Biophysical Effects of a Polymeric Biosurfactant in Candida krusei and Candida albicans Cells

Ferreira, Gabriella Freitas; Pinto, Bruna Lorrana dos Santos; Souza, Eliana Barbosa de; Viana, José Lima; Zagmignan, Adrielle; Santos, Julliana Ribeiro Alves; Santos, Áquila Rodrigues Costa; Tavares, Priscila Batista; Denadai, Ângelo Márcio Leite; Monteiro, Andrea de Souza

doi:10.1007/s11046-016-0054-z

Cited by 19 publications

(9 citation statements)

References 24 publications

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“…In this way, BS could induce changes in the cell surface characteristics and modify the interface contributing to the inhibition of the adherence of yeast to the epithelial cells. In addition, the cell surface differences in carbohydrate and protein concentrations between the Candida strains could contribute to the modulation of cell adhesion [57].…”

Section: Discussionmentioning

confidence: 99%

Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion

et al. 2020

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Background: Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to the prevention of urogenital and sexually transmitted infections. Their protective role in the vagina can be mediated by Lactobacillus cells themselves, metabolites or bacterial components, able to interfere with pathogen adhesion and infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused by the overgrowth of opportunistic Candida spp. including C. albicans, C. glabrata, C. krusei and C. tropicalis. Azole antifungal drugs are not always efficient in resolving VVC and preventing recurrent infections, thus alternative anti-Candida agents based on vaginal probiotics have gained more importance. The present work aims to chemically characterize the biosurfactant (BS) isolated from a vaginal Lactobacillus crispatus strain, L. crispatus BC1, and to investigate its safety and antiadhesive/antimicrobial activity against Candida spp., employing in vitro and in vivo assays. Results: BS isolated from vaginal L. crispatus BC1 was characterised as non-homogeneous lipopeptide molecules with a critical micellar concentration value of 2 mg/mL, and good emulsification and mucoadhesive properties. At 1.25 mg/mL, the BS was not cytotoxic and reduced Candida strains' ability to adhere to human cervical epithelial cells, mainly by exclusion mechanism. Moreover, intravaginal (i.va.) inoculation of BS in a murine experimental model was safe and did not perturb vaginal cytology, histology and cultivable vaginal microbiota. In the case of i.va. challenge of mice with C. albicans, BS was able to reduce leukocyte influx. Conclusions: These results indicate that BS from vaginal L. crispatus BC1 is able to interfere with Candida adhesion in vitro and in vivo, and suggest its potential as a preventive agent to reduce mucosal damage occasioned by Candida during VVC.

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Section: Discussionmentioning

confidence: 99%

Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion

et al. 2020

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“…Increases in the antimicrobial activity were associated with alkyl chain elongation [22]. The supposed interpretation of the SCGS antibacterial activity was attributed to an electrostatic interaction between the positive ammonium group, R 4 N + of the SCGS, and the negatively charged lipoprotein of the bacterial cell membrane, which leads to cell disruption [23]. In addition, the hydrophobic chain of the SCGS easily penetrated the microbial cell membrane, which led to damage of the cell's selective permeability and, consequently, the death of the cells [10].…”

Section: Staphylococcusmentioning

confidence: 99%

Multiple Applications of a Novel Cationic Gemini Surfactant: Anti-Microbial, Anti-Biofilm, Biocide, Salinity Corrosion Inhibitor, and Biofilm Dispersion (Part II)

et al. 2020

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The Egyptian petroleum industries are incurring severe problems with corrosion, particularly corrosion that is induced by sulfidogenic microbial activities in harsh salinity environments despite extensively using biocides and metal corrosion inhibitors. Therefore, in this study, a synthesized cationic gemini surfactant (SCGS) was tested as a broad-spectrum antimicrobial, anti-bacterial, anti-candida, anti-fungal, anti-biofilm (anti-adhesive), and bio-dispersion agent. The SCGS was evaluated as a biocide against environmental sulfidogenic-bacteria and as a corrosion inhibitor for a high salinity cultivated medium. The SCGS displayed wide spectrum antimicrobial activity with minimum bactericidal/fungicidal inhibitory concentrations. The SCGS demonstrated anti-bacterial, anti-biofilm, and bio-dispersion activity. The SCGS exhibited bactericidal activity against environmental sulfidogenic bacteria and the highest corrosion inhibition efficiency of 93.8% at 5 mM. Additionally, the SCGS demonstrated bio-dispersion activity against the environmental sulfidogenic bacteria at 5.49% salinity. In conclusion, this study provides a novel synthesized cationic surfactant with many applications in the oil and gas industry: as broad-spectrum antimicrobial and anti-biofilm agents, corrosion inhibition for high salinity, biocides for environmentally sulfidogenic bacteria, and as bio-dispersion agents.

