) Containing Different Radiopacifiers

et al. 2017

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This study aimed to evaluate the cytotoxicity of a calcium aluminate cement (EndoBinder) containing different radiopacifiers, Bi 2 O 3 , ZnO or ZrO 2 , compared with Mineral Trioxide Aggregate (MTA). According to ISO 10993-12:2012 (E) recommendations, 0.2 g of each cement were applied in transwell inserts and placed in 24-well culture plates containing 1 mL of culture medium (DMEM). After 24 h of incubation, the extracts (DMEM containing components released from the cements) were applied to immortalized odontoblast-like MDPC-23 cells. Cell viability (MTT test), alkaline phosphatase activity (ALP), total protein production and cell morphology (Scanning Electron Microscopy -SEM) were evaluated. The volume of 50 µL of extract was used to determine the chemical elements released by the cements using Energy Dispersive Spectroscopy (EDS). The following groups were established (n=6): NC -negative control (without treatment); EB -EndoBinder without radiopacifier; EBBO -EndoBinder+Bi 2 O 3 ; EBZnO -EndoBinder+ZnO; EBZrOEndoBinder+ZrO 2 and WMTA -White MTA. Data were subjected to statistical analysis (Kruskal-Wallis test, level of significance=5%). Cells exposed to the different versions of EndoBinder presented small reduction in viability, total protein production and ALP activity, with values similar to the NC and WMTA groups (p>0.05). Different elements (C, O, Na, Al, P, Si, Cl, Bi, K) released by the cements were detected in the extracts. However, the cells had no significant changes in their morphology. EndoBinder and MTA did not affect negatively the metabolism of the odontoblastic-like cells, showing it to be cytocompatible, irrespective of the used radiopacifier.

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Root‐End Filling and Perforation Repair Materials and Techniques

Camilleri

Pertl

2021

Endodontic Materials in Clinical Practice

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Biocompatibility of New Calcium Aluminate Cement: Tissue Reaction and Expression of Inflammatory Mediators and Cytokines

Cited by 27 publications

References 23 publications

Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement

Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement

Cytotoxicity of New Calcium Aluminate Cement (EndoBinder) Containing Different Radiopacifiers

Root‐End Filling and Perforation Repair Materials and Techniques

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