Bioinformatic analysis of the potential molecular mechanism of PAK7 expression in glioblastoma

Wang, Xuefeng; Liu, Shuang; Shao, Zhengkai; Zhang, Penghai

doi:10.3892/mmr.2020.11206

Cited by 4 publications

(5 citation statements)

References 54 publications

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“…Xue et al analyzed signaling pathways in GBM data compared to normal brain tissue, and found overlapping signaling pathways from gene and miRNA expression, which included ion transport, positive regulation of macromolecule metabolic process, cell cycle and axon guidance as the main enriched signaling pathways [ 36 ]. Similarly, differentially expressed genes were also found, by Wang et al, that are involved in enriched signaling pathways, such as axon guidance [ 37 ].…”

Section: Discussionmentioning

confidence: 95%

Antibody Profiling and In Silico Functional Analysis of Differentially Reactive Antibody Signatures of Glioblastomas and Meningiomas

Milchram

Kulovics

Sonntagbauer

et al. 2023

IJMS

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Studies on tumor-associated antigens in brain tumors are sparse. There is scope for enhancing our understanding of molecular pathology, in order to improve on existing forms, and discover new forms, of treatment, which could be particularly relevant to immuno-oncological strategies. To elucidate immunological differences, and to provide another level of biological information, we performed antibody profiling, based on a high-density protein array (containing 8173 human transcripts), using IgG isolated from the sera of n = 12 preoperative and n = 16 postoperative glioblastomas, n = 26 preoperative and n = 29 postoperative meningiomas, and n = 27 healthy, cancer-free controls. Differentially reactive antigens were compared to gene expression data from an alternate public GBM data set from OncoDB, and were analyzed using the Reactome pathway browser. Protein array analysis identified approximately 350–800 differentially reactive antigens, and revealed different antigen profiles in the glioblastomas and meningiomas, with approximately 20–30%-similar and 10–15%-similar antigens in preoperative and postoperative sera, respectively. Seroreactivity did not correlate with OncoDB-derived gene expression. Antigens in the preoperative glioblastoma sera were enriched for signaling pathways, such as signaling by Rho-GTPases, COPI-mediated anterograde transport and vesicle-mediated transport, while the infectious disease, SRP-dependent membrane targeting cotranslational proteins were enriched in the meningiomas. The pre-vs. postoperative seroreactivity in the glioblastomas was enriched for antigens, e.g., platelet degranulation and metabolism of lipid pathways; in the meningiomas, the antigens were enriched in infectious diseases, metabolism of amino acids and derivatives, and cell cycle. Antibody profiling in both tumor entities elucidated several hundred antigens and characteristic signaling pathways that may provide new insights into molecular pathology and may be of interest for the development of new treatment strategies.

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Section: Discussionmentioning

confidence: 95%

Antibody Profiling and In Silico Functional Analysis of Differentially Reactive Antibody Signatures of Glioblastomas and Meningiomas

Milchram

Kulovics

Sonntagbauer

et al. 2023

IJMS

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“…Research by Huang et al showed that PNMA5 plays an oncogene role in NSCLC and can promote the occurrence of bone metastasis 50 . Through genome-wide differential expression gene screening, Wang et al observed that PNMA5 was significantly differentially expressed in glioblastoma cancer tissues, and it was closely related to the prognosis of glioblastoma 51 . The prognosis of glioblastoma patients with high expression of PNMA5 was poor 51 .…”

Section: Discussionmentioning

confidence: 99%

“…Through genome-wide differential expression gene screening, Wang et al observed that PNMA5 was significantly differentially expressed in glioblastoma cancer tissues, and it was closely related to the prognosis of glioblastoma 51 . The prognosis of glioblastoma patients with high expression of PNMA5 was poor 51 . Based on the TCGA colon cancer cohort, Zhou et al found that PNMA5 is significantly differentially expressed in colon cancer, and high expression of PNMA5 is associated with poor prognosis of colon cancer.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma

