Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms

Sathya, S.; Parthasarathi, Shanmugam; Yun, Kyusik

doi:10.1016/j.colsurfb.2010.07.036

Cited by 229 publications

(114 citation statements)

References 36 publications

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“…The biosynthesized Ag NPs and ZnO NPs were freezedried and powdered in order to analyze XRD pattern (Sadhasivam et al 2010). The phase formation and purity of metallic nanoparticles were checked through XRD patterns which were recorded using powder X-ray diffractometer (Model-D8 Advance, made in BRUKER Germany).…”

Section: X-ray Diffraction Pattern Analysismentioning

confidence: 99%

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Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

2014

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The development of eco-friendly alternative to chemical synthesis of metal nanoparticles is of great challenge among researchers. The present study aimed to investigate the biological synthesis, characterization, antimicrobial study and synergistic effect of silver and zinc oxide nanoparticles against clinical pathogens using Pichia fermentans JA2. The extracellular biosynthesis of silver and zinc oxide nanoparticles was investigated using Pichia fermentans JA2 isolated from spoiled fruit pulp bought in Vellore local market. The crystalline and stable metallic nanoparticles were characterized evolving several analytical techniques including UV-visible spectrophotometer, X-ray diffraction pattern analysis and FE-scanning electron microscope with EDX-analysis. The biosynthesized metallic nanoparticles were tested for their antimicrobial property against medically important Gram positive, Gram negative and fungal pathogenic microorganisms. Furthermore, the biosynthesized nanoparticles were also evaluated for their increased antimicrobial activities with various commercially available antibiotics against clinical pathogens. The biosynthesized silver nanoparticles inhibited most of the Gram negative clinical pathogens, whereas zinc oxide nanoparticles were able to inhibit only Pseudomonas aeruginosa. The combined effect of standard antibiotic disc and biosynthesized metallic nanoparticles enhanced the inhibitory effect against clinical pathogens. The biological synthesis of silver and zinc oxide nanoparticles is a novel and cost-effective approach over harmful chemical synthesis techniques. The metallic nanoparticles synthesized using Pichia fermentans JA2 possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations.

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Section: X-ray Diffraction Pattern Analysismentioning

confidence: 99%

“…The mechanism of the biosynthesized nanoparticles involves the reduction of silver ions by the electron shuttle enzymatic metal reduction process (Sadhasivam et al 2010). The mechanism of the biosynthesized nanoparticles involves the reduction of silver ions by the electron shuttle enzymatic metal reduction process.…”

Section: Uv-visible Spectrophotometermentioning

confidence: 99%

Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

2014

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“…Strong antibacterial features of Ag are associated with the ability of this metal to interact with sulfhydryl groups of proteins as well as with DNA [30]. Ag nanoparticles have activity against Gram-positive and Gram-negative bacteria, fungi, and viruses [31,32].…”

Section: Discussionmentioning

confidence: 99%

In vitro biocompatibility of anodized titanium with deposited silver nanodendrites

et al. 2016

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Engineers searching new dental biomaterials try to modify the structure of the material in order to achieve the best performance as well as increased migration and proliferation of cells involved in the osseointegration of the implant. In this work we show in vitro test results of the Ti, which was anodically oxidized at high voltages with additionally deposited silver in the form of nanodendrites. The in vitro cytocompatibility of these materials was evaluated and compared with a conventional microcrystalline titanium. During the studies, established cell line of human gingival fibroblasts (HGF) and osteoblasts were cultured in the presence of tested materials, and its survival rate and proliferation activity were examined. Titanium samples modified with silver has a higher degree of biocompatibility in comparison with the unmodified reference material. Cells in contact with studied material showed a higher relative viability potential, stable level of proliferation activity, and lower rate of mortality. Biocompatibility tests carried out indicate that the anodically oxidized titanium at high voltages with additionally deposited nanosilver could be a possible candidate for dental implants and other medicinal applications.

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“…The zeta potential of our nanoparticle is -21.3mV, which shows nanoparticle are of highly stable, which are not having tendency to repel each other , it can assemble easily. [1], [3], [17]. The antibacterial activity of biosynthesized silver nanoparticle was investigated against various bacterial pathogens such as Klebsiellapneumoniae(MTCC 1687), Proteus vulgaris(MTCC 3160), Salmonella typhi(MTCC 3231), Shigella dysenteriae(MTCC 3642), Vibrio cholerae(MTCC 3906)using agar well diffusion method.…”

Section: IIImentioning

confidence: 99%

“…In which potential antimicrobial drugs are invaded in the mode of green chemistry approach [2]. It was reported in the earlier research, since from the ancient period the silver used as antimicrobial agent [3]. Whereas silver nanoparticles plays special role in antimicrobial activity with the special characteristics of small size and high surface area to volume ratio [4].…”

Section: Introductionmentioning

confidence: 99%

Extracellular Synthesis of Bactericidal Silver Nanoparticles Using Actinobacterium Dagang 5

Deepika¹

2017

IJRASET

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Abstract:Biologically synthesised silver nanoparticle using microbes are potential, cost effective and sustainable. Basically actinomycetes are popular for antibiotic and other therapeutic needs. In the present study the silver nanoparticle was synthesised using Actinobacteriumdagang 5 from marine sediments. The silver nitrate was treated with cell free supernatant of Actinobacteriumdagang 5 to synthesis the bactericidal silver nanoparticle and the initial characterisation was performed by observation of color change to intense brown color, UV-Vis spectrum of nanoparticle exhibited an absorption peak at 420nm that indicate surface Plasmon resonance of nanoparticles, X-Ray Diffraction (XRD) patterns showed the distinct peaks, and confirmsthat the nanoparticles are crystalline in nature at average size of 39.28nm. Fourier Transform Infrared Spectroscopy (FT-IR) analysis reveals the evidence, the protein acts as reducing and capping agents. Field Emission -Scanning Electron Microscope (FE-SEM) images showed the formation of nanoparticle. Energy Dispersive X-ray (EDAX) spectra register the presence of silver by showing as major signal. The stability of synthesised nanoparticle was analysed, the zeta potential of our nanoparticle is -21.3mV, which showed nanoparticle are highly stable. The biologically synthesised silver nanoparticle exemplified potential bactericidal efficacy towards the bacterial pathogens. From this study connote that Actinobacteriumdagang 5 is more desirable for the synthesis of Silver nanoparticle by extracellular method in the mode of efficient and eco friendly.

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Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms

Cited by 229 publications

References 36 publications

Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

In vitro biocompatibility of anodized titanium with deposited silver nanodendrites

Extracellular Synthesis of Bactericidal Silver Nanoparticles Using Actinobacterium Dagang 5

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