Biological degradation of aflatoxin B1 by Rhodococcus erythropolis cultures

Alberts, J.F.; Engelbrecht, Y; Steyn, Pieter S.; Holzapfel, W.H.; Zyl, Willem H. van

doi:10.1016/j.ijfoodmicro.2006.01.019

Cited by 217 publications

(168 citation statements)

References 33 publications

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“…M. indica extract was found least effective among all the extracts used. Present results are in harmony with the fi ndings of Kumar et al (2005) and Alberts et al (2006). They reported that pre-and post-harvest bio-deterioration of crop seeds are mainly due to seed infestation by microorganisms and insects, causing up to 100% losses.…”

Section: Discussionsupporting

confidence: 89%

Chemical composition and antifungal potential of medicinal plants against seedborne mycoflora of eggplant (Solanum melongena L.)

Ashiq¹,

Chohan²,

Perveen³

et al. 2017

Acta Botanica Croatica

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-Antifungal activities of medicinal plants were observed against seedborne mycofl ora of eggplant (Solanum melongena). The effect of ethanolic leaf extracts of Mangifera indica, Mentha spicata, Citrus limon, Eucalyptus camaldulensis against four isolated fungal species including Fusarium oxysporum, Aspergillus fl avus, Rhizopus stolonifer and Penicillium digitatum was evaluated at various concentrations, by using the poisoned food technique. The impact of the extracts on seed germination and growth of eggplant was assessed by seed treatment and growth in a greenhouse experiment. Total fl avonoids of E. camaldulensis were analyzed through spectrophotometer, using quercetin as a standard. Physico-chemical parameters were also determined. Antifungal activity showed that maximum inhibition percentage of P. digitatum (67.78%) and F. oxysporum (64.44%) was observed at the highest concentration (80%) of C. limon and E. camaldulensis extracts, respectively, followed by M. spicata extract against A. fl avus (63.33%) and R. stolonifer (52.22%). Least inhibition percentage of F. oxysporum, P. digitatum, R. stolonifer and A. fl avus was 6.67, 7.78, 14.44 and 16.67%, respectively, at the lowest (20%) concentration of M. spicata. The greenhouse experiment showed variations in seedling germination and post-germination growth. E. camaldulensis extract showed an increase in percent germination (78.98%) over untreated control (62.83%), root and shoot length and fresh and dry weight of root and shoot with the consequent reduction in disease symptoms. Phytochemical analysis depicted the presence of alkaloids, fl avonoids, tannins, saponins in all extracts while steroids and glycosides were absent. A fair amount (10.38 mg QE g -1 DF ) of fl avonoid was present in leaf extract of E. camaldulensis. Physico-chemical analysis showed pH of 4.6, ash content of 0.41% and weight loss on drying of 8.14%.

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Section: Discussionsupporting

confidence: 89%

Chemical composition and antifungal potential of medicinal plants against seedborne mycoflora of eggplant (Solanum melongena L.)

Ashiq¹,

Chohan²,

Perveen³

et al. 2017

Acta Botanica Croatica

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“…Currently, R. erythropolis is mainly used to desulfurize diesel fuels (Zhang, 2007). Previous studies have shown that R. erythropolis effectively degraded aflatoxin B 1 (Alberts et al, 2006). In our laboratory, we found the more viable R. erythropolis in the culture, and the less aflatoxin B 1 remained.…”

Section: Introductionsupporting

confidence: 54%

Statistical experimental methods for optimizing the cultivating conditions for Rhodococcus erythropolis

Zhai¹,

Kong²,

Guan³

et al. 2011

Afr. J. Biotechnol.

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Rhodococcus erythropolis was found to effectively degrade aflatoxin B l produced by Aspergillus flavus and Aspergillus parasiticus. However, one problem of concern was the slow growth of this strain. In this study, Plackett-Burman design was used to select the most important variables, namely, temperature, pH, inoculum size, liquid volume, agitation speed and culture time that affected the growth of R. erythropolis. Central composite experimental design and response surface analysis were adopted to derive a statistical model for optimizing the culture conditions. From the obtained results, it can be concluded that the optimum parameters were: temperature, 15.3°C; pH, 5.56; inoculum size, 4%; liquid volume, 70 ml in 250 ml flask; agitation speed, 180 rpm; and culture time, 58.2 h. At this optimum point, the populations of the viable organisms could reach 10 8 colony forming units (CFU)/ml, which was 100 times higher than that incubated under the initial conditions. After 58.2 h incubation in this optimum cultivating conditions, 53.9 ± 2.1% of aflatoxin B 1 was degraded, while only 20.6±1.4% of aflatoxin B 1 was degraded in the initial conditions.

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“…Aflatoxins and fumonisins are the mycotoxins, in cereals, of major health and economic concern in the developing world [23,24,48,54,66,68,69]. Table 4 shows the deaths linked to mycotoxins in foods.…”

Section: Reduction Binding or Detoxification Of Mycotoxins In Fermenmentioning

confidence: 99%

Cereal-Based Functional Foods

Nyanzi¹,

Jooste²

2012

Probiotics

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Biological degradation of aflatoxin B1 by Rhodococcus erythropolis cultures

Cited by 217 publications

References 33 publications

Chemical composition and antifungal potential of medicinal plants against seedborne mycoflora of eggplant (Solanum melongena L.)

Chemical composition and antifungal potential of medicinal plants against seedborne mycoflora of eggplant (Solanum melongena L.)

Statistical experimental methods for optimizing the cultivating conditions for Rhodococcus erythropolis

Cereal-Based Functional Foods

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