Pestalotioid fungi, generally comprising Bartalinia, Monochaetia, Pestalotia, Pestalotiopsis, Sarcostroma, Seimatosporium, Truncatella, are coelomycetous genera with saprobic, endophytic or plant pathogenic life styles residing in the Amphisphaeriaceae (Xylariales). Little is known about the biodiversity of pestalotioid fungi in Iran. We provide a literature-based checklist for the pestalotioid fungi known to occur on different plant species in Iran. Two species, Bartalinia pondoensis and Pestalotiopsis neglecta are characterised based on morphological and molecular data from bamboo and rock samples, respectively. This is the first record of the genus Bartalinia from Iran and first report on the occurrence of B. pondoensis on bamboo and first report of P. neglecta on rock sample worldwide.