Biotechnological interventions for propagation, conservation and improvement of ‘Lantern Flowers’ (Ceropegia spp.)

Chavan, Jaykumar J.; Gaikwad, Nikhil B.; Dixit, G. B.; Yadav, Shrirang R.; Bapat, Vishwas A.

doi:10.1016/j.sajb.2017.10.021

Cited by 24 publications

(23 citation statements)

References 60 publications

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“…Rooting of the microshoots of most of the Ceropegia spp. was achieved through the incorporation of auxins especially IBA in the culture media [5]. Lower concentration of IAA (0.5 mg/l) also produced significant numbers of roots; however, the enhanced concentrations resulted in production of callus at the cut ends of microshoots.…”

Section: Resultsmentioning

confidence: 99%

“…The transplantation of in vitro raised plantlets into natural habitats is viable approach for plant conservation and restoration [5, 16]. In the current investigation, in vitro raised plantlets were kept in the glasshouse conditions for large-scale hardening (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The group is very interesting from the diversity, rarity, phytogeography, and pollination biology point of view [2]. Moreover, many species have nutritional, pharmacological, ornamental, and medicinal implications [4, 5].…”

Section: Introductionmentioning

confidence: 99%

“…So there is an urgent need to check the depletion of the natural population at the earliest by the implication of the latest available biotechnological tools. Plant tissue culture is one of the viable routes for large-scale propagation, conservation, and restoration of threatened plants [5, 7]. In the present study, in vitro regeneration system including shoot multiplication, rooting, and hardening has been developed for C. mohanramii .…”

Section: Introductionmentioning

confidence: 99%

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In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

Adsul

Chavan²,

Gaikwad

et al. 2019

Journal of Genetic Engineering and Biotechnology

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The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

Adsul

Chavan²,

Gaikwad

et al. 2019

Journal of Genetic Engineering and Biotechnology

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“…In this context, the conventional method of proliferation of tubers from roots is laborintensive and insufficient to meet the increasing population demand (Chavan et al, 2018). This study intended to develop an efficient protocol for in vitro conservation and regeneration of the germplasm of C. esculenta, as a contribution to implement strategies for the preservation of this valuable plant genetic resource.…”

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confidence: 99%

In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

Mancilla-Álvarez¹,

Ramírez-Mosqueda²,

Arano-Avalos³

et al. 2019

horts

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Malanga (Colocasia esculenta) is a plant genetic resource that requires biotechnological strategies for conservation and propagation. One time-, labor-, and space-saving option is in vitro conservation and regeneration. The objective of this study was to develop a protocol for in vitro regeneration and conservation of germplasm of C. esculenta var. criolla. For conservation through minimal growth, we assessed several concentrations of Murashige and Skoog (MS) medium (one-third, one-half, and three-quarter strength), the growth retardant ancymidol (0, 1, 2, and 3 mg·L−1), and the osmoregulator polyethylene glycol (PEG-8000 mw) at different concentrations (0, 10, 20, and 30 g·L−1). For in vitro conservation, the percent survival, shoot number and length, and number of leaves and roots per explant were evaluated after 24 weeks. For in vitro regeneration, different concentrations of thidiazuron (TDZ: 0, 0.5, 1, 1.5, and 2 mg·L−1) and 6-benzylaminopurine (BAP; 0, 1, 2, 3, and 4 mg·L−1) were evaluated. After 4 weeks of cultivation, the percent response, shoot number, and number of leaves per explant were recorded. During in vitro conservation, it was noted that the treatment including 2 mg·L−1 ancymidol resulted in a retarded development, without affecting the survival of the C. esculenta germplasm. With regard to shoot regeneration, 7.60 shoots per explant were obtained using 2 mg·L−1 TDZ. Finally, 98% survival was achieved during the acclimatization process. This study will contribute to the establishment of genetic improvement programs through in vitro conservation and propagation of this valuable plant genetic resource.

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First report of Trichoderma lixii causing leaf spot of Ceropegia vincaefolia in India

Bhujbal,

Patil,

Kadam

et al. 2024

New Disease Reports

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Lantern flower, Trichoderma Lantern flower (Ceropegia vincaefolia, Apocynaceae) is a perennial herbaceous climber which is endemic to the Western Ghats mountain range, especially the Rajasthan, Maharashtra and Kerala states of India. The tubers of C. vincaefolia have nutritional value due to their starch, gum, sugar, albuminoid, fat, crude fibre and mineral content, and the leaves are used as a leafy vegetable (Chavan et al., 2018). The flow-F I G U R E 1 Leaf spot caused by Trichoderma lixii on leaves of Ceropegia vincaefolia ers have horticultural value due to their unique phenotype and diverse colouring of the flowers (Chavan et al., 2018).

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Biotechnological interventions for propagation, conservation and improvement of ‘Lantern Flowers’ (Ceropegia spp.)

Cited by 24 publications

References 60 publications

In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

First report of Trichoderma lixii causing leaf spot of Ceropegia vincaefolia in India

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