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“…Much research has reported the antimicrobial activity of synthesized cationic surfactants that have 10 or 12 carbon atoms in their alkyl chain (Liu et al, 2015;Murguía et al, 2008;Obłąk et al, 2013). The supposed interpretation of the synthesized Gemini cationic surfactants' antibacterial susceptibility was attributed to an electrostatic interaction between the positive ammonium group, R 4 N + of the synthesized compounds, and the negatively charged lipoprotein of the bacterial cell membrane, which leads to cell disruption (Ferreira et al, 2016). In addition, the hydrophobic chain of the synthesized Gemini cationic surfactants can easily penetrate the bacterial cell membrane, leading to damage of its selective permeability and, consequently, death of the cells (Cornellas et al, 2011).…”

Section: Application Of the Synthesized Fhpao Fhpad And Fhpah Geminmentioning

confidence: 99%

Novel Gemini Cationic Surfactants: Thermodynamic, Antimicrobial Susceptibility, and Corrosion Inhibition Behavior against Acidithiobacillus ferrooxidans

Labena

Hamed

Ismael

et al. 2020

J Surfact & Detergents

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The Egyptian oil and gas industry is suffering from severe metal corrosion problems, particularly microbial‐induced corrosion. There is limited knowledge on the corrosion inhibition of carbon steels in the presence of an acidophilic, iron‐oxidizing bacterial species Acidithiobacillus ferrooxidans. Therefore, in this study, novel Gemini cationic surfactants, in three forms depending on variation in alkyl chains of 8, 12, and 16 carbon atoms named FHPAO, FHPAD, and FHPAH, respectively, were synthesized and characterized by Fourier transform infrared and nuclear magnetic resonance spectroscopy. The surface parameters and the thermodynamic of the synthesized surfactants were evaluated at three different temperatures, 20, 40, and 60 °C. The synthesized Gemini cationic surfactants were tested as broad‐spectrum antimicrobial, antibacterial and anticandida agents. They evaluated as biocides and corrosion inhibitors against Acidithiobacillus ferrooxidans. FHPAD showed higher adsorption ability at the solution interface and higher affinity to construct micelles than FHPAO and FHPAH. Both adsorption and micellization processes were hydrophobic and temperature dependent. FHPAO, FHPAD and FHPAH exhibited wide‐spectrum antimicrobial activities, and the highest activity and the lowest minimum bactericidal/fungicidal inhibitory concentrations were attributed to FHPAD. Furthermore, synthesized FHPAD demonstrated the highest metal corrosion inhibition efficiency of 95.5% at 5 mM in comparison to 87.5% and 81.7% for FHPAO and FHPAH, respectively. In conclusion, this study provides novel synthesized cationic surfactants with many applications in the oil and gas industry, such as broad‐spectrum antimicrobial, biocides, and corrosion inhibitors for acidophilic, iron‐oxidizing bacterial species Acidithiobacillus ferrooxidans.

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Biophysical Effects of a Polymeric Biosurfactant in Candida krusei and Candida albicans Cells

Cited by 19 publications

References 24 publications

Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion

Biosurfactant from vaginal Lactobacillus crispatus BC1 as a promising agent to interfere with Candida adhesion

Multiple Applications of a Novel Cationic Gemini Surfactant: Anti-Microbial, Anti-Biofilm, Biocide, Salinity Corrosion Inhibitor, and Biofilm Dispersion (Part II)

Novel Gemini Cationic Surfactants: Thermodynamic, Antimicrobial Susceptibility, and Corrosion Inhibition Behavior against Acidithiobacillus ferrooxidans

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