Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense)

Hapida, Yustina; Elfita, Elfita; Widjajanti, Hary; Salni, Salni

doi:10.13057/biodiv/d221253

Cited by 12 publications

(10 citation statements)

References 37 publications

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“…The inoculants were incubated for 3-14 days at room conditions. Purification of fungi endophyte by transferring the colonies to a new petridish containing media and incubating at room temperature for 48 hours (Setiawan, 2022;Hapida et al, 2021).…”

Section: Sample Preparation and Isolation Of Endophytic Fungimentioning

confidence: 99%

Antibacterial and Antioxidant Activity of Endophytic Fungi Extract Isolated from Leaves of Sungkai (Peronema canescens)

Oktiansyah

Widjajanti

Setiawan

et al. 2023

Sci. Technol. Indones

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Sungkai is a plant that is widely found in Indonesia. This plant is often used in traditional medicine so the biotechnology of endophytic fungi is highly needed due to natural resources from plants that have been reduced. This study reported on endophytic fungi found in sungkai leaves and the compound produced. Morphological and molecular identification through phylogenetic tree analysis was carried out to determine the endophytic fungal species found. Potato Dextrose Broth media was used for the cultivation process. Ethyl acetate was used as a solvent for the extraction and the evaporation process used a rotary evaporator. Antioxidant and antibacterial tests were carried out using the DPPH method and paper disc diffusion. Chromatographic techniques were used to isolate the compound and spectroscopic analysis was performed to identify its chemical structure. The results of the morphological and molecular analysis showed Trichoderma asperellum as an endophytic fungus identified. The pure compound obtained from this endophytic fungus was 4-hydroxybenzoic acid. The antioxidant and antibacterial activity showed a strong category (IC50 = 43.88 ????g/mL; MIC 64 ????g/mL). This compound was very likely to be a raw material for new antibiotics and antioxidants through further research with various modifications.

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Section: Sample Preparation and Isolation Of Endophytic Fungimentioning

confidence: 99%

Antibacterial and Antioxidant Activity of Endophytic Fungi Extract Isolated from Leaves of Sungkai (Peronema canescens)

Oktiansyah

Widjajanti

Setiawan

et al. 2023

Sci. Technol. Indones

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“…After incubation, the inhibition zone was observed and measured using a caliper. Determination of the criteria for antibacterial activity using the following formula: 48 Strong:…”

Section: Extraction and Cultivationmentioning

confidence: 99%

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from The Leaves of Sungkai (Peronema canescens)

2023

TJNPR

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Sungkai (Peronema canescens) is a medicinal plant widely used in Indonesia. Its leaves are believed to have the potential to treat fever and boost the immune system. This study investigated the antioxidant and antibacterial activity of endophytic fungi extracts obtained from Sungkai leaves. This will provide basic information for developing the potential of natural ingredients as antioxidants and antibacterials. Endophytic fungi residing in Sungkai leaves were identified morphologically. The antioxidant test was completed using DPPH, while the antibacterial activity was tested using the paper disk diffusion method. Most potential endophytic fungal isolates were identified through molecular identification, and the isolation of a bioactive compound was achieved using column chromatography. The structure of the compound was determined using 1D and 2D NMR spectroscopy methods. Four endophytic fungi isolates were observed in the Sungkai leaves (code RND1–RND4). RND3 demonstrated very strong antioxidant activity (IC50 < 20 µg/mL) and strong antibacterial activity. Based on the molecular test, RND3 was identified as Aspergillus niger. The pure compound isolated from RND3 revealed weak and strong antioxidant (IC50 > 500 µg/mL) and antibacterial (MIC ≤ 64 µg/mL) properties, respectively. Spectroscopic analysis showed that compound 1 was 3-benzyl-2,6-dihydroxy-1,4,11,13-tetramethyl-5- methylene-12,15-dioxo-14-oxabicycloheptadeca-8,16-diene-7-carboxylic acid. Through further research, this study can be used as a basis for the development of compounds as raw materials for drugs.

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“…Furthermore, ethyl acetate was added to the media (1:1) and extracted. Finally, the ethyl acetate extract was separated to be evaporated using a rotary evaporator, and the extract was concentrated using an oven at 45°C (Hapida et al 2021;Oktiansyah et al 2023b).…”

Section: Extraction and Cultivationmentioning

confidence: 99%

Diversity and antioxidant activity of endophytic fungi isolated from salam (Syzygium polyanthum)

et al. 2023

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Abstract. Widjajanti H, Elfita, Sari MT, Hidayati N, Hariani PL, Setiawan A. 2023. Diversity and antioxidant activity of endophytic fungi isolated from salam (Syzygium polyanthum). Biodiversitas 24: 3051-3062. Salam (Syzygium polyanthum (Wight) Walp.) is a medicinal plant from the family Myrtaceae. The leaves are used as a spice and treat diseases such as diabetes mellitus, hypertension, ulcers, diarrhea, gastritis, and skin. This study aimed to determine endophytic fungi's diversity and antioxidant activity in the S. polyanthum plant. In addition, the distribution of endophytic fungi found on S. polyanthum was compared to that of previously reported endophytic fungi on other species of Syzygium. The distribution pattern of these endophytic fungi is needed to determine the presence of potential endophytic fungi that can live in host plants of the same genus. The endophytic fungi were isolated from fresh root bark, stem bark, and leaf tissues of S. polyanthum. The endophytic fungi were identified morphologically to determine their level of diversity; their extract was then tested for their antioxidant activity using the DPPH method. The extract that showed the highest antioxidant activity was identified molecularly. A total of 18 endophytic fungi were obtained from S. polyanthum, including seven isolates from root bark (HSA1–HSA7), four isolates from stem bark (HSB1–HSB4), and seven isolates from leaves (HSD1–HSD7). The morphological identification showed seven genera scattered in all investigated parts: Trichoderma, Penicillium, Aspergillus, Pythium, Papulaspora, Pythiogeton, and Clonostachys. The HSD5 isolate showed the best antioxidant activity, and the molecular identification confirmed this isolate as Clonostachys rosea. The comparison of the distribution of endophytic fungi isolated from S. polyanthum to endophytic fungi isolated from the host genus Syzygium showed that endophytic fungi of three genera, namely Clonostachys, Papulaspora, and Pythiogeton, are specifically found on S. polyanthum. In addition, those of four other genera, namely Trichoderma, Aspergillus, Penicillium, and Pythium, are found on other species of Syzygium.

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Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense)

Cited by 12 publications

References 37 publications

Antibacterial and Antioxidant Activity of Endophytic Fungi Extract Isolated from Leaves of Sungkai (Peronema canescens)

Antibacterial and Antioxidant Activity of Endophytic Fungi Extract Isolated from Leaves of Sungkai (Peronema canescens)

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from The Leaves of Sungkai (Peronema canescens)

Diversity and antioxidant activity of endophytic fungi isolated from salam (Syzygium polyanthum)

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