Biocompatibility of particulate polymethylmethacrylate bone cements: a comparative study in vitro and in vivo

Thomson, LA; Law, Frances; James, K.; Matthew, Cara; Rushton, Neil

doi:10.1016/0142-9612(92)90173-l

Cited by 47 publications

(21 citation statements)

References 11 publications

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“…Together with mechanical factors, biological reactions of bone cells to the components of cement have been implicated [3,4]. Both the unreacted monomer, which is entrapped within the tissue and exerts local toxic effects [5], as well as benzoyl peroxide and paratoluidine or other aromatic amines have been shown to provoke damage to the tissues [6,7]. Antibiotics and radio-opaci ers may also be responsible for undesired effects.…”

Section: Introductionmentioning

confidence: 97%

In vitro testing of ten bone cements after different time intervals from polymerization

Ciapetti¹,

Granchi²,

Stea³

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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Biological response of cells to implanted bone cement is a fundamental but often neglected issue in successful cemented implants. In this study, ten acrylic bone cements for orthopedics were assayed using two different in vitro testing methods on L929 cells. The cements were mixed as prescribed, cured for either 1 h or 7 days and then extracted in minimum essential medium (MEM) according to the ISO standard for the preparation of samples. For the evaluation of cytotoxicity, the neutral red uptake assay (NRU) and the incorporation of propidium iodide (PI) were used to detect the viability/death of cells. The two methods were shown to be well correlated (p < 0.0001) in the case of both the 1-h and the 7-day extracts. Two cements, i.e. CERIM LT and CMW2, were found to be toxic after 1-h curing through both the spectrophotometric NRU assay and the cytofluorometric assay with PI. After 7-day curing, these two cements, as well as the Zimmer-low viscosity cement, were toxic according to the NRU assay. The toxic effect of all the cements disappeared after dilution of extracts 1:2 with MEM, except in the case of CERIM LT. In the search for the component inducing the toxic effect, the possible contribution of the residual monomer was discarded on the basis of literature data and the influence of various other factors was analyzed, including the contrast medium (barium sulphate or zirconium dioxide) and the concentrations of N,N-dimethyl-paratoluidine and of benzoyl peroxide (< 1% or > or = 1%). Unlike zirconium dioxide, barium sulphate was found to damage the cells at the 1-h endpoint. Benzoyl peroxide at concentration > or = 1% was found to affect cells at the same endpoint, whereas dimethylparatoluidine had no effect regardless of the proportion.

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Section: Introductionmentioning

confidence: 97%

In vitro testing of ten bone cements after different time intervals from polymerization

Ciapetti¹,

Granchi²,

Stea³

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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“…Polymethylmetacrylate -PMMA -is characterized by excellent biocompatibility with low intrinsic toxicity and inflammatory activation [7], but experience has shown that not all antibiotics have the properties necessary for their incorporation in this cement. In this context, aminoglycosides and glycopeptides (vancomycin) are known to be the two groups of antibiotics that satisfy the optimal criteria to be included in these cements (availability in powder form, wide antibacterial spectrum, bactericidity at low concentrations, elution from PMMA in high concentrations for prolonged periods, thermal stability, low or no risk of allergy or delayed hypersensitivity, low influence on the mechanical properties of the cement, and low serum protein binding) [8].…”

mentioning

confidence: 99%

Study of the Influence of Bone Cement Type and Mixing Method on the Bioactivity and the Elution Kinetics of Ciprofloxacin

Martínez-Moreno

Mura

Merino

et al. 2015

The Journal of Arthroplasty

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“…The biological responses to wear debris have been studied extensively over the last two decades (Willert 1977;Rushton and Rae 1984;Jasty et al 1986;Ohlin, Johnell and Lerner 1990;Thomson et al 1992;Harris 1994). There is good evidence that particles of a variety of metals, ceramics and polymers can damage human cells in vitro and may incite foreign-body reactions (Rae 1975;Maloney et al 1993) or bone resorption in vivo (Murray and Rushton 1990;Ohlin et al 1990;Haynes et al 1993).…”

mentioning

confidence: 99%

The Effects of Particulate Polyethylene at a Weight-Bearing Bone-Implant Interface

Allen

Brett

Millett

et al. 1996

The Journal of Bone and Joint Surgery. British volume

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In ten male rats we inserted ceramic 'drawing-pin' implants in weight-bearing positions within the right proximal tibia. Two animals were killed 6 weeks after surgery and two more 14 weeks after surgery. The remaining six received intra-articular injections of either high-density polyethylene (4 rats) or saline (2 rats) at 8, 10 and 12 weeks after surgery. These animals were killed two weeks after the last injection.Histological examination of the bone-implant interface in the control animals showed appositional bone growth around the implant at both 6 and 14 weeks. Polyethylene, but not saline, caused a chronic inflammatory response with numerous foreign-body giant cells in periprosthetic tissues.Our model of a stable, weight-bearing bone-implant interface provides a simple and reliable system in which to study in vivo the effects of particulate materials used in orthopaedic surgery.

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Biocompatibility of particulate polymethylmethacrylate bone cements: a comparative study in vitro and in vivo

Cited by 47 publications

References 11 publications

In vitro testing of ten bone cements after different time intervals from polymerization

In vitro testing of ten bone cements after different time intervals from polymerization

Study of the Influence of Bone Cement Type and Mixing Method on the Bioactivity and the Elution Kinetics of Ciprofloxacin

The Effects of Particulate Polyethylene at a Weight-Bearing Bone-Implant Interface

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