Liao¹,

Gu²,

Huang³

et al. 2023

J. Cancer

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Objective : Through data analysis, we observed that AC096751.1 is markedly imbalance between colon adenocarcinoma (COAD) cancer and paracancerous tissues. However, the prognostic value and potential molecular mechanism of AC096751.1 in COAD are still unclear. Methods: Whole genome RNA-sequencing datasets of The Cancer Genome Atlas (TCGA) COAD cohort were collected into current study, comprehensive survival analysis and bioinformatics function enrichment analysis approaches were apply to explore the clinical outcome and molecular mechanisms of AC096751.1 in COAD. Results: In current study, we found that AC096751.1 is markedly down-regulated in COAD cancer tissues (log2 fold change =2.303, P <0.0001, false discovery rate <0.0001), and can be serve as a biomarker to distinguish COAD cancer and paracancerous tissues [area under curve=0.9518, 95% confidence interval (CI)=0.9261-0.9776]. Survival analysis suggests that low expression of AC096751.1 is connected with poor clinical outcome of COAD, and can serve as a novel prognostic indicator (log-rank P=0.016, adjusted P=0.005, hazard ratio=0.548, 95% CI=0.360-0.836). Bioinformatics function enrichment analysis suggests that the molecular mechanism of AC096751.1 in COAD may include participation in cell adhesion, cell proliferation, mitogen-activated protein kinase kinase (MAPKK), MAPK, janus-activated kinase-singal transducers and activators of transcriprion cascade, Erk1 and Erk 2 cascade, and nuclear factor-kappa B pathway. Tumor microenvironment and immune infiltration analysis indicates that COAD patients with different AC096751.1 expression have significant variation in tumor immune background. Conclusion: The present study found that AC096751.1 is significantly differentially expressed in COAD and can be serve as a novel prognostic biomarker.

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“…Evidence has shown that p21-activated kinase (PAK7) regulates carcinogenesis in a variety of malignancies ( Gu et al, 2013 ; Han et al, 2014 ; Quan et al, 2020 ; Wang et al, 2020 ). Suppression of PAK7 increased radio-sensitivity in hepatocellular carcinoma (HCC) ( Gu and Kong, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Targeting immune cell types of tumor microenvironment to overcome resistance to PD-1/PD-L1 blockade in lung cancer

Wang

Zhu²,

Yang³

et al. 2023

Front. Pharmacol.

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Lung cancer is the common malignant tumor with the highest mortality rate. Lung cancer patients have achieved benefits from immunotherapy, including immune checkpoint inhibitors (ICIs) therapy. Unfortunately, cancer patients acquire adaptive immune resistance, leading to poor prognosis. Tumor microenvironment (TME) has been demonstrated to play a critical role in participating in acquired adaptive immune resistance. TME is associated with molecular heterogeneity of immunotherapy efficacy in lung cancer. In this article, we discuss how immune cell types of TME are correlated with immunotherapy in lung cancer. Moreover, we describe the efficacy of immunotherapy in driven gene mutations in lung cancer, including KRAS, TP53, EGFR, ALK, ROS1, KEAP1, ZFHX3, PTCH1, PAK7, UBE3A, TNF-α, NOTCH, LRP1B, FBXW7, and STK11. We also emphasize that modulation of immune cell types of TME could be a promising strategy for improving adaptive immune resistance in lung cancer.

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Bioinformatic analysis of the potential molecular mechanism of PAK7 expression in glioblastoma

Cited by 4 publications

References 54 publications

Antibody Profiling and In Silico Functional Analysis of Differentially Reactive Antibody Signatures of Glioblastomas and Meningiomas

Antibody Profiling and In Silico Functional Analysis of Differentially Reactive Antibody Signatures of Glioblastomas and Meningiomas

Genome-wide RNA-sequencing dataset reveals AC096751.1 sever as a novel prognostic long non-coding RNA and its potential molecular mechanisms in patients with colon adenocarcinoma

Targeting immune cell types of tumor microenvironment to overcome resistance to PD-1/PD-L1 blockade in lung cancer